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Carotenoid Accumulation and Its Contribution to Flower Coloration of Osmanthus fragrans
Among naturally occurring pigments, carotenoids are importantly involved in the photosynthesis of plants and responsible for the coloration of petals and fruits. Osmanthus fragrans Lour., a famous ornamental plant, has many cultivars with different flower color. Petal coloration in O. fragrans mainl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232703/ https://www.ncbi.nlm.nih.gov/pubmed/30459779 http://dx.doi.org/10.3389/fpls.2018.01499 |
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author | Wang, Yiguang Zhang, Chao Dong, Bin Fu, Jianxin Hu, Shaoqing Zhao, Hongbo |
author_facet | Wang, Yiguang Zhang, Chao Dong, Bin Fu, Jianxin Hu, Shaoqing Zhao, Hongbo |
author_sort | Wang, Yiguang |
collection | PubMed |
description | Among naturally occurring pigments, carotenoids are importantly involved in the photosynthesis of plants and responsible for the coloration of petals and fruits. Osmanthus fragrans Lour., a famous ornamental plant, has many cultivars with different flower color. Petal coloration in O. fragrans mainly depends on the kinds of carotenoids and their contents. To investigate the mechanism of flower coloration in different cultivars, an analysis of phenotypic classification, phytochemistry, as well as the expression of carotenoid metabolism genes based on different groups was performed in the present study. Two main clusters including the orange-red cluster containing Aurantiacus cultivars and the yellowish-white cluster containing the other three cultivar groups were classified using the CIEL(∗)a(∗)b(∗) system. No significant differences in flavonoid contents were observed between these two clusters. However, carotenoids, especially α-carotene and β-carotene, were found to have crucial roles in the diversity of floral coloration among the different cultivars. Carotenoid compositions in the petals of cultivars from both clusters consisted of α-carotene, β-carotene, α-cryptoxanthin, β-cryptoxanthin, lutein, and zeaxanthin, but carotenoid accumulation patterns during the flowering process were different. The petals of the yellowish-white cultivars exhibited high contents of β-carotene, lutein and α-carotene, whereas the petals of the orange-red cultivars mainly contained β-carotene and α-carotene. The profound diversity in the total carotenoid concentrations in the two clusters was determined by the transcript levels of OfCCD4. Furthermore, the accumulation of upstream products with orange color in orange-red cultivars was partially due to the low expression of OfCHYB, whereas the relatively higher OfCHYB expression in the petals of the yellowish-white cultivars led to higher proportions of lutein, which is yellow. We also found that downregulation of OfLCYE, which encodes 𝜀-ring cyclase, indicated that the carotenoid flux of most cultivars mainly resulted in more β, β-branched products. Additionally, carotenoid biosynthesis in green tissues and petals was compared, revealing the tissue specificity of carotenoid accumulation in O. fragrans. Therefore, the effects of multiple genes on carotenoid accumulation give rise to the colorful O. fragrans. |
format | Online Article Text |
id | pubmed-6232703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62327032018-11-20 Carotenoid Accumulation and Its Contribution to Flower Coloration of Osmanthus fragrans Wang, Yiguang Zhang, Chao Dong, Bin Fu, Jianxin Hu, Shaoqing Zhao, Hongbo Front Plant Sci Plant Science Among naturally occurring pigments, carotenoids are importantly involved in the photosynthesis of plants and responsible for the coloration of petals and fruits. Osmanthus fragrans Lour., a famous ornamental plant, has many cultivars with different flower color. Petal coloration in O. fragrans mainly depends on the kinds of carotenoids and their contents. To investigate the mechanism of flower coloration in different cultivars, an analysis of phenotypic classification, phytochemistry, as well as the expression of carotenoid metabolism genes based on different groups was performed in the present study. Two main clusters including the orange-red cluster containing Aurantiacus cultivars and the yellowish-white cluster containing the other three cultivar groups were classified using the CIEL(∗)a(∗)b(∗) system. No significant differences in flavonoid contents were observed between these two clusters. However, carotenoids, especially α-carotene and β-carotene, were found to have crucial roles in the diversity of floral coloration among the different cultivars. Carotenoid compositions in the petals of cultivars from both clusters consisted of α-carotene, β-carotene, α-cryptoxanthin, β-cryptoxanthin, lutein, and zeaxanthin, but carotenoid accumulation patterns during the flowering process were different. The petals of the yellowish-white cultivars exhibited high contents of β-carotene, lutein and α-carotene, whereas the petals of the orange-red cultivars mainly contained β-carotene and α-carotene. The profound diversity in the total carotenoid concentrations in the two clusters was determined by the transcript levels of OfCCD4. Furthermore, the accumulation of upstream products with orange color in orange-red cultivars was partially due to the low expression of OfCHYB, whereas the relatively higher OfCHYB expression in the petals of the yellowish-white cultivars led to higher proportions of lutein, which is yellow. We also found that downregulation of OfLCYE, which encodes 𝜀-ring cyclase, indicated that the carotenoid flux of most cultivars mainly resulted in more β, β-branched products. Additionally, carotenoid biosynthesis in green tissues and petals was compared, revealing the tissue specificity of carotenoid accumulation in O. fragrans. Therefore, the effects of multiple genes on carotenoid accumulation give rise to the colorful O. fragrans. Frontiers Media S.A. 2018-10-16 /pmc/articles/PMC6232703/ /pubmed/30459779 http://dx.doi.org/10.3389/fpls.2018.01499 Text en Copyright © 2018 Wang, Zhang, Dong, Fu, Hu and Zhao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Wang, Yiguang Zhang, Chao Dong, Bin Fu, Jianxin Hu, Shaoqing Zhao, Hongbo Carotenoid Accumulation and Its Contribution to Flower Coloration of Osmanthus fragrans |
title | Carotenoid Accumulation and Its Contribution to Flower Coloration of Osmanthus fragrans |
title_full | Carotenoid Accumulation and Its Contribution to Flower Coloration of Osmanthus fragrans |
title_fullStr | Carotenoid Accumulation and Its Contribution to Flower Coloration of Osmanthus fragrans |
title_full_unstemmed | Carotenoid Accumulation and Its Contribution to Flower Coloration of Osmanthus fragrans |
title_short | Carotenoid Accumulation and Its Contribution to Flower Coloration of Osmanthus fragrans |
title_sort | carotenoid accumulation and its contribution to flower coloration of osmanthus fragrans |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232703/ https://www.ncbi.nlm.nih.gov/pubmed/30459779 http://dx.doi.org/10.3389/fpls.2018.01499 |
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