Efficient immunoaffinity chromatography of lymphocytes directly from whole blood

We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protoco...

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Detalles Bibliográficos
Autores principales: Mohr, Fabian, Przibilla, Sabine, Leonhardt, Franziska, Stemberger, Christian, Dreher, Stefan, Müller, Thomas R., Fräßle, Simon P., Schmidt, Georg P., Kiene, Marie-Luise, Stadler, Herbert, Busch, Dirk H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233198/
https://www.ncbi.nlm.nih.gov/pubmed/30425259
http://dx.doi.org/10.1038/s41598-018-34589-z
Descripción
Sumario:We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent functionality of purified cells.

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