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Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis

Ultrasound has attracted great interest of both industry and scientific communities for its potential use as a physical processing and preservation tool. In this study, Escherichia coli O157:H7 was selected as the model microbe to investigate the ultrasound-induced cell death. Slight variations in m...

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Autores principales: Li, Jiao, Ma, Luyao, Liao, Xinyu, Liu, Donghong, Lu, Xiaonan, Chen, Shiguo, Ye, Xingqian, Ding, Tian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232819/
https://www.ncbi.nlm.nih.gov/pubmed/30459727
http://dx.doi.org/10.3389/fmicb.2018.02486
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author Li, Jiao
Ma, Luyao
Liao, Xinyu
Liu, Donghong
Lu, Xiaonan
Chen, Shiguo
Ye, Xingqian
Ding, Tian
author_facet Li, Jiao
Ma, Luyao
Liao, Xinyu
Liu, Donghong
Lu, Xiaonan
Chen, Shiguo
Ye, Xingqian
Ding, Tian
author_sort Li, Jiao
collection PubMed
description Ultrasound has attracted great interest of both industry and scientific communities for its potential use as a physical processing and preservation tool. In this study, Escherichia coli O157:H7 was selected as the model microbe to investigate the ultrasound-induced cell death. Slight variations in membrane potential and ion exchanges across membrane induced by low-intensity ultrasound increased the membrane permeability of E. coli O157:H7, and this reversible sublethal effect can preserve the viability of E. coli O157:H7 and meanwhile be beneficial for bioprocessing application. In comparison, high-intensity ultrasound resulted in irreversible lethal effect on E. coli O157:H7, which can be applied in the field of microbial inactivation. In addition, both low- and high-intensity ultrasound induced either physical destruction or trigger genetically encoded apoptosis of E. coli O157:H7. Accumulation of reactive oxygen species and decrease of adenosine tri-phosphate might be related to the physiological and biochemical hallmarks of apoptosis, including exposed phosphatidylserine and activated caspases in E. coli O157:H7. The result provides novel insight into the mechanisms of non-thermal physical treatment on the inactivation of bacteria and lays foundation for the further research on the cell signaling and metabolic pathway in apoptotic bacteria.
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spelling pubmed-62328192018-11-20 Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis Li, Jiao Ma, Luyao Liao, Xinyu Liu, Donghong Lu, Xiaonan Chen, Shiguo Ye, Xingqian Ding, Tian Front Microbiol Microbiology Ultrasound has attracted great interest of both industry and scientific communities for its potential use as a physical processing and preservation tool. In this study, Escherichia coli O157:H7 was selected as the model microbe to investigate the ultrasound-induced cell death. Slight variations in membrane potential and ion exchanges across membrane induced by low-intensity ultrasound increased the membrane permeability of E. coli O157:H7, and this reversible sublethal effect can preserve the viability of E. coli O157:H7 and meanwhile be beneficial for bioprocessing application. In comparison, high-intensity ultrasound resulted in irreversible lethal effect on E. coli O157:H7, which can be applied in the field of microbial inactivation. In addition, both low- and high-intensity ultrasound induced either physical destruction or trigger genetically encoded apoptosis of E. coli O157:H7. Accumulation of reactive oxygen species and decrease of adenosine tri-phosphate might be related to the physiological and biochemical hallmarks of apoptosis, including exposed phosphatidylserine and activated caspases in E. coli O157:H7. The result provides novel insight into the mechanisms of non-thermal physical treatment on the inactivation of bacteria and lays foundation for the further research on the cell signaling and metabolic pathway in apoptotic bacteria. Frontiers Media S.A. 2018-10-16 /pmc/articles/PMC6232819/ /pubmed/30459727 http://dx.doi.org/10.3389/fmicb.2018.02486 Text en Copyright © 2018 Li, Ma, Liao, Liu, Lu, Chen, Ye and Ding. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Jiao
Ma, Luyao
Liao, Xinyu
Liu, Donghong
Lu, Xiaonan
Chen, Shiguo
Ye, Xingqian
Ding, Tian
Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis
title Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis
title_full Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis
title_fullStr Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis
title_full_unstemmed Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis
title_short Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis
title_sort ultrasound-induced escherichia coli o157:h7 cell death exhibits physical disruption and biochemical apoptosis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232819/
https://www.ncbi.nlm.nih.gov/pubmed/30459727
http://dx.doi.org/10.3389/fmicb.2018.02486
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