Cargando…

Structural insights into the function of type VI secretion system TssA subunits

The type VI secretion system (T6SS) is a multi-protein complex that injects bacterial effector proteins into target cells. It is composed of a cell membrane complex anchored to a contractile bacteriophage tail-like apparatus consisting of a sharpened tube that is ejected by the contraction of a shea...

Descripción completa

Detalles Bibliográficos
Autores principales: Dix, Samuel R., Owen, Hayley J., Sun, Ruyue, Ahmad, Asma, Shastri, Sravanthi, Spiewak, Helena L., Mosby, Daniel J., Harris, Matthew J., Batters, Sarah L., Brooker, Thomas A., Tzokov, Svetomir B., Sedelnikova, Svetlana E., Baker, Patrick J., Bullough, Per A., Rice, David W., Thomas, Mark S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232143/
https://www.ncbi.nlm.nih.gov/pubmed/30420757
http://dx.doi.org/10.1038/s41467-018-07247-1
_version_ 1783370350228144128
author Dix, Samuel R.
Owen, Hayley J.
Sun, Ruyue
Ahmad, Asma
Shastri, Sravanthi
Spiewak, Helena L.
Mosby, Daniel J.
Harris, Matthew J.
Batters, Sarah L.
Brooker, Thomas A.
Tzokov, Svetomir B.
Sedelnikova, Svetlana E.
Baker, Patrick J.
Bullough, Per A.
Rice, David W.
Thomas, Mark S.
author_facet Dix, Samuel R.
Owen, Hayley J.
Sun, Ruyue
Ahmad, Asma
Shastri, Sravanthi
Spiewak, Helena L.
Mosby, Daniel J.
Harris, Matthew J.
Batters, Sarah L.
Brooker, Thomas A.
Tzokov, Svetomir B.
Sedelnikova, Svetlana E.
Baker, Patrick J.
Bullough, Per A.
Rice, David W.
Thomas, Mark S.
author_sort Dix, Samuel R.
collection PubMed
description The type VI secretion system (T6SS) is a multi-protein complex that injects bacterial effector proteins into target cells. It is composed of a cell membrane complex anchored to a contractile bacteriophage tail-like apparatus consisting of a sharpened tube that is ejected by the contraction of a sheath against a baseplate. We present structural and biochemical studies on TssA subunits from two different T6SSs that reveal radically different quaternary structures in comparison to the dodecameric E. coli TssA that arise from differences in their C-terminal sequences. Despite this, the different TssAs retain equivalent interactions with other components of the complex and position their highly conserved N-terminal ImpA_N domain at the same radius from the centre of the sheath as a result of their distinct domain architectures, which includes additional spacer domains and highly mobile interdomain linkers. Together, these variations allow these distinct TssAs to perform a similar function in the complex.
format Online
Article
Text
id pubmed-6232143
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-62321432018-11-14 Structural insights into the function of type VI secretion system TssA subunits Dix, Samuel R. Owen, Hayley J. Sun, Ruyue Ahmad, Asma Shastri, Sravanthi Spiewak, Helena L. Mosby, Daniel J. Harris, Matthew J. Batters, Sarah L. Brooker, Thomas A. Tzokov, Svetomir B. Sedelnikova, Svetlana E. Baker, Patrick J. Bullough, Per A. Rice, David W. Thomas, Mark S. Nat Commun Article The type VI secretion system (T6SS) is a multi-protein complex that injects bacterial effector proteins into target cells. It is composed of a cell membrane complex anchored to a contractile bacteriophage tail-like apparatus consisting of a sharpened tube that is ejected by the contraction of a sheath against a baseplate. We present structural and biochemical studies on TssA subunits from two different T6SSs that reveal radically different quaternary structures in comparison to the dodecameric E. coli TssA that arise from differences in their C-terminal sequences. Despite this, the different TssAs retain equivalent interactions with other components of the complex and position their highly conserved N-terminal ImpA_N domain at the same radius from the centre of the sheath as a result of their distinct domain architectures, which includes additional spacer domains and highly mobile interdomain linkers. Together, these variations allow these distinct TssAs to perform a similar function in the complex. Nature Publishing Group UK 2018-11-12 /pmc/articles/PMC6232143/ /pubmed/30420757 http://dx.doi.org/10.1038/s41467-018-07247-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dix, Samuel R.
Owen, Hayley J.
Sun, Ruyue
Ahmad, Asma
Shastri, Sravanthi
Spiewak, Helena L.
Mosby, Daniel J.
Harris, Matthew J.
Batters, Sarah L.
Brooker, Thomas A.
Tzokov, Svetomir B.
Sedelnikova, Svetlana E.
Baker, Patrick J.
Bullough, Per A.
Rice, David W.
Thomas, Mark S.
Structural insights into the function of type VI secretion system TssA subunits
title Structural insights into the function of type VI secretion system TssA subunits
title_full Structural insights into the function of type VI secretion system TssA subunits
title_fullStr Structural insights into the function of type VI secretion system TssA subunits
title_full_unstemmed Structural insights into the function of type VI secretion system TssA subunits
title_short Structural insights into the function of type VI secretion system TssA subunits
title_sort structural insights into the function of type vi secretion system tssa subunits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232143/
https://www.ncbi.nlm.nih.gov/pubmed/30420757
http://dx.doi.org/10.1038/s41467-018-07247-1
work_keys_str_mv AT dixsamuelr structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT owenhayleyj structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT sunruyue structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT ahmadasma structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT shastrisravanthi structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT spiewakhelenal structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT mosbydanielj structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT harrismatthewj structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT batterssarahl structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT brookerthomasa structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT tzokovsvetomirb structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT sedelnikovasvetlanae structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT bakerpatrickj structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT bulloughpera structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT ricedavidw structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits
AT thomasmarks structuralinsightsintothefunctionoftypevisecretionsystemtssasubunits