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The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria
Mitochondrial gene expression in Saccharomyces cerevisiae is responsible for the production of highly hydrophobic subunits of the oxidative phosphorylation system. Membrane insertion occurs cotranslationally on membrane-bound mitochondrial ribosomes. Here, by employing a systematic mass spectrometry...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233058/ https://www.ncbi.nlm.nih.gov/pubmed/30091672 http://dx.doi.org/10.1091/mbc.E18-04-0227 |
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author | Möller-Hergt, Braulio Vargas Carlström, Andreas Stephan, Katharina Imhof, Axel Ott, Martin |
author_facet | Möller-Hergt, Braulio Vargas Carlström, Andreas Stephan, Katharina Imhof, Axel Ott, Martin |
author_sort | Möller-Hergt, Braulio Vargas |
collection | PubMed |
description | Mitochondrial gene expression in Saccharomyces cerevisiae is responsible for the production of highly hydrophobic subunits of the oxidative phosphorylation system. Membrane insertion occurs cotranslationally on membrane-bound mitochondrial ribosomes. Here, by employing a systematic mass spectrometry–based approach, we discovered the previously uncharacterized membrane protein Mrx15 that interacts via a soluble C-terminal domain with the large ribosomal subunit. Mrx15 contacts mitochondrial translation products during their synthesis and plays, together with the ribosome receptor Mba1, an overlapping role in cotranslational protein insertion. Taken together, our data reveal how these ribosome receptors organize membrane protein biogenesis in mitochondria. |
format | Online Article Text |
id | pubmed-6233058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-62330582018-12-16 The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria Möller-Hergt, Braulio Vargas Carlström, Andreas Stephan, Katharina Imhof, Axel Ott, Martin Mol Biol Cell Articles Mitochondrial gene expression in Saccharomyces cerevisiae is responsible for the production of highly hydrophobic subunits of the oxidative phosphorylation system. Membrane insertion occurs cotranslationally on membrane-bound mitochondrial ribosomes. Here, by employing a systematic mass spectrometry–based approach, we discovered the previously uncharacterized membrane protein Mrx15 that interacts via a soluble C-terminal domain with the large ribosomal subunit. Mrx15 contacts mitochondrial translation products during their synthesis and plays, together with the ribosome receptor Mba1, an overlapping role in cotranslational protein insertion. Taken together, our data reveal how these ribosome receptors organize membrane protein biogenesis in mitochondria. The American Society for Cell Biology 2018-10-01 /pmc/articles/PMC6233058/ /pubmed/30091672 http://dx.doi.org/10.1091/mbc.E18-04-0227 Text en © 2018 Möller-Hergt et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Articles Möller-Hergt, Braulio Vargas Carlström, Andreas Stephan, Katharina Imhof, Axel Ott, Martin The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria |
title | The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria |
title_full | The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria |
title_fullStr | The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria |
title_full_unstemmed | The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria |
title_short | The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria |
title_sort | ribosome receptors mrx15 and mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233058/ https://www.ncbi.nlm.nih.gov/pubmed/30091672 http://dx.doi.org/10.1091/mbc.E18-04-0227 |
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