Cargando…

The subiculum is a patchwork of discrete subregions

In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of discrete subclasses. Here,...

Descripción completa

Detalles Bibliográficos
Autores principales: Cembrowski, Mark S, Wang, Lihua, Lemire, Andrew L, Copeland, Monique, DiLisio, Salvatore F, Clements, Jody, Spruston, Nelson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226292/
https://www.ncbi.nlm.nih.gov/pubmed/30375971
http://dx.doi.org/10.7554/eLife.37701
_version_ 1783369930857512960
author Cembrowski, Mark S
Wang, Lihua
Lemire, Andrew L
Copeland, Monique
DiLisio, Salvatore F
Clements, Jody
Spruston, Nelson
author_facet Cembrowski, Mark S
Wang, Lihua
Lemire, Andrew L
Copeland, Monique
DiLisio, Salvatore F
Clements, Jody
Spruston, Nelson
author_sort Cembrowski, Mark S
collection PubMed
description In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of discrete subclasses. Here, we investigated the extent and organizational principles governing pyramidal cell heterogeneity throughout the mouse subiculum. Using single-cell RNA-seq, we find that the subiculum pyramidal cell population can be deconstructed into eight separable subclasses. These subclasses were mapped onto abutting spatial domains, ultimately producing a complex laminar and columnar organization with heterogeneity across classical dorsal-ventral, proximal-distal, and superficial-deep axes. We further show that these transcriptomically defined subclasses correspond to differential protein products and can be associated with specific projection targets. This work deconstructs the complex landscape of subiculum pyramidal cells into spatially segregated subclasses that may be observed, controlled, and interpreted in future experiments.
format Online
Article
Text
id pubmed-6226292
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-62262922018-11-14 The subiculum is a patchwork of discrete subregions Cembrowski, Mark S Wang, Lihua Lemire, Andrew L Copeland, Monique DiLisio, Salvatore F Clements, Jody Spruston, Nelson eLife Neuroscience In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of discrete subclasses. Here, we investigated the extent and organizational principles governing pyramidal cell heterogeneity throughout the mouse subiculum. Using single-cell RNA-seq, we find that the subiculum pyramidal cell population can be deconstructed into eight separable subclasses. These subclasses were mapped onto abutting spatial domains, ultimately producing a complex laminar and columnar organization with heterogeneity across classical dorsal-ventral, proximal-distal, and superficial-deep axes. We further show that these transcriptomically defined subclasses correspond to differential protein products and can be associated with specific projection targets. This work deconstructs the complex landscape of subiculum pyramidal cells into spatially segregated subclasses that may be observed, controlled, and interpreted in future experiments. eLife Sciences Publications, Ltd 2018-10-30 /pmc/articles/PMC6226292/ /pubmed/30375971 http://dx.doi.org/10.7554/eLife.37701 Text en © 2018, Cembrowski et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Cembrowski, Mark S
Wang, Lihua
Lemire, Andrew L
Copeland, Monique
DiLisio, Salvatore F
Clements, Jody
Spruston, Nelson
The subiculum is a patchwork of discrete subregions
title The subiculum is a patchwork of discrete subregions
title_full The subiculum is a patchwork of discrete subregions
title_fullStr The subiculum is a patchwork of discrete subregions
title_full_unstemmed The subiculum is a patchwork of discrete subregions
title_short The subiculum is a patchwork of discrete subregions
title_sort subiculum is a patchwork of discrete subregions
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226292/
https://www.ncbi.nlm.nih.gov/pubmed/30375971
http://dx.doi.org/10.7554/eLife.37701
work_keys_str_mv AT cembrowskimarks thesubiculumisapatchworkofdiscretesubregions
AT wanglihua thesubiculumisapatchworkofdiscretesubregions
AT lemireandrewl thesubiculumisapatchworkofdiscretesubregions
AT copelandmonique thesubiculumisapatchworkofdiscretesubregions
AT dilisiosalvatoref thesubiculumisapatchworkofdiscretesubregions
AT clementsjody thesubiculumisapatchworkofdiscretesubregions
AT sprustonnelson thesubiculumisapatchworkofdiscretesubregions
AT cembrowskimarks subiculumisapatchworkofdiscretesubregions
AT wanglihua subiculumisapatchworkofdiscretesubregions
AT lemireandrewl subiculumisapatchworkofdiscretesubregions
AT copelandmonique subiculumisapatchworkofdiscretesubregions
AT dilisiosalvatoref subiculumisapatchworkofdiscretesubregions
AT clementsjody subiculumisapatchworkofdiscretesubregions
AT sprustonnelson subiculumisapatchworkofdiscretesubregions