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Efficient immunoaffinity chromatography of lymphocytes directly from whole blood
We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protoco...
Autores principales: | Mohr, Fabian, Przibilla, Sabine, Leonhardt, Franziska, Stemberger, Christian, Dreher, Stefan, Müller, Thomas R., Fräßle, Simon P., Schmidt, Georg P., Kiene, Marie-Luise, Stadler, Herbert, Busch, Dirk H. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233198/ https://www.ncbi.nlm.nih.gov/pubmed/30425259 http://dx.doi.org/10.1038/s41598-018-34589-z |
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