Cargando…

Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus

The suprachiasmatic nucleus (SCN) is the principal pacemaker driving the circadian rhythms of physiological behaviors. The SCN consists of distinct neurons expressing neuropeptides, including arginine vasopressin (AVP), vasoactive intestinal polypeptide (VIP), gastrin-releasing peptide (GRP), cholec...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuan, Xiang-Shan, Wei, Hao-Hua, Xu, Wei, Wang, Lu, Qu, Wei-Min, Li, Rui-Xi, Huang, Zhi-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230653/
https://www.ncbi.nlm.nih.gov/pubmed/30455627
http://dx.doi.org/10.3389/fnins.2018.00807
_version_ 1783370120907718656
author Yuan, Xiang-Shan
Wei, Hao-Hua
Xu, Wei
Wang, Lu
Qu, Wei-Min
Li, Rui-Xi
Huang, Zhi-Li
author_facet Yuan, Xiang-Shan
Wei, Hao-Hua
Xu, Wei
Wang, Lu
Qu, Wei-Min
Li, Rui-Xi
Huang, Zhi-Li
author_sort Yuan, Xiang-Shan
collection PubMed
description The suprachiasmatic nucleus (SCN) is the principal pacemaker driving the circadian rhythms of physiological behaviors. The SCN consists of distinct neurons expressing neuropeptides, including arginine vasopressin (AVP), vasoactive intestinal polypeptide (VIP), gastrin-releasing peptide (GRP), cholecystokinin (CCK), and so on. AVP, VIP, and GRP neurons receive light stimulation from the retina to synchronize endogenous circadian clocks with the solar day, whereas CCK neurons are not directly innervated by retinal ganglion cells and may be involved in the non-photic regulation of the circadian clock. To better understand the function of CCK neurons in non-photic circadian rhythm, it is vital to clarify the direct afferent inputs to CCK neurons in the SCN. Here, we utilized a recently developed rabies virus- and Cre/loxP-based, cell type-specific, retrograde tracing system to map and quantitatively analyze the whole-brain monosynaptic inputs to SCN CCK neurons. We found that SCN CCK neurons received direct inputs from 29 brain nuclei. Among these nuclei, paraventricular nucleus of the hypothalamus (PVH), paraventricular nucleus of the thalamus (PVT), supraoptic nucleus (SON), ventromedial nucleus of the hypothalamus, and seven other nuclei sent numerous inputs to CCK neurons. Moderate inputs originated from the zona incerta, periventricular hypothalamic nucleus, and five other nuclei. A few inputs to CCK neurons originated from the orbital frontal cortex, prelimbic cortex, cingulate cortex, claustrum, and seven other nuclei. In addition, SCN CCK neurons were preferentially innervated by AVP neurons of the ipsilateral PVH and SON rather than their contralateral counterpart, whereas the contralateral PVT sent more projections to CCK neurons than to its ipsilateral counterpart. Taken together, these results expand our knowledge of the specific innervation to mouse SCN CCK neurons and provide an important indication for further investigations on the function of CCK neurons.
format Online
Article
Text
id pubmed-6230653
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-62306532018-11-19 Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus Yuan, Xiang-Shan Wei, Hao-Hua Xu, Wei Wang, Lu Qu, Wei-Min Li, Rui-Xi Huang, Zhi-Li Front Neurosci Neuroscience The suprachiasmatic nucleus (SCN) is the principal pacemaker driving the circadian rhythms of physiological behaviors. The SCN consists of distinct neurons expressing neuropeptides, including arginine vasopressin (AVP), vasoactive intestinal polypeptide (VIP), gastrin-releasing peptide (GRP), cholecystokinin (CCK), and so on. AVP, VIP, and GRP neurons receive light stimulation from the retina to synchronize endogenous circadian clocks with the solar day, whereas CCK neurons are not directly innervated by retinal ganglion cells and may be involved in the non-photic regulation of the circadian clock. To better understand the function of CCK neurons in non-photic circadian rhythm, it is vital to clarify the direct afferent inputs to CCK neurons in the SCN. Here, we utilized a recently developed rabies virus- and Cre/loxP-based, cell type-specific, retrograde tracing system to map and quantitatively analyze the whole-brain monosynaptic inputs to SCN CCK neurons. We found that SCN CCK neurons received direct inputs from 29 brain nuclei. Among these nuclei, paraventricular nucleus of the hypothalamus (PVH), paraventricular nucleus of the thalamus (PVT), supraoptic nucleus (SON), ventromedial nucleus of the hypothalamus, and seven other nuclei sent numerous inputs to CCK neurons. Moderate inputs originated from the zona incerta, periventricular hypothalamic nucleus, and five other nuclei. A few inputs to CCK neurons originated from the orbital frontal cortex, prelimbic cortex, cingulate cortex, claustrum, and seven other nuclei. In addition, SCN CCK neurons were preferentially innervated by AVP neurons of the ipsilateral PVH and SON rather than their contralateral counterpart, whereas the contralateral PVT sent more projections to CCK neurons than to its ipsilateral counterpart. Taken together, these results expand our knowledge of the specific innervation to mouse SCN CCK neurons and provide an important indication for further investigations on the function of CCK neurons. Frontiers Media S.A. 2018-11-05 /pmc/articles/PMC6230653/ /pubmed/30455627 http://dx.doi.org/10.3389/fnins.2018.00807 Text en Copyright © 2018 Yuan, Wei, Xu, Wang, Qu, Li and Huang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Yuan, Xiang-Shan
Wei, Hao-Hua
Xu, Wei
Wang, Lu
Qu, Wei-Min
Li, Rui-Xi
Huang, Zhi-Li
Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus
title Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus
title_full Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus
title_fullStr Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus
title_full_unstemmed Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus
title_short Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus
title_sort whole-brain monosynaptic afferent projections to the cholecystokinin neurons of the suprachiasmatic nucleus
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230653/
https://www.ncbi.nlm.nih.gov/pubmed/30455627
http://dx.doi.org/10.3389/fnins.2018.00807
work_keys_str_mv AT yuanxiangshan wholebrainmonosynapticafferentprojectionstothecholecystokininneuronsofthesuprachiasmaticnucleus
AT weihaohua wholebrainmonosynapticafferentprojectionstothecholecystokininneuronsofthesuprachiasmaticnucleus
AT xuwei wholebrainmonosynapticafferentprojectionstothecholecystokininneuronsofthesuprachiasmaticnucleus
AT wanglu wholebrainmonosynapticafferentprojectionstothecholecystokininneuronsofthesuprachiasmaticnucleus
AT quweimin wholebrainmonosynapticafferentprojectionstothecholecystokininneuronsofthesuprachiasmaticnucleus
AT liruixi wholebrainmonosynapticafferentprojectionstothecholecystokininneuronsofthesuprachiasmaticnucleus
AT huangzhili wholebrainmonosynapticafferentprojectionstothecholecystokininneuronsofthesuprachiasmaticnucleus