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Genetic architecture of common bunt resistance in winter wheat using genome-wide association study

BACKGROUND: Common bunt (caused by Tilletia caries and T. foetida) has been considered as a major disease in wheat (Triticum aestivum) following rust (Puccinia spp.) in the Near East and is economically important in the Great Plains, USA. Despite the fact that it can be easily controlled using seed...

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Autores principales: Mourad, Amira M. I., Sallam, Ahmed, Belamkar, Vikas, Mahdy, Ezzat, Bakheit, Bahy, Abo El-Wafaa, Atif, Stephen Baenziger, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6234641/
https://www.ncbi.nlm.nih.gov/pubmed/30424724
http://dx.doi.org/10.1186/s12870-018-1435-x
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author Mourad, Amira M. I.
Sallam, Ahmed
Belamkar, Vikas
Mahdy, Ezzat
Bakheit, Bahy
Abo El-Wafaa, Atif
Stephen Baenziger, P.
author_facet Mourad, Amira M. I.
Sallam, Ahmed
Belamkar, Vikas
Mahdy, Ezzat
Bakheit, Bahy
Abo El-Wafaa, Atif
Stephen Baenziger, P.
author_sort Mourad, Amira M. I.
collection PubMed
description BACKGROUND: Common bunt (caused by Tilletia caries and T. foetida) has been considered as a major disease in wheat (Triticum aestivum) following rust (Puccinia spp.) in the Near East and is economically important in the Great Plains, USA. Despite the fact that it can be easily controlled using seed treatment with fungicides, fungicides often cannot or may not be used in organic and low-input fields. Planting common bunt resistant genotypes is an alternative. RESULTS: To identify resistance genes for Nebraska common bunt race, the global set of differential lines were inoculated. Nine differential lines carrying nine different genes had 0% infected heads and seemed to be resistant to Nebraska race. To understand the genetic basis of the resistance in Nebraska winter wheat, a set of 330 genotypes were inoculated and evaluated under field conditions in two locations. Out of the 330 genotypes, 62 genotypes had different degrees of resistance. Moreover, plant height, chlorophyll content and days to heading were scored in both locations. Using genome-wide association study, 123 SNPs located on fourteen chromosomes were identified to be associated with the resistance. Different degrees of linkage disequilibrium was found among the significant SNPs and they explained 1.00 to 9.00% of the phenotypic variance, indicating the presence of many minor QTLs controlling the resistance. CONCLUSION: Based on the chromosomal location of some of the known genes, some SNPs may be associated with Bt1, Bt6, Bt11 and Bt12 resistance loci. The remaining significant SNPs may be novel alleles that were not reported previously. Common bunt resistance seems to be an independent trait as no correlation was found between a number of infected heads and chlorophyll content, days to heading or plant height. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1435-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-62346412018-11-23 Genetic architecture of common bunt resistance in winter wheat using genome-wide association study Mourad, Amira M. I. Sallam, Ahmed Belamkar, Vikas Mahdy, Ezzat Bakheit, Bahy Abo El-Wafaa, Atif Stephen Baenziger, P. BMC Plant Biol Research Article BACKGROUND: Common bunt (caused by Tilletia caries and T. foetida) has been considered as a major disease in wheat (Triticum aestivum) following rust (Puccinia spp.) in the Near East and is economically important in the Great Plains, USA. Despite the fact that it can be easily controlled using seed treatment with fungicides, fungicides often cannot or may not be used in organic and low-input fields. Planting common bunt resistant genotypes is an alternative. RESULTS: To identify resistance genes for Nebraska common bunt race, the global set of differential lines were inoculated. Nine differential lines carrying nine different genes had 0% infected heads and seemed to be resistant to Nebraska race. To understand the genetic basis of the resistance in Nebraska winter wheat, a set of 330 genotypes were inoculated and evaluated under field conditions in two locations. Out of the 330 genotypes, 62 genotypes had different degrees of resistance. Moreover, plant height, chlorophyll content and days to heading were scored in both locations. Using genome-wide association study, 123 SNPs located on fourteen chromosomes were identified to be associated with the resistance. Different degrees of linkage disequilibrium was found among the significant SNPs and they explained 1.00 to 9.00% of the phenotypic variance, indicating the presence of many minor QTLs controlling the resistance. CONCLUSION: Based on the chromosomal location of some of the known genes, some SNPs may be associated with Bt1, Bt6, Bt11 and Bt12 resistance loci. The remaining significant SNPs may be novel alleles that were not reported previously. Common bunt resistance seems to be an independent trait as no correlation was found between a number of infected heads and chlorophyll content, days to heading or plant height. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1435-x) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-13 /pmc/articles/PMC6234641/ /pubmed/30424724 http://dx.doi.org/10.1186/s12870-018-1435-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Mourad, Amira M. I.
Sallam, Ahmed
Belamkar, Vikas
Mahdy, Ezzat
Bakheit, Bahy
Abo El-Wafaa, Atif
Stephen Baenziger, P.
Genetic architecture of common bunt resistance in winter wheat using genome-wide association study
title Genetic architecture of common bunt resistance in winter wheat using genome-wide association study
title_full Genetic architecture of common bunt resistance in winter wheat using genome-wide association study
title_fullStr Genetic architecture of common bunt resistance in winter wheat using genome-wide association study
title_full_unstemmed Genetic architecture of common bunt resistance in winter wheat using genome-wide association study
title_short Genetic architecture of common bunt resistance in winter wheat using genome-wide association study
title_sort genetic architecture of common bunt resistance in winter wheat using genome-wide association study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6234641/
https://www.ncbi.nlm.nih.gov/pubmed/30424724
http://dx.doi.org/10.1186/s12870-018-1435-x
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