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Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats
BACKGROUND: Neurological disorders suggest that the excitotoxicity involves a drastic increase in intracellular Ca(2+) concentrations and the formation of reactive oxygen species. The presence of these free radicals may also affect the dopaminergic system. The aim of this work was to determine if ri...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230234/ https://www.ncbi.nlm.nih.gov/pubmed/30413185 http://dx.doi.org/10.1186/s12868-018-0474-4 |
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author | Peraza, Armando Valenzuela Guzmán, David Calderón Brizuela, Norma Osnaya Herrera, Maribel Ortiz Olguín, Hugo Juárez Silva, Miroslava Lindoro Tapia, Belén Juárez Mejía, Gerardo Barragán |
author_facet | Peraza, Armando Valenzuela Guzmán, David Calderón Brizuela, Norma Osnaya Herrera, Maribel Ortiz Olguín, Hugo Juárez Silva, Miroslava Lindoro Tapia, Belén Juárez Mejía, Gerardo Barragán |
author_sort | Peraza, Armando Valenzuela |
collection | PubMed |
description | BACKGROUND: Neurological disorders suggest that the excitotoxicity involves a drastic increase in intracellular Ca(2+) concentrations and the formation of reactive oxygen species. The presence of these free radicals may also affect the dopaminergic system. The aim of this work was to determine if riboflavin (B(2)) and pyridoxine (B(6)) provide protection to the brain against free radicals generated by 3-nitropropionic acid (3-NPA) by measuring the levels of dopamine (DA) and selected oxidative stress markers. METHODS: Male Fisher rats were grouped (n = 6) and treated as follows: group 1, control (NaCl 0.9%); group 2, 3-NPA (20 mg/kg); group 3, B(2) (10 mg/kg); group 4, B(2) (10 mg/kg) + 3-NPA (20 mg/kg); group 5, B(6) (10 mg/kg) and group 6, B(6) + 3-NPA. All treatments were administered every 24 h for 5 days by intraperitoneal route. After sacrifice, the brain was obtained to measure DA, GSH, and lipid peroxidation, Ca(2+), Mg(2+), ATPase and H(2)O(2). MAIN FINDINGS: Levels of dopamine increased in cortex, striatum and cerebellum/medulla oblongata of animals that received 3-NPA alone. The lipid peroxidation increased in cortex, striatum, and cerebellum/medulla oblongata, of animals treated with B(2) vitamin alone. ATPase dependent on Ca(+2), Mg(+2) and H(2)O(2) increased in all regions of animals that received 3-NPA alone. CONCLUSION: The results confirm the capacity of 3-NPA to generate oxidative stress. Besides, the study suggests that B(2) or B(6) vitamins restored the levels of DA and reduced oxidative stress in brain of rats. We believe that these results would help in the study of neurodegenerative diseases. |
format | Online Article Text |
id | pubmed-6230234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62302342018-11-19 Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats Peraza, Armando Valenzuela Guzmán, David Calderón Brizuela, Norma Osnaya Herrera, Maribel Ortiz Olguín, Hugo Juárez Silva, Miroslava Lindoro Tapia, Belén Juárez Mejía, Gerardo Barragán BMC Neurosci Research Article BACKGROUND: Neurological disorders suggest that the excitotoxicity involves a drastic increase in intracellular Ca(2+) concentrations and the formation of reactive oxygen species. The presence of these free radicals may also affect the dopaminergic system. The aim of this work was to determine if riboflavin (B(2)) and pyridoxine (B(6)) provide protection to the brain against free radicals generated by 3-nitropropionic acid (3-NPA) by measuring the levels of dopamine (DA) and selected oxidative stress markers. METHODS: Male Fisher rats were grouped (n = 6) and treated as follows: group 1, control (NaCl 0.9%); group 2, 3-NPA (20 mg/kg); group 3, B(2) (10 mg/kg); group 4, B(2) (10 mg/kg) + 3-NPA (20 mg/kg); group 5, B(6) (10 mg/kg) and group 6, B(6) + 3-NPA. All treatments were administered every 24 h for 5 days by intraperitoneal route. After sacrifice, the brain was obtained to measure DA, GSH, and lipid peroxidation, Ca(2+), Mg(2+), ATPase and H(2)O(2). MAIN FINDINGS: Levels of dopamine increased in cortex, striatum and cerebellum/medulla oblongata of animals that received 3-NPA alone. The lipid peroxidation increased in cortex, striatum, and cerebellum/medulla oblongata, of animals treated with B(2) vitamin alone. ATPase dependent on Ca(+2), Mg(+2) and H(2)O(2) increased in all regions of animals that received 3-NPA alone. CONCLUSION: The results confirm the capacity of 3-NPA to generate oxidative stress. Besides, the study suggests that B(2) or B(6) vitamins restored the levels of DA and reduced oxidative stress in brain of rats. We believe that these results would help in the study of neurodegenerative diseases. BioMed Central 2018-11-09 /pmc/articles/PMC6230234/ /pubmed/30413185 http://dx.doi.org/10.1186/s12868-018-0474-4 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Peraza, Armando Valenzuela Guzmán, David Calderón Brizuela, Norma Osnaya Herrera, Maribel Ortiz Olguín, Hugo Juárez Silva, Miroslava Lindoro Tapia, Belén Juárez Mejía, Gerardo Barragán Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
title | Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
title_full | Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
title_fullStr | Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
title_full_unstemmed | Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
title_short | Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
title_sort | riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230234/ https://www.ncbi.nlm.nih.gov/pubmed/30413185 http://dx.doi.org/10.1186/s12868-018-0474-4 |
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