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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

A protocol is presented for generating human induced pluripotent stem cells (hiPSCs) that express endogenous proteins fused to in-frame N- or C-terminal fluorescent tags. The prokaryotic CRISPR/Cas9 system (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) may be used to...

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Detalles Bibliográficos
Autores principales:	Haupt, Amanda, Grancharova, Tanya, Arakaki, Joy, Fuqua, Margaret A., Roberts, Brock, Gunawardane, Ruwanthi N.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2018
Materias:	Genetics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231893/ https://www.ncbi.nlm.nih.gov/pubmed/30199041 http://dx.doi.org/10.3791/58130

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