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IL-1R and Inflammasomes Mediate Early Pulmonary Protective Mechanisms in Respiratory Brucella Abortus Infection

Brucella spp. infection is frequently acquired through contaminated aerosols. The role of interleukin-1 beta (IL-1β) in the early pulmonary response to respiratory Brucella infection is unknown. As shown here, IL-1β levels in lung homogenates and bronchoalveolar lavage fluid (BALF) of mice intratrac...

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Autores principales: Hielpos, M. Soledad, Fernández, Andrea G., Falivene, Juliana, Alonso Paiva, Iván M., Muñoz González, Florencia, Ferrero, Mariana C., Campos, Priscila C., Vieira, Angelica T., Oliveira, Sergio Costa, Baldi, Pablo C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231193/
https://www.ncbi.nlm.nih.gov/pubmed/30456207
http://dx.doi.org/10.3389/fcimb.2018.00391
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author Hielpos, M. Soledad
Fernández, Andrea G.
Falivene, Juliana
Alonso Paiva, Iván M.
Muñoz González, Florencia
Ferrero, Mariana C.
Campos, Priscila C.
Vieira, Angelica T.
Oliveira, Sergio Costa
Baldi, Pablo C.
author_facet Hielpos, M. Soledad
Fernández, Andrea G.
Falivene, Juliana
Alonso Paiva, Iván M.
Muñoz González, Florencia
Ferrero, Mariana C.
Campos, Priscila C.
Vieira, Angelica T.
Oliveira, Sergio Costa
Baldi, Pablo C.
author_sort Hielpos, M. Soledad
collection PubMed
description Brucella spp. infection is frequently acquired through contaminated aerosols. The role of interleukin-1 beta (IL-1β) in the early pulmonary response to respiratory Brucella infection is unknown. As shown here, IL-1β levels in lung homogenates and bronchoalveolar lavage fluid (BALF) of mice intratracheally inoculated with B. abortus were increased at 3 and 7 days p.i. At 7 days p.i., pulmonary CFU numbers were higher in IL-1 receptor (IL-1R) knockout (KO) mice than in wild type (WT) mice. At different times p.i. CFU in lungs and BALF were higher in mice lacking some inflammasome components (caspase-1, AIM2, NLRP3) than in WT mice. At 2 days p.i. pulmonary levels of IL-1β and CXCL1 (neutrophils chemoattractant) were lower in caspase-1/11 KO mice. At day 3 p.i., neutrophils counts in BALF were lower in caspase-1/11 KO mice than in WT mice. During in vitro infections, IL-1β secretion was lower in alveolar macrophages from caspase-1/11, NLRP3 or AIM2 KO mice than in WT controls. Similarly, IL-1β production by B. abortus-infected alveolar epithelial cells was reduced by pretreatment with a specific caspase-1 inhibitor. This study shows that IL-1R, probably through IL-1β action, and the NLRP3 and AIM2 inflammasomes are involved in pulmonary innate immune protective mechanisms against respiratory B. abortus infection.
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spelling pubmed-62311932018-11-19 IL-1R and Inflammasomes Mediate Early Pulmonary Protective Mechanisms in Respiratory Brucella Abortus Infection Hielpos, M. Soledad Fernández, Andrea G. Falivene, Juliana Alonso Paiva, Iván M. Muñoz González, Florencia Ferrero, Mariana C. Campos, Priscila C. Vieira, Angelica T. Oliveira, Sergio Costa Baldi, Pablo C. Front Cell Infect Microbiol Cellular and Infection Microbiology Brucella spp. infection is frequently acquired through contaminated aerosols. The role of interleukin-1 beta (IL-1β) in the early pulmonary response to respiratory Brucella infection is unknown. As shown here, IL-1β levels in lung homogenates and bronchoalveolar lavage fluid (BALF) of mice intratracheally inoculated with B. abortus were increased at 3 and 7 days p.i. At 7 days p.i., pulmonary CFU numbers were higher in IL-1 receptor (IL-1R) knockout (KO) mice than in wild type (WT) mice. At different times p.i. CFU in lungs and BALF were higher in mice lacking some inflammasome components (caspase-1, AIM2, NLRP3) than in WT mice. At 2 days p.i. pulmonary levels of IL-1β and CXCL1 (neutrophils chemoattractant) were lower in caspase-1/11 KO mice. At day 3 p.i., neutrophils counts in BALF were lower in caspase-1/11 KO mice than in WT mice. During in vitro infections, IL-1β secretion was lower in alveolar macrophages from caspase-1/11, NLRP3 or AIM2 KO mice than in WT controls. Similarly, IL-1β production by B. abortus-infected alveolar epithelial cells was reduced by pretreatment with a specific caspase-1 inhibitor. This study shows that IL-1R, probably through IL-1β action, and the NLRP3 and AIM2 inflammasomes are involved in pulmonary innate immune protective mechanisms against respiratory B. abortus infection. Frontiers Media S.A. 2018-11-05 /pmc/articles/PMC6231193/ /pubmed/30456207 http://dx.doi.org/10.3389/fcimb.2018.00391 Text en Copyright © 2018 Hielpos, Fernández, Falivene, Alonso Paiva, Muñoz González, Ferrero, Campos, Vieira, Oliveira and Baldi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Hielpos, M. Soledad
Fernández, Andrea G.
Falivene, Juliana
Alonso Paiva, Iván M.
Muñoz González, Florencia
Ferrero, Mariana C.
Campos, Priscila C.
Vieira, Angelica T.
Oliveira, Sergio Costa
Baldi, Pablo C.
IL-1R and Inflammasomes Mediate Early Pulmonary Protective Mechanisms in Respiratory Brucella Abortus Infection
title IL-1R and Inflammasomes Mediate Early Pulmonary Protective Mechanisms in Respiratory Brucella Abortus Infection
title_full IL-1R and Inflammasomes Mediate Early Pulmonary Protective Mechanisms in Respiratory Brucella Abortus Infection
title_fullStr IL-1R and Inflammasomes Mediate Early Pulmonary Protective Mechanisms in Respiratory Brucella Abortus Infection
title_full_unstemmed IL-1R and Inflammasomes Mediate Early Pulmonary Protective Mechanisms in Respiratory Brucella Abortus Infection
title_short IL-1R and Inflammasomes Mediate Early Pulmonary Protective Mechanisms in Respiratory Brucella Abortus Infection
title_sort il-1r and inflammasomes mediate early pulmonary protective mechanisms in respiratory brucella abortus infection
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231193/
https://www.ncbi.nlm.nih.gov/pubmed/30456207
http://dx.doi.org/10.3389/fcimb.2018.00391
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