Cargando…
Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells
Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the endoplasmic reticulum (ER) but also to lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and fu...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232964/ https://www.ncbi.nlm.nih.gov/pubmed/29949452 http://dx.doi.org/10.1091/mbc.E18-05-0282 |
_version_ | 1783370495472697344 |
---|---|
author | Jayson, Christina B. K. Arlt, Henning Fischer, Alexander W. Lai, Zon Weng Farese, Robert V. Walther, Tobias C. |
author_facet | Jayson, Christina B. K. Arlt, Henning Fischer, Alexander W. Lai, Zon Weng Farese, Robert V. Walther, Tobias C. |
author_sort | Jayson, Christina B. K. |
collection | PubMed |
description | Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the endoplasmic reticulum (ER) but also to lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and function in a human mammary carcinoma cell line. Rab18 localized to the ER and to LD membranes on LD induction, with the latter depending on the Rab18 activation state. In cells lacking Rab18, LDs were modestly reduced in size and numbers, but we found little evidence for Rab18 function in LD formation, LD turnover on cell starvation, or the targeting of several proteins to LDs. We conclude that Rab18 is not a general, necessary component of the protein machinery involved in LD biogenesis or turnover. |
format | Online Article Text |
id | pubmed-6232964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-62329642018-11-20 Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells Jayson, Christina B. K. Arlt, Henning Fischer, Alexander W. Lai, Zon Weng Farese, Robert V. Walther, Tobias C. Mol Biol Cell Brief Reports Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the endoplasmic reticulum (ER) but also to lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and function in a human mammary carcinoma cell line. Rab18 localized to the ER and to LD membranes on LD induction, with the latter depending on the Rab18 activation state. In cells lacking Rab18, LDs were modestly reduced in size and numbers, but we found little evidence for Rab18 function in LD formation, LD turnover on cell starvation, or the targeting of several proteins to LDs. We conclude that Rab18 is not a general, necessary component of the protein machinery involved in LD biogenesis or turnover. The American Society for Cell Biology 2018-08-15 /pmc/articles/PMC6232964/ /pubmed/29949452 http://dx.doi.org/10.1091/mbc.E18-05-0282 Text en © 2018 Jayson et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Brief Reports Jayson, Christina B. K. Arlt, Henning Fischer, Alexander W. Lai, Zon Weng Farese, Robert V. Walther, Tobias C. Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells |
title | Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells |
title_full | Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells |
title_fullStr | Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells |
title_full_unstemmed | Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells |
title_short | Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells |
title_sort | rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells |
topic | Brief Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232964/ https://www.ncbi.nlm.nih.gov/pubmed/29949452 http://dx.doi.org/10.1091/mbc.E18-05-0282 |
work_keys_str_mv | AT jaysonchristinabk rab18isnotnecessaryforlipiddropletbiogenesisorturnoverinhumanmammarycarcinomacells AT arlthenning rab18isnotnecessaryforlipiddropletbiogenesisorturnoverinhumanmammarycarcinomacells AT fischeralexanderw rab18isnotnecessaryforlipiddropletbiogenesisorturnoverinhumanmammarycarcinomacells AT laizonweng rab18isnotnecessaryforlipiddropletbiogenesisorturnoverinhumanmammarycarcinomacells AT fareserobertv rab18isnotnecessaryforlipiddropletbiogenesisorturnoverinhumanmammarycarcinomacells AT walthertobiasc rab18isnotnecessaryforlipiddropletbiogenesisorturnoverinhumanmammarycarcinomacells |