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The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria

Mitochondrial gene expression in Saccharomyces cerevisiae is responsible for the production of highly hydrophobic subunits of the oxidative phosphorylation system. Membrane insertion occurs cotranslationally on membrane-bound mitochondrial ribosomes. Here, by employing a systematic mass spectrometry...

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Autores principales: Möller-Hergt, Braulio Vargas, Carlström, Andreas, Stephan, Katharina, Imhof, Axel, Ott, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233058/
https://www.ncbi.nlm.nih.gov/pubmed/30091672
http://dx.doi.org/10.1091/mbc.E18-04-0227
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author Möller-Hergt, Braulio Vargas
Carlström, Andreas
Stephan, Katharina
Imhof, Axel
Ott, Martin
author_facet Möller-Hergt, Braulio Vargas
Carlström, Andreas
Stephan, Katharina
Imhof, Axel
Ott, Martin
author_sort Möller-Hergt, Braulio Vargas
collection PubMed
description Mitochondrial gene expression in Saccharomyces cerevisiae is responsible for the production of highly hydrophobic subunits of the oxidative phosphorylation system. Membrane insertion occurs cotranslationally on membrane-bound mitochondrial ribosomes. Here, by employing a systematic mass spectrometry–based approach, we discovered the previously uncharacterized membrane protein Mrx15 that interacts via a soluble C-terminal domain with the large ribosomal subunit. Mrx15 contacts mitochondrial translation products during their synthesis and plays, together with the ribosome receptor Mba1, an overlapping role in cotranslational protein insertion. Taken together, our data reveal how these ribosome receptors organize membrane protein biogenesis in mitochondria.
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spelling pubmed-62330582018-12-16 The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria Möller-Hergt, Braulio Vargas Carlström, Andreas Stephan, Katharina Imhof, Axel Ott, Martin Mol Biol Cell Articles Mitochondrial gene expression in Saccharomyces cerevisiae is responsible for the production of highly hydrophobic subunits of the oxidative phosphorylation system. Membrane insertion occurs cotranslationally on membrane-bound mitochondrial ribosomes. Here, by employing a systematic mass spectrometry–based approach, we discovered the previously uncharacterized membrane protein Mrx15 that interacts via a soluble C-terminal domain with the large ribosomal subunit. Mrx15 contacts mitochondrial translation products during their synthesis and plays, together with the ribosome receptor Mba1, an overlapping role in cotranslational protein insertion. Taken together, our data reveal how these ribosome receptors organize membrane protein biogenesis in mitochondria. The American Society for Cell Biology 2018-10-01 /pmc/articles/PMC6233058/ /pubmed/30091672 http://dx.doi.org/10.1091/mbc.E18-04-0227 Text en © 2018 Möller-Hergt et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Möller-Hergt, Braulio Vargas
Carlström, Andreas
Stephan, Katharina
Imhof, Axel
Ott, Martin
The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria
title The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria
title_full The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria
title_fullStr The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria
title_full_unstemmed The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria
title_short The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria
title_sort ribosome receptors mrx15 and mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233058/
https://www.ncbi.nlm.nih.gov/pubmed/30091672
http://dx.doi.org/10.1091/mbc.E18-04-0227
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