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Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract

Siwei Jianghuang Decoction Powder (SWJH) documented originally in the Four Medical Tantras-Blue Glaze exhibited beneficial effects on diabetic nephropathy (DN) via combined synergistically action of multiple formula components including Curcumae longae Rhizoma, Berberidis dictyophyllae Cortex, Phyll...

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Autores principales: Lai, Xianrong, Tong, Dong, Ai, Xiaopeng, Wu, Jiasi, Luo, Yu, Zuo, Fang, Wei, Zhicheng, Li, Yanqiao, Huang, Wanyi, Wang, Wenqian, Jiang, Qing, Meng, Xianli, Zeng, Yong, Wang, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232159/
https://www.ncbi.nlm.nih.gov/pubmed/30420600
http://dx.doi.org/10.1038/s41598-018-35148-2
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author Lai, Xianrong
Tong, Dong
Ai, Xiaopeng
Wu, Jiasi
Luo, Yu
Zuo, Fang
Wei, Zhicheng
Li, Yanqiao
Huang, Wanyi
Wang, Wenqian
Jiang, Qing
Meng, Xianli
Zeng, Yong
Wang, Ping
author_facet Lai, Xianrong
Tong, Dong
Ai, Xiaopeng
Wu, Jiasi
Luo, Yu
Zuo, Fang
Wei, Zhicheng
Li, Yanqiao
Huang, Wanyi
Wang, Wenqian
Jiang, Qing
Meng, Xianli
Zeng, Yong
Wang, Ping
author_sort Lai, Xianrong
collection PubMed
description Siwei Jianghuang Decoction Powder (SWJH) documented originally in the Four Medical Tantras-Blue Glaze exhibited beneficial effects on diabetic nephropathy (DN) via combined synergistically action of multiple formula components including Curcumae longae Rhizoma, Berberidis dictyophyllae Cortex, Phyllanthi Fructus and Tribuli Fructus. This study investigated the effects of SWJH on DN in db/db mice and possible underlying mechanisms. The ten weeks old db/db mice treated with SWJH by intra-gastric administration once a day for 8 weeks. After 8 weeks, body weight, water and food intake of mice were recorded. The level of fasting blood glucose (FBG) was measured. Serum creatinine (Scr), blood urea nitrogen (BUN), urine microalbumin (UMAlb), serum uric acid (UA) and urinary albumin excretion (UAE) were detected. An enzyme-linked immunosorbent assay was performed to test serum vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). Real-time PCR and Western blot analysis were used to test mRNA and protein expression of hypoxia inducible factor-1α (HIF-1α), VEGF and TGF-β1 in kidney tissue. SWJH treatment significantly reduced the levels of FBG, Scr, BUN, UMAlb, UA and UAE and retarded renal fibrosis. SWJH treatment further significantly reduced serum TGF-β1 level and downregulated the expression of HIF-1α, VEGF and TGF-β1 at both mRNA and protein levels. Principal component analysis and partial least squares regression and hierarchical cluster analysis demonstrated that SWJH treatment significantly ameliorated renal damage in DN mice. These consequences suggested that SWJH formulations were effective in the treatment of DN through regulating the HIF-1α, VEGF and TGF-β1 overexpression.
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spelling pubmed-62321592018-11-28 Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract Lai, Xianrong Tong, Dong Ai, Xiaopeng Wu, Jiasi Luo, Yu Zuo, Fang Wei, Zhicheng Li, Yanqiao Huang, Wanyi Wang, Wenqian Jiang, Qing Meng, Xianli Zeng, Yong Wang, Ping Sci Rep Article Siwei Jianghuang Decoction Powder (SWJH) documented originally in the Four Medical Tantras-Blue Glaze exhibited beneficial effects on diabetic nephropathy (DN) via combined synergistically action of multiple formula components including Curcumae longae Rhizoma, Berberidis dictyophyllae Cortex, Phyllanthi Fructus and Tribuli Fructus. This study investigated the effects of SWJH on DN in db/db mice and possible underlying mechanisms. The ten weeks old db/db mice treated with SWJH by intra-gastric administration once a day for 8 weeks. After 8 weeks, body weight, water and food intake of mice were recorded. The level of fasting blood glucose (FBG) was measured. Serum creatinine (Scr), blood urea nitrogen (BUN), urine microalbumin (UMAlb), serum uric acid (UA) and urinary albumin excretion (UAE) were detected. An enzyme-linked immunosorbent assay was performed to test serum vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). Real-time PCR and Western blot analysis were used to test mRNA and protein expression of hypoxia inducible factor-1α (HIF-1α), VEGF and TGF-β1 in kidney tissue. SWJH treatment significantly reduced the levels of FBG, Scr, BUN, UMAlb, UA and UAE and retarded renal fibrosis. SWJH treatment further significantly reduced serum TGF-β1 level and downregulated the expression of HIF-1α, VEGF and TGF-β1 at both mRNA and protein levels. Principal component analysis and partial least squares regression and hierarchical cluster analysis demonstrated that SWJH treatment significantly ameliorated renal damage in DN mice. These consequences suggested that SWJH formulations were effective in the treatment of DN through regulating the HIF-1α, VEGF and TGF-β1 overexpression. Nature Publishing Group UK 2018-11-12 /pmc/articles/PMC6232159/ /pubmed/30420600 http://dx.doi.org/10.1038/s41598-018-35148-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lai, Xianrong
Tong, Dong
Ai, Xiaopeng
Wu, Jiasi
Luo, Yu
Zuo, Fang
Wei, Zhicheng
Li, Yanqiao
Huang, Wanyi
Wang, Wenqian
Jiang, Qing
Meng, Xianli
Zeng, Yong
Wang, Ping
Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract
title Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract
title_full Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract
title_fullStr Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract
title_full_unstemmed Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract
title_short Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract
title_sort amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula siwei jianghuang decoction powder extract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232159/
https://www.ncbi.nlm.nih.gov/pubmed/30420600
http://dx.doi.org/10.1038/s41598-018-35148-2
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