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The subiculum is a patchwork of discrete subregions
In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of discrete subclasses. Here,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226292/ https://www.ncbi.nlm.nih.gov/pubmed/30375971 http://dx.doi.org/10.7554/eLife.37701 |
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author | Cembrowski, Mark S Wang, Lihua Lemire, Andrew L Copeland, Monique DiLisio, Salvatore F Clements, Jody Spruston, Nelson |
author_facet | Cembrowski, Mark S Wang, Lihua Lemire, Andrew L Copeland, Monique DiLisio, Salvatore F Clements, Jody Spruston, Nelson |
author_sort | Cembrowski, Mark S |
collection | PubMed |
description | In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of discrete subclasses. Here, we investigated the extent and organizational principles governing pyramidal cell heterogeneity throughout the mouse subiculum. Using single-cell RNA-seq, we find that the subiculum pyramidal cell population can be deconstructed into eight separable subclasses. These subclasses were mapped onto abutting spatial domains, ultimately producing a complex laminar and columnar organization with heterogeneity across classical dorsal-ventral, proximal-distal, and superficial-deep axes. We further show that these transcriptomically defined subclasses correspond to differential protein products and can be associated with specific projection targets. This work deconstructs the complex landscape of subiculum pyramidal cells into spatially segregated subclasses that may be observed, controlled, and interpreted in future experiments. |
format | Online Article Text |
id | pubmed-6226292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-62262922018-11-14 The subiculum is a patchwork of discrete subregions Cembrowski, Mark S Wang, Lihua Lemire, Andrew L Copeland, Monique DiLisio, Salvatore F Clements, Jody Spruston, Nelson eLife Neuroscience In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of discrete subclasses. Here, we investigated the extent and organizational principles governing pyramidal cell heterogeneity throughout the mouse subiculum. Using single-cell RNA-seq, we find that the subiculum pyramidal cell population can be deconstructed into eight separable subclasses. These subclasses were mapped onto abutting spatial domains, ultimately producing a complex laminar and columnar organization with heterogeneity across classical dorsal-ventral, proximal-distal, and superficial-deep axes. We further show that these transcriptomically defined subclasses correspond to differential protein products and can be associated with specific projection targets. This work deconstructs the complex landscape of subiculum pyramidal cells into spatially segregated subclasses that may be observed, controlled, and interpreted in future experiments. eLife Sciences Publications, Ltd 2018-10-30 /pmc/articles/PMC6226292/ /pubmed/30375971 http://dx.doi.org/10.7554/eLife.37701 Text en © 2018, Cembrowski et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Cembrowski, Mark S Wang, Lihua Lemire, Andrew L Copeland, Monique DiLisio, Salvatore F Clements, Jody Spruston, Nelson The subiculum is a patchwork of discrete subregions |
title | The subiculum is a patchwork of discrete subregions |
title_full | The subiculum is a patchwork of discrete subregions |
title_fullStr | The subiculum is a patchwork of discrete subregions |
title_full_unstemmed | The subiculum is a patchwork of discrete subregions |
title_short | The subiculum is a patchwork of discrete subregions |
title_sort | subiculum is a patchwork of discrete subregions |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226292/ https://www.ncbi.nlm.nih.gov/pubmed/30375971 http://dx.doi.org/10.7554/eLife.37701 |
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