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Active intermixing of indirect and direct neurons builds the striatal mosaic
The striatum controls behaviors via the activity of direct and indirect pathway projection neurons (dSPN and iSPN) that are intermingled in all compartments. While such cellular mosaic ensures the balanced activity of the two pathways, its developmental origin and pattern remains largely unknown. He...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226429/ https://www.ncbi.nlm.nih.gov/pubmed/30413696 http://dx.doi.org/10.1038/s41467-018-07171-4 |
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author | Tinterri, Andrea Menardy, Fabien Diana, Marco A. Lokmane, Ludmilla Keita, Maryama Coulpier, Fanny Lemoine, Sophie Mailhes, Caroline Mathieu, Benjamin Merchan-Sala, Paloma Campbell, Kenneth Gyory, Ildiko Grosschedl, Rudolf Popa, Daniela Garel, Sonia |
author_facet | Tinterri, Andrea Menardy, Fabien Diana, Marco A. Lokmane, Ludmilla Keita, Maryama Coulpier, Fanny Lemoine, Sophie Mailhes, Caroline Mathieu, Benjamin Merchan-Sala, Paloma Campbell, Kenneth Gyory, Ildiko Grosschedl, Rudolf Popa, Daniela Garel, Sonia |
author_sort | Tinterri, Andrea |
collection | PubMed |
description | The striatum controls behaviors via the activity of direct and indirect pathway projection neurons (dSPN and iSPN) that are intermingled in all compartments. While such cellular mosaic ensures the balanced activity of the two pathways, its developmental origin and pattern remains largely unknown. Here, we show that both SPN populations are specified embryonically and intermix progressively through multidirectional iSPN migration. Using conditional mutant mice, we found that inactivation of the dSPN-specific transcription factor Ebf1 impairs selective dSPN properties, including axon pathfinding, while molecular and functional features of iSPN were preserved. Ebf1 mutation disrupted iSPN/dSPN intermixing, resulting in an uneven distribution. Such architectural defect was selective of the matrix compartment, highlighting that intermixing is a parallel process to compartment formation. Our study reveals while iSPN/dSPN specification is largely independent, their intermingling emerges from an active migration of iSPN, thereby providing a novel framework for the building of striatal architecture. |
format | Online Article Text |
id | pubmed-6226429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62264292018-11-13 Active intermixing of indirect and direct neurons builds the striatal mosaic Tinterri, Andrea Menardy, Fabien Diana, Marco A. Lokmane, Ludmilla Keita, Maryama Coulpier, Fanny Lemoine, Sophie Mailhes, Caroline Mathieu, Benjamin Merchan-Sala, Paloma Campbell, Kenneth Gyory, Ildiko Grosschedl, Rudolf Popa, Daniela Garel, Sonia Nat Commun Article The striatum controls behaviors via the activity of direct and indirect pathway projection neurons (dSPN and iSPN) that are intermingled in all compartments. While such cellular mosaic ensures the balanced activity of the two pathways, its developmental origin and pattern remains largely unknown. Here, we show that both SPN populations are specified embryonically and intermix progressively through multidirectional iSPN migration. Using conditional mutant mice, we found that inactivation of the dSPN-specific transcription factor Ebf1 impairs selective dSPN properties, including axon pathfinding, while molecular and functional features of iSPN were preserved. Ebf1 mutation disrupted iSPN/dSPN intermixing, resulting in an uneven distribution. Such architectural defect was selective of the matrix compartment, highlighting that intermixing is a parallel process to compartment formation. Our study reveals while iSPN/dSPN specification is largely independent, their intermingling emerges from an active migration of iSPN, thereby providing a novel framework for the building of striatal architecture. Nature Publishing Group UK 2018-11-09 /pmc/articles/PMC6226429/ /pubmed/30413696 http://dx.doi.org/10.1038/s41467-018-07171-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tinterri, Andrea Menardy, Fabien Diana, Marco A. Lokmane, Ludmilla Keita, Maryama Coulpier, Fanny Lemoine, Sophie Mailhes, Caroline Mathieu, Benjamin Merchan-Sala, Paloma Campbell, Kenneth Gyory, Ildiko Grosschedl, Rudolf Popa, Daniela Garel, Sonia Active intermixing of indirect and direct neurons builds the striatal mosaic |
title | Active intermixing of indirect and direct neurons builds the striatal mosaic |
title_full | Active intermixing of indirect and direct neurons builds the striatal mosaic |
title_fullStr | Active intermixing of indirect and direct neurons builds the striatal mosaic |
title_full_unstemmed | Active intermixing of indirect and direct neurons builds the striatal mosaic |
title_short | Active intermixing of indirect and direct neurons builds the striatal mosaic |
title_sort | active intermixing of indirect and direct neurons builds the striatal mosaic |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226429/ https://www.ncbi.nlm.nih.gov/pubmed/30413696 http://dx.doi.org/10.1038/s41467-018-07171-4 |
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