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Short and narrow flag leaf1, a GATA zinc finger domain-containing protein, regulates flag leaf size in rice (Oryza sativa)
BACKGROUND: The flag leaf of rice (Oryza sativa L.) is an important determinant of plant type characteristics and grain yield. Identification of flag leaf mutants of rice is crucial to elucidate the molecular mechanism of flag-leaf development, and for exploitation of rice germplasm resources. RESUL...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230254/ https://www.ncbi.nlm.nih.gov/pubmed/30413183 http://dx.doi.org/10.1186/s12870-018-1452-9 |
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author | He, Peilong Wang, Xiaowen Zhang, Xiaobo Jiang, Yudong Tian, Weijiang Zhang, Xiaoqiong Li, Yangyang Sun, Ying Xie, Jia Ni, Jile He, Guanghua Sang, Xianchun |
author_facet | He, Peilong Wang, Xiaowen Zhang, Xiaobo Jiang, Yudong Tian, Weijiang Zhang, Xiaoqiong Li, Yangyang Sun, Ying Xie, Jia Ni, Jile He, Guanghua Sang, Xianchun |
author_sort | He, Peilong |
collection | PubMed |
description | BACKGROUND: The flag leaf of rice (Oryza sativa L.) is an important determinant of plant type characteristics and grain yield. Identification of flag leaf mutants of rice is crucial to elucidate the molecular mechanism of flag-leaf development, and for exploitation of rice germplasm resources. RESULTS: In this study, we describe a mutant designated short and narrow flag leaf 1 (snfl1). Histological analysis showed that the length of epidermal cells and number of longitudinal veins were decreased in the flag leaf of the snfl1 mutant. Map-based cloning indicated that a member of the GATA family of transcription factors is a candidate gene for SNFL1. A single-nucleotide transition at the last base in the single intron of snfl1 led to variation in alternative splicing and early termination of translation. Complemented transgenic plants harbouring the candidate SNFL1 gene rescued the snfl1 mutant. Analysis of RT-PCR and the SNFL1 promoter by means of a GUS fusion expression assay showed that abundance of SNFL1 transcripts was higher in the culm, leaf sheath, and root. Expression of the SNFL1-GFP fusion protein in rice protoplasts showed that SNFL1 was localized in nucleus. CONCLUSIONS: We conclude that SNFL1 is an important regulator of leaf development, the identification of which might have important implications for future research on GATA transcription factors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1452-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6230254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62302542018-11-19 Short and narrow flag leaf1, a GATA zinc finger domain-containing protein, regulates flag leaf size in rice (Oryza sativa) He, Peilong Wang, Xiaowen Zhang, Xiaobo Jiang, Yudong Tian, Weijiang Zhang, Xiaoqiong Li, Yangyang Sun, Ying Xie, Jia Ni, Jile He, Guanghua Sang, Xianchun BMC Plant Biol Research Article BACKGROUND: The flag leaf of rice (Oryza sativa L.) is an important determinant of plant type characteristics and grain yield. Identification of flag leaf mutants of rice is crucial to elucidate the molecular mechanism of flag-leaf development, and for exploitation of rice germplasm resources. RESULTS: In this study, we describe a mutant designated short and narrow flag leaf 1 (snfl1). Histological analysis showed that the length of epidermal cells and number of longitudinal veins were decreased in the flag leaf of the snfl1 mutant. Map-based cloning indicated that a member of the GATA family of transcription factors is a candidate gene for SNFL1. A single-nucleotide transition at the last base in the single intron of snfl1 led to variation in alternative splicing and early termination of translation. Complemented transgenic plants harbouring the candidate SNFL1 gene rescued the snfl1 mutant. Analysis of RT-PCR and the SNFL1 promoter by means of a GUS fusion expression assay showed that abundance of SNFL1 transcripts was higher in the culm, leaf sheath, and root. Expression of the SNFL1-GFP fusion protein in rice protoplasts showed that SNFL1 was localized in nucleus. CONCLUSIONS: We conclude that SNFL1 is an important regulator of leaf development, the identification of which might have important implications for future research on GATA transcription factors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1452-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-09 /pmc/articles/PMC6230254/ /pubmed/30413183 http://dx.doi.org/10.1186/s12870-018-1452-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article He, Peilong Wang, Xiaowen Zhang, Xiaobo Jiang, Yudong Tian, Weijiang Zhang, Xiaoqiong Li, Yangyang Sun, Ying Xie, Jia Ni, Jile He, Guanghua Sang, Xianchun Short and narrow flag leaf1, a GATA zinc finger domain-containing protein, regulates flag leaf size in rice (Oryza sativa) |
title | Short and narrow flag leaf1, a GATA zinc finger domain-containing protein, regulates flag leaf size in rice (Oryza sativa) |
title_full | Short and narrow flag leaf1, a GATA zinc finger domain-containing protein, regulates flag leaf size in rice (Oryza sativa) |
title_fullStr | Short and narrow flag leaf1, a GATA zinc finger domain-containing protein, regulates flag leaf size in rice (Oryza sativa) |
title_full_unstemmed | Short and narrow flag leaf1, a GATA zinc finger domain-containing protein, regulates flag leaf size in rice (Oryza sativa) |
title_short | Short and narrow flag leaf1, a GATA zinc finger domain-containing protein, regulates flag leaf size in rice (Oryza sativa) |
title_sort | short and narrow flag leaf1, a gata zinc finger domain-containing protein, regulates flag leaf size in rice (oryza sativa) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230254/ https://www.ncbi.nlm.nih.gov/pubmed/30413183 http://dx.doi.org/10.1186/s12870-018-1452-9 |
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