Efficient immunoaffinity chromatography of lymphocytes directly from whole blood

We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protoco...

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Autores principales: Mohr, Fabian, Przibilla, Sabine, Leonhardt, Franziska, Stemberger, Christian, Dreher, Stefan, Müller, Thomas R., Fräßle, Simon P., Schmidt, Georg P., Kiene, Marie-Luise, Stadler, Herbert, Busch, Dirk H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233198/
https://www.ncbi.nlm.nih.gov/pubmed/30425259
http://dx.doi.org/10.1038/s41598-018-34589-z
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author Mohr, Fabian
Przibilla, Sabine
Leonhardt, Franziska
Stemberger, Christian
Dreher, Stefan
Müller, Thomas R.
Fräßle, Simon P.
Schmidt, Georg P.
Kiene, Marie-Luise
Stadler, Herbert
Busch, Dirk H.
author_facet Mohr, Fabian
Przibilla, Sabine
Leonhardt, Franziska
Stemberger, Christian
Dreher, Stefan
Müller, Thomas R.
Fräßle, Simon P.
Schmidt, Georg P.
Kiene, Marie-Luise
Stadler, Herbert
Busch, Dirk H.
author_sort Mohr, Fabian
collection PubMed
description We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent functionality of purified cells.
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spelling pubmed-62331982018-11-28 Efficient immunoaffinity chromatography of lymphocytes directly from whole blood Mohr, Fabian Przibilla, Sabine Leonhardt, Franziska Stemberger, Christian Dreher, Stefan Müller, Thomas R. Fräßle, Simon P. Schmidt, Georg P. Kiene, Marie-Luise Stadler, Herbert Busch, Dirk H. Sci Rep Article We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent functionality of purified cells. Nature Publishing Group UK 2018-11-13 /pmc/articles/PMC6233198/ /pubmed/30425259 http://dx.doi.org/10.1038/s41598-018-34589-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mohr, Fabian
Przibilla, Sabine
Leonhardt, Franziska
Stemberger, Christian
Dreher, Stefan
Müller, Thomas R.
Fräßle, Simon P.
Schmidt, Georg P.
Kiene, Marie-Luise
Stadler, Herbert
Busch, Dirk H.
Efficient immunoaffinity chromatography of lymphocytes directly from whole blood
title Efficient immunoaffinity chromatography of lymphocytes directly from whole blood
title_full Efficient immunoaffinity chromatography of lymphocytes directly from whole blood
title_fullStr Efficient immunoaffinity chromatography of lymphocytes directly from whole blood
title_full_unstemmed Efficient immunoaffinity chromatography of lymphocytes directly from whole blood
title_short Efficient immunoaffinity chromatography of lymphocytes directly from whole blood
title_sort efficient immunoaffinity chromatography of lymphocytes directly from whole blood
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233198/
https://www.ncbi.nlm.nih.gov/pubmed/30425259
http://dx.doi.org/10.1038/s41598-018-34589-z
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