A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat

A lateral flow immunochromatographic strip test (LFIST) based on a competitive format was developed for rapid and sensitive on-site detection of oseltamivir phosphate (OP) residues in poultry product. The sensitivity (half inhibitory concentration, IC(50)) of the LFIST in the detection of egg and ch...

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Autores principales: Yang, Xingdong, Yang, Jifei, Wang, Yinbiao, Li, Lili, Sun, Zhongke, Yue, Zonghao, Tian, Fengshou, He, Le, Hu, Xiaofei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232097/
https://www.ncbi.nlm.nih.gov/pubmed/30420605
http://dx.doi.org/10.1038/s41598-018-35080-5
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author Yang, Xingdong
Yang, Jifei
Wang, Yinbiao
Li, Lili
Sun, Zhongke
Yue, Zonghao
Tian, Fengshou
He, Le
Hu, Xiaofei
author_facet Yang, Xingdong
Yang, Jifei
Wang, Yinbiao
Li, Lili
Sun, Zhongke
Yue, Zonghao
Tian, Fengshou
He, Le
Hu, Xiaofei
author_sort Yang, Xingdong
collection PubMed
description A lateral flow immunochromatographic strip test (LFIST) based on a competitive format was developed for rapid and sensitive on-site detection of oseltamivir phosphate (OP) residues in poultry product. The sensitivity (half inhibitory concentration, IC(50)) of the LFIST in the detection of egg and chicken meat samples was confirmed to be 2.56 and 2.63 µg/kg, and the limit detection (LOD) value were 0.43 and 0.42 µg/kg, respectively. For intra-assay and inter-assay reproducibility, recoveries of OP spiked samples ranged between 82.8% and 91.2% with coefficients of variations (CV) less than 5.67% (intra-assay) and 6.52% (inter-assay). The performance of LFIST was comparable to high-performance liquid chromatography (HPLC) in a parallel testing of egg samples and chicken samples. LFIST takes less than 5 minutes, eliminates the dependency on professional personnel, and thus can be used as a surveillance tool for on-site detection of OP residues.
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spelling pubmed-62320972018-11-28 A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat Yang, Xingdong Yang, Jifei Wang, Yinbiao Li, Lili Sun, Zhongke Yue, Zonghao Tian, Fengshou He, Le Hu, Xiaofei Sci Rep Article A lateral flow immunochromatographic strip test (LFIST) based on a competitive format was developed for rapid and sensitive on-site detection of oseltamivir phosphate (OP) residues in poultry product. The sensitivity (half inhibitory concentration, IC(50)) of the LFIST in the detection of egg and chicken meat samples was confirmed to be 2.56 and 2.63 µg/kg, and the limit detection (LOD) value were 0.43 and 0.42 µg/kg, respectively. For intra-assay and inter-assay reproducibility, recoveries of OP spiked samples ranged between 82.8% and 91.2% with coefficients of variations (CV) less than 5.67% (intra-assay) and 6.52% (inter-assay). The performance of LFIST was comparable to high-performance liquid chromatography (HPLC) in a parallel testing of egg samples and chicken samples. LFIST takes less than 5 minutes, eliminates the dependency on professional personnel, and thus can be used as a surveillance tool for on-site detection of OP residues. Nature Publishing Group UK 2018-11-12 /pmc/articles/PMC6232097/ /pubmed/30420605 http://dx.doi.org/10.1038/s41598-018-35080-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Xingdong
Yang, Jifei
Wang, Yinbiao
Li, Lili
Sun, Zhongke
Yue, Zonghao
Tian, Fengshou
He, Le
Hu, Xiaofei
A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat
title A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat
title_full A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat
title_fullStr A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat
title_full_unstemmed A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat
title_short A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat
title_sort lateral flow immunochromato-graphic strip test for rapid detection of oseltamivir phosphate in egg and chicken meat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232097/
https://www.ncbi.nlm.nih.gov/pubmed/30420605
http://dx.doi.org/10.1038/s41598-018-35080-5
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