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Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity

Cardiac development is a dynamic process and sensitive to environmental chemicals. Triclosan is widely used as an antibacterial agent and reported to transport across the placenta and affect embryonic development. Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to deter...

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Autores principales: Du, Guizhen, Yu, Mingming, Wang, Lingling, Hu, Weiyue, Song, Ling, Lu, Chuncheng, Wang, Xinru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231387/
https://www.ncbi.nlm.nih.gov/pubmed/30510588
http://dx.doi.org/10.1155/2018/8608327
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author Du, Guizhen
Yu, Mingming
Wang, Lingling
Hu, Weiyue
Song, Ling
Lu, Chuncheng
Wang, Xinru
author_facet Du, Guizhen
Yu, Mingming
Wang, Lingling
Hu, Weiyue
Song, Ling
Lu, Chuncheng
Wang, Xinru
author_sort Du, Guizhen
collection PubMed
description Cardiac development is a dynamic process and sensitive to environmental chemicals. Triclosan is widely used as an antibacterial agent and reported to transport across the placenta and affect embryonic development. Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to determine the effects of TCS exposure on cardiac development. After TCS treatment, the differentiation process was significantly blocked and spontaneous beating rates of CMs were also decreased. Transcriptome analysis showed the dysregulation of genes involved in cardiogenesis, including GATA4 and TNNT2. Additionally, DNA methylation was also altered by TCS exposure, especially in those regions with GATA motif enrichment. These alterations of transcriptome and DNA methylation were all associated with signaling pathways integral to heart development. Our findings indicate that TCS exposure might cause cardiomyocyte differentiation toxicity and provide the new insights into how environmental factors regulate DNA methylation and gene expressions during heart development.
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spelling pubmed-62313872018-12-03 Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity Du, Guizhen Yu, Mingming Wang, Lingling Hu, Weiyue Song, Ling Lu, Chuncheng Wang, Xinru Stem Cells Int Research Article Cardiac development is a dynamic process and sensitive to environmental chemicals. Triclosan is widely used as an antibacterial agent and reported to transport across the placenta and affect embryonic development. Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to determine the effects of TCS exposure on cardiac development. After TCS treatment, the differentiation process was significantly blocked and spontaneous beating rates of CMs were also decreased. Transcriptome analysis showed the dysregulation of genes involved in cardiogenesis, including GATA4 and TNNT2. Additionally, DNA methylation was also altered by TCS exposure, especially in those regions with GATA motif enrichment. These alterations of transcriptome and DNA methylation were all associated with signaling pathways integral to heart development. Our findings indicate that TCS exposure might cause cardiomyocyte differentiation toxicity and provide the new insights into how environmental factors regulate DNA methylation and gene expressions during heart development. Hindawi 2018-10-29 /pmc/articles/PMC6231387/ /pubmed/30510588 http://dx.doi.org/10.1155/2018/8608327 Text en Copyright © 2018 Guizhen Du et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Du, Guizhen
Yu, Mingming
Wang, Lingling
Hu, Weiyue
Song, Ling
Lu, Chuncheng
Wang, Xinru
Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity
title Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity
title_full Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity
title_fullStr Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity
title_full_unstemmed Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity
title_short Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity
title_sort transcriptome and dna methylome dynamics during triclosan-induced cardiomyocyte differentiation toxicity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231387/
https://www.ncbi.nlm.nih.gov/pubmed/30510588
http://dx.doi.org/10.1155/2018/8608327
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