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Reinforcement of calcium phosphate cement using alkaline-treated silk fibroin

BACKGROUND: Bone cement plays an important role in the treatment of osteoporotic vertebral compression fractures. Calcium phosphate cement (CPC) is a potential alternative to poly(methyl methacrylate), currently the gold standard of bone cements. However, the poor mechanical properties of CPCs limit...

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Autores principales: Hu, Muli, He, Zhiwei, Han, Fengxuan, Shi, Chen, Zhou, Pinghui, Ling, Feng, Zhu, Xuesong, Yang, Huilin, Li, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233488/
https://www.ncbi.nlm.nih.gov/pubmed/30519015
http://dx.doi.org/10.2147/IJN.S172881
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author Hu, Muli
He, Zhiwei
Han, Fengxuan
Shi, Chen
Zhou, Pinghui
Ling, Feng
Zhu, Xuesong
Yang, Huilin
Li, Bin
author_facet Hu, Muli
He, Zhiwei
Han, Fengxuan
Shi, Chen
Zhou, Pinghui
Ling, Feng
Zhu, Xuesong
Yang, Huilin
Li, Bin
author_sort Hu, Muli
collection PubMed
description BACKGROUND: Bone cement plays an important role in the treatment of osteoporotic vertebral compression fractures. Calcium phosphate cement (CPC) is a potential alternative to poly(methyl methacrylate), currently the gold standard of bone cements. However, the poor mechanical properties of CPCs limit their clinical applications. The objective of this study was to develop reinforced CPCs for minimally invasive orthopedic surgeries by compositing silk fibroin (SF) with α-tricalcium phosphate. METHODS: SF solution was treated with calcium hydroxide and characterized by Zeta potential analyzer and Fourier transform infrared spectroscopy. The alkaline-treated SF (tSF) was com-posited with α-tricalcium phosphate to obtain tSF/CPC composite, which was characterized using mechanical tests, scanning electron microscopy, handling property and biocompatibility tests, and sheep vertebral augmentation tests. RESULTS: Upon treatment with calcium hydroxide, larger SF particles and more abundant negative charge appeared in tSF solution. The tSF/CPCs exhibited a compact structure, which consisted of numerous SF -CPC clusters and needle-like hydroxyapatite (HAp) crystals. In addition, high transition rate of HAp in tSF/CPCs was achieved. As a result, the mechanical property of tSF/ CPC composite cements was enhanced remarkably, with the compressive strength reaching as high as 56.3±1.1 MPa. Moreover, the tSF/CPC cements showed good injectability, anti-washout property, and decent biocompatibility. The tSF/CPCs could be used to augment defected sheep vertebrae to restore their mechanical strength. CONCLUSION: tSF/CPC may be a promising composite bone cement for minimally invasive orthopedic surgeries.
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spelling pubmed-62334882018-12-05 Reinforcement of calcium phosphate cement using alkaline-treated silk fibroin Hu, Muli He, Zhiwei Han, Fengxuan Shi, Chen Zhou, Pinghui Ling, Feng Zhu, Xuesong Yang, Huilin Li, Bin Int J Nanomedicine Original Research BACKGROUND: Bone cement plays an important role in the treatment of osteoporotic vertebral compression fractures. Calcium phosphate cement (CPC) is a potential alternative to poly(methyl methacrylate), currently the gold standard of bone cements. However, the poor mechanical properties of CPCs limit their clinical applications. The objective of this study was to develop reinforced CPCs for minimally invasive orthopedic surgeries by compositing silk fibroin (SF) with α-tricalcium phosphate. METHODS: SF solution was treated with calcium hydroxide and characterized by Zeta potential analyzer and Fourier transform infrared spectroscopy. The alkaline-treated SF (tSF) was com-posited with α-tricalcium phosphate to obtain tSF/CPC composite, which was characterized using mechanical tests, scanning electron microscopy, handling property and biocompatibility tests, and sheep vertebral augmentation tests. RESULTS: Upon treatment with calcium hydroxide, larger SF particles and more abundant negative charge appeared in tSF solution. The tSF/CPCs exhibited a compact structure, which consisted of numerous SF -CPC clusters and needle-like hydroxyapatite (HAp) crystals. In addition, high transition rate of HAp in tSF/CPCs was achieved. As a result, the mechanical property of tSF/ CPC composite cements was enhanced remarkably, with the compressive strength reaching as high as 56.3±1.1 MPa. Moreover, the tSF/CPC cements showed good injectability, anti-washout property, and decent biocompatibility. The tSF/CPCs could be used to augment defected sheep vertebrae to restore their mechanical strength. CONCLUSION: tSF/CPC may be a promising composite bone cement for minimally invasive orthopedic surgeries. Dove Medical Press 2018-11-09 /pmc/articles/PMC6233488/ /pubmed/30519015 http://dx.doi.org/10.2147/IJN.S172881 Text en © 2018 Hu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Hu, Muli
He, Zhiwei
Han, Fengxuan
Shi, Chen
Zhou, Pinghui
Ling, Feng
Zhu, Xuesong
Yang, Huilin
Li, Bin
Reinforcement of calcium phosphate cement using alkaline-treated silk fibroin
title Reinforcement of calcium phosphate cement using alkaline-treated silk fibroin
title_full Reinforcement of calcium phosphate cement using alkaline-treated silk fibroin
title_fullStr Reinforcement of calcium phosphate cement using alkaline-treated silk fibroin
title_full_unstemmed Reinforcement of calcium phosphate cement using alkaline-treated silk fibroin
title_short Reinforcement of calcium phosphate cement using alkaline-treated silk fibroin
title_sort reinforcement of calcium phosphate cement using alkaline-treated silk fibroin
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233488/
https://www.ncbi.nlm.nih.gov/pubmed/30519015
http://dx.doi.org/10.2147/IJN.S172881
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