Cargando…

Electrochemical Study and Determination of All-trans-Retinol at Carbon Paste Electrode Modified by a Surfactant

The oxidation mechanism of all-trans-retinol (vitamin A(1)) and its several esters in non-aqueous, aqueous organic mixture, and pure aqueous media was investigated by cyclic voltammetry. The oxidation occurred in several irreversible steps. The calculated highest density of electrons in retinoid mol...

Descripción completa

Detalles Bibliográficos
Autores principales: Žabčíková, Simona, Mikysek, Tomáš, Červenka, Libor, Sýs, Milan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: University of Zagreb Faculty of Food Technology and Biotechnology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233017/
https://www.ncbi.nlm.nih.gov/pubmed/30510477
http://dx.doi.org/10.17113/ftb.56.03.18.5618
_version_ 1783370508208701440
author Žabčíková, Simona
Mikysek, Tomáš
Červenka, Libor
Sýs, Milan
author_facet Žabčíková, Simona
Mikysek, Tomáš
Červenka, Libor
Sýs, Milan
author_sort Žabčíková, Simona
collection PubMed
description The oxidation mechanism of all-trans-retinol (vitamin A(1)) and its several esters in non-aqueous, aqueous organic mixture, and pure aqueous media was investigated by cyclic voltammetry. The oxidation occurred in several irreversible steps. The calculated highest density of electrons in retinoid molecules which are delocalized over carbon atoms of the five conjugated double bonds (C5-C14) was found in the part of the molecule involved in oxidation processes. The most sensitive oxidation peak (at +0.8 V vs. Ag/AgCl) was used for development of new direct voltammetric method based on differential pulse voltammetry for the determination of retinol at carbon paste electrode modified with surfactant sodium dodecyl sulfate (CPE/SDS). The results show that 30% (by mass) of modifier SDS exhibited optimal sensitivity and shape of voltammograms. Compared to commonly used glassy carbon electrode (GCE), the CPE/SDS showed significant progress in the retinol electroanalysis. The linear ranges for retinol determination were 1.5·10(−6)–1.8·10(−4) M for CPE/SDS and 4.4·10(−6)–7.0·10(−4) M for GCE with the detection limits of 1.3·10(−6) and 4.6·10(−7) M, respectively.
format Online
Article
Text
id pubmed-6233017
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher University of Zagreb Faculty of Food Technology and Biotechnology
record_format MEDLINE/PubMed
spelling pubmed-62330172018-12-03 Electrochemical Study and Determination of All-trans-Retinol at Carbon Paste Electrode Modified by a Surfactant Žabčíková, Simona Mikysek, Tomáš Červenka, Libor Sýs, Milan Food Technol Biotechnol Original Scientific Papers The oxidation mechanism of all-trans-retinol (vitamin A(1)) and its several esters in non-aqueous, aqueous organic mixture, and pure aqueous media was investigated by cyclic voltammetry. The oxidation occurred in several irreversible steps. The calculated highest density of electrons in retinoid molecules which are delocalized over carbon atoms of the five conjugated double bonds (C5-C14) was found in the part of the molecule involved in oxidation processes. The most sensitive oxidation peak (at +0.8 V vs. Ag/AgCl) was used for development of new direct voltammetric method based on differential pulse voltammetry for the determination of retinol at carbon paste electrode modified with surfactant sodium dodecyl sulfate (CPE/SDS). The results show that 30% (by mass) of modifier SDS exhibited optimal sensitivity and shape of voltammograms. Compared to commonly used glassy carbon electrode (GCE), the CPE/SDS showed significant progress in the retinol electroanalysis. The linear ranges for retinol determination were 1.5·10(−6)–1.8·10(−4) M for CPE/SDS and 4.4·10(−6)–7.0·10(−4) M for GCE with the detection limits of 1.3·10(−6) and 4.6·10(−7) M, respectively. University of Zagreb Faculty of Food Technology and Biotechnology 2018-09 /pmc/articles/PMC6233017/ /pubmed/30510477 http://dx.doi.org/10.17113/ftb.56.03.18.5618 Text en http://creativecommons.org/licenses/by-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution No Derivatives (CC BY-ND) 4.0 License.
spellingShingle Original Scientific Papers
Žabčíková, Simona
Mikysek, Tomáš
Červenka, Libor
Sýs, Milan
Electrochemical Study and Determination of All-trans-Retinol at Carbon Paste Electrode Modified by a Surfactant
title Electrochemical Study and Determination of All-trans-Retinol at Carbon Paste Electrode Modified by a Surfactant
title_full Electrochemical Study and Determination of All-trans-Retinol at Carbon Paste Electrode Modified by a Surfactant
title_fullStr Electrochemical Study and Determination of All-trans-Retinol at Carbon Paste Electrode Modified by a Surfactant
title_full_unstemmed Electrochemical Study and Determination of All-trans-Retinol at Carbon Paste Electrode Modified by a Surfactant
title_short Electrochemical Study and Determination of All-trans-Retinol at Carbon Paste Electrode Modified by a Surfactant
title_sort electrochemical study and determination of all-trans-retinol at carbon paste electrode modified by a surfactant
topic Original Scientific Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233017/
https://www.ncbi.nlm.nih.gov/pubmed/30510477
http://dx.doi.org/10.17113/ftb.56.03.18.5618
work_keys_str_mv AT zabcikovasimona electrochemicalstudyanddeterminationofalltransretinolatcarbonpasteelectrodemodifiedbyasurfactant
AT mikysektomas electrochemicalstudyanddeterminationofalltransretinolatcarbonpasteelectrodemodifiedbyasurfactant
AT cervenkalibor electrochemicalstudyanddeterminationofalltransretinolatcarbonpasteelectrodemodifiedbyasurfactant
AT sysmilan electrochemicalstudyanddeterminationofalltransretinolatcarbonpasteelectrodemodifiedbyasurfactant