Cargando…
Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity
Cardiac development is a dynamic process and sensitive to environmental chemicals. Triclosan is widely used as an antibacterial agent and reported to transport across the placenta and affect embryonic development. Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to deter...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231387/ https://www.ncbi.nlm.nih.gov/pubmed/30510588 http://dx.doi.org/10.1155/2018/8608327 |
_version_ | 1783370214057967616 |
---|---|
author | Du, Guizhen Yu, Mingming Wang, Lingling Hu, Weiyue Song, Ling Lu, Chuncheng Wang, Xinru |
author_facet | Du, Guizhen Yu, Mingming Wang, Lingling Hu, Weiyue Song, Ling Lu, Chuncheng Wang, Xinru |
author_sort | Du, Guizhen |
collection | PubMed |
description | Cardiac development is a dynamic process and sensitive to environmental chemicals. Triclosan is widely used as an antibacterial agent and reported to transport across the placenta and affect embryonic development. Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to determine the effects of TCS exposure on cardiac development. After TCS treatment, the differentiation process was significantly blocked and spontaneous beating rates of CMs were also decreased. Transcriptome analysis showed the dysregulation of genes involved in cardiogenesis, including GATA4 and TNNT2. Additionally, DNA methylation was also altered by TCS exposure, especially in those regions with GATA motif enrichment. These alterations of transcriptome and DNA methylation were all associated with signaling pathways integral to heart development. Our findings indicate that TCS exposure might cause cardiomyocyte differentiation toxicity and provide the new insights into how environmental factors regulate DNA methylation and gene expressions during heart development. |
format | Online Article Text |
id | pubmed-6231387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-62313872018-12-03 Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity Du, Guizhen Yu, Mingming Wang, Lingling Hu, Weiyue Song, Ling Lu, Chuncheng Wang, Xinru Stem Cells Int Research Article Cardiac development is a dynamic process and sensitive to environmental chemicals. Triclosan is widely used as an antibacterial agent and reported to transport across the placenta and affect embryonic development. Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to determine the effects of TCS exposure on cardiac development. After TCS treatment, the differentiation process was significantly blocked and spontaneous beating rates of CMs were also decreased. Transcriptome analysis showed the dysregulation of genes involved in cardiogenesis, including GATA4 and TNNT2. Additionally, DNA methylation was also altered by TCS exposure, especially in those regions with GATA motif enrichment. These alterations of transcriptome and DNA methylation were all associated with signaling pathways integral to heart development. Our findings indicate that TCS exposure might cause cardiomyocyte differentiation toxicity and provide the new insights into how environmental factors regulate DNA methylation and gene expressions during heart development. Hindawi 2018-10-29 /pmc/articles/PMC6231387/ /pubmed/30510588 http://dx.doi.org/10.1155/2018/8608327 Text en Copyright © 2018 Guizhen Du et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Du, Guizhen Yu, Mingming Wang, Lingling Hu, Weiyue Song, Ling Lu, Chuncheng Wang, Xinru Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity |
title | Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity |
title_full | Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity |
title_fullStr | Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity |
title_full_unstemmed | Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity |
title_short | Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity |
title_sort | transcriptome and dna methylome dynamics during triclosan-induced cardiomyocyte differentiation toxicity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231387/ https://www.ncbi.nlm.nih.gov/pubmed/30510588 http://dx.doi.org/10.1155/2018/8608327 |
work_keys_str_mv | AT duguizhen transcriptomeanddnamethylomedynamicsduringtriclosaninducedcardiomyocytedifferentiationtoxicity AT yumingming transcriptomeanddnamethylomedynamicsduringtriclosaninducedcardiomyocytedifferentiationtoxicity AT wanglingling transcriptomeanddnamethylomedynamicsduringtriclosaninducedcardiomyocytedifferentiationtoxicity AT huweiyue transcriptomeanddnamethylomedynamicsduringtriclosaninducedcardiomyocytedifferentiationtoxicity AT songling transcriptomeanddnamethylomedynamicsduringtriclosaninducedcardiomyocytedifferentiationtoxicity AT luchuncheng transcriptomeanddnamethylomedynamicsduringtriclosaninducedcardiomyocytedifferentiationtoxicity AT wangxinru transcriptomeanddnamethylomedynamicsduringtriclosaninducedcardiomyocytedifferentiationtoxicity |