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Genetic diversity of Bletilla striata assessed by SCoT and IRAP markers

BACKGROUND: Bletilla striata is a well-known traditional Chinese herb with varieties of functions. In China, the natural resources of Bletilla striata have been severely damaged because of the excessive exploitation and destruction of natural habitats. The aim of present study was to provide a refer...

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Autores principales: Guo, Yan, Zhai, Lina, Long, Hao, Chen, Nipi, Gao, Chengxian, Ding, Zhishan, Jin, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233545/
https://www.ncbi.nlm.nih.gov/pubmed/30459533
http://dx.doi.org/10.1186/s41065-018-0074-4
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author Guo, Yan
Zhai, Lina
Long, Hao
Chen, Nipi
Gao, Chengxian
Ding, Zhishan
Jin, Bo
author_facet Guo, Yan
Zhai, Lina
Long, Hao
Chen, Nipi
Gao, Chengxian
Ding, Zhishan
Jin, Bo
author_sort Guo, Yan
collection PubMed
description BACKGROUND: Bletilla striata is a well-known traditional Chinese herb with varieties of functions. In China, the natural resources of Bletilla striata have been severely damaged because of the excessive exploitation and destruction of natural habitats. The aim of present study was to provide a reference for fully exploiting and utilizing the germplasm resources of Bletilla striata. RESULTS: The genetic diversity of 50 varieties of Bletilla striata from different area in China was analyzed by SCoT and IRAP molecular marker technique. A total of 209 bands were amplified by 20 groups of SCoT primers, of which 201 (96.17%) were polymorphic, and 47 polymorphic bands (94%) were observed in 50 bands amplified by 8 groups of IRAP primers. The 50 populations of Bletilla striata were divided into two major groups by SCoT and IRAP at the genetic similarity coefficient value of 0.60 and 0.68 individually. The partition of clusters in the unweighted pair group method with arithmetic mean dendrogram and principal coordinate analysis plot based on the SCoT and IRAP markers was similar. CONCLUSIONS: Results indicated the abundant genetic diversity of Bletilla striata among different areas. Our results will provide useful information for resource protection.
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spelling pubmed-62335452018-11-20 Genetic diversity of Bletilla striata assessed by SCoT and IRAP markers Guo, Yan Zhai, Lina Long, Hao Chen, Nipi Gao, Chengxian Ding, Zhishan Jin, Bo Hereditas Research BACKGROUND: Bletilla striata is a well-known traditional Chinese herb with varieties of functions. In China, the natural resources of Bletilla striata have been severely damaged because of the excessive exploitation and destruction of natural habitats. The aim of present study was to provide a reference for fully exploiting and utilizing the germplasm resources of Bletilla striata. RESULTS: The genetic diversity of 50 varieties of Bletilla striata from different area in China was analyzed by SCoT and IRAP molecular marker technique. A total of 209 bands were amplified by 20 groups of SCoT primers, of which 201 (96.17%) were polymorphic, and 47 polymorphic bands (94%) were observed in 50 bands amplified by 8 groups of IRAP primers. The 50 populations of Bletilla striata were divided into two major groups by SCoT and IRAP at the genetic similarity coefficient value of 0.60 and 0.68 individually. The partition of clusters in the unweighted pair group method with arithmetic mean dendrogram and principal coordinate analysis plot based on the SCoT and IRAP markers was similar. CONCLUSIONS: Results indicated the abundant genetic diversity of Bletilla striata among different areas. Our results will provide useful information for resource protection. BioMed Central 2018-11-12 /pmc/articles/PMC6233545/ /pubmed/30459533 http://dx.doi.org/10.1186/s41065-018-0074-4 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Guo, Yan
Zhai, Lina
Long, Hao
Chen, Nipi
Gao, Chengxian
Ding, Zhishan
Jin, Bo
Genetic diversity of Bletilla striata assessed by SCoT and IRAP markers
title Genetic diversity of Bletilla striata assessed by SCoT and IRAP markers
title_full Genetic diversity of Bletilla striata assessed by SCoT and IRAP markers
title_fullStr Genetic diversity of Bletilla striata assessed by SCoT and IRAP markers
title_full_unstemmed Genetic diversity of Bletilla striata assessed by SCoT and IRAP markers
title_short Genetic diversity of Bletilla striata assessed by SCoT and IRAP markers
title_sort genetic diversity of bletilla striata assessed by scot and irap markers
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233545/
https://www.ncbi.nlm.nih.gov/pubmed/30459533
http://dx.doi.org/10.1186/s41065-018-0074-4
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