Cargando…

Drivers and Dynamics of Methicillin-Resistant Livestock-Associated Staphylococcus aureus CC398 in Pigs and Humans in Denmark

The spread of livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398) within the Danish pig production system has been linked to an increased number of human infections. Yet, the population structure and transmission dynamics of this important pathogen rem...

Descripción completa

Detalles Bibliográficos
Autores principales: Sieber, Raphael N., Skov, Robert L., Nielsen, Jens, Schulz, Jana, Price, Lance B., Aarestrup, Frank M., Larsen, Anders R., Stegger, Marc, Larsen, Jesper
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6234867/
https://www.ncbi.nlm.nih.gov/pubmed/30425152
http://dx.doi.org/10.1128/mBio.02142-18
_version_ 1783370792210268160
author Sieber, Raphael N.
Skov, Robert L.
Nielsen, Jens
Schulz, Jana
Price, Lance B.
Aarestrup, Frank M.
Larsen, Anders R.
Stegger, Marc
Larsen, Jesper
author_facet Sieber, Raphael N.
Skov, Robert L.
Nielsen, Jens
Schulz, Jana
Price, Lance B.
Aarestrup, Frank M.
Larsen, Anders R.
Stegger, Marc
Larsen, Jesper
author_sort Sieber, Raphael N.
collection PubMed
description The spread of livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398) within the Danish pig production system has been linked to an increased number of human infections. Yet, the population structure and transmission dynamics of this important pathogen remain poorly understood. In this study, whole-genome sequences from 371 LA-MRSA CC398 isolates collected between 2004 and 2015 were subjected to bioinformatic analyses. The isolates originated from Danish pig farms (n = 209) and people having livestock contact (n = 79). In addition, whole-genome sequence data from 82 isolates representing an international reference collection and 83 isolates from Danish patients were included in the analysis. The results demonstrated that the increasing prevalence of LA-MRSA CC398 in Danish pigs and patients was caused by clonal expansion of three dominant lineages. The results also showed that these lineages were enriched for the tetracycline resistance gene tet(K) and other determinants conferring resistance to some of the most frequently used antimicrobials in Danish pigs. The association between pig movements and the spread of LA-MRSA CC398 was assessed in a Poisson regression analysis of 17,009 pig movements into 273 farms with known LA-MRSA CC398 status. The results demonstrated that animal movements have played a critical role in the dissemination of LA-MRSA CC398 within the Danish pig production system, although other transmission routes may also have contributed. Consistent with this scenario, the genetic relatedness of isolates from different farms was positively correlated with the number of animal movements between the farms.
format Online
Article
Text
id pubmed-6234867
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-62348672018-11-15 Drivers and Dynamics of Methicillin-Resistant Livestock-Associated Staphylococcus aureus CC398 in Pigs and Humans in Denmark Sieber, Raphael N. Skov, Robert L. Nielsen, Jens Schulz, Jana Price, Lance B. Aarestrup, Frank M. Larsen, Anders R. Stegger, Marc Larsen, Jesper mBio Research Article The spread of livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398) within the Danish pig production system has been linked to an increased number of human infections. Yet, the population structure and transmission dynamics of this important pathogen remain poorly understood. In this study, whole-genome sequences from 371 LA-MRSA CC398 isolates collected between 2004 and 2015 were subjected to bioinformatic analyses. The isolates originated from Danish pig farms (n = 209) and people having livestock contact (n = 79). In addition, whole-genome sequence data from 82 isolates representing an international reference collection and 83 isolates from Danish patients were included in the analysis. The results demonstrated that the increasing prevalence of LA-MRSA CC398 in Danish pigs and patients was caused by clonal expansion of three dominant lineages. The results also showed that these lineages were enriched for the tetracycline resistance gene tet(K) and other determinants conferring resistance to some of the most frequently used antimicrobials in Danish pigs. The association between pig movements and the spread of LA-MRSA CC398 was assessed in a Poisson regression analysis of 17,009 pig movements into 273 farms with known LA-MRSA CC398 status. The results demonstrated that animal movements have played a critical role in the dissemination of LA-MRSA CC398 within the Danish pig production system, although other transmission routes may also have contributed. Consistent with this scenario, the genetic relatedness of isolates from different farms was positively correlated with the number of animal movements between the farms. American Society for Microbiology 2018-11-13 /pmc/articles/PMC6234867/ /pubmed/30425152 http://dx.doi.org/10.1128/mBio.02142-18 Text en Copyright © 2018 Sieber et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Sieber, Raphael N.
Skov, Robert L.
Nielsen, Jens
Schulz, Jana
Price, Lance B.
Aarestrup, Frank M.
Larsen, Anders R.
Stegger, Marc
Larsen, Jesper
Drivers and Dynamics of Methicillin-Resistant Livestock-Associated Staphylococcus aureus CC398 in Pigs and Humans in Denmark
title Drivers and Dynamics of Methicillin-Resistant Livestock-Associated Staphylococcus aureus CC398 in Pigs and Humans in Denmark
title_full Drivers and Dynamics of Methicillin-Resistant Livestock-Associated Staphylococcus aureus CC398 in Pigs and Humans in Denmark
title_fullStr Drivers and Dynamics of Methicillin-Resistant Livestock-Associated Staphylococcus aureus CC398 in Pigs and Humans in Denmark
title_full_unstemmed Drivers and Dynamics of Methicillin-Resistant Livestock-Associated Staphylococcus aureus CC398 in Pigs and Humans in Denmark
title_short Drivers and Dynamics of Methicillin-Resistant Livestock-Associated Staphylococcus aureus CC398 in Pigs and Humans in Denmark
title_sort drivers and dynamics of methicillin-resistant livestock-associated staphylococcus aureus cc398 in pigs and humans in denmark
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6234867/
https://www.ncbi.nlm.nih.gov/pubmed/30425152
http://dx.doi.org/10.1128/mBio.02142-18
work_keys_str_mv AT sieberraphaeln driversanddynamicsofmethicillinresistantlivestockassociatedstaphylococcusaureuscc398inpigsandhumansindenmark
AT skovrobertl driversanddynamicsofmethicillinresistantlivestockassociatedstaphylococcusaureuscc398inpigsandhumansindenmark
AT nielsenjens driversanddynamicsofmethicillinresistantlivestockassociatedstaphylococcusaureuscc398inpigsandhumansindenmark
AT schulzjana driversanddynamicsofmethicillinresistantlivestockassociatedstaphylococcusaureuscc398inpigsandhumansindenmark
AT pricelanceb driversanddynamicsofmethicillinresistantlivestockassociatedstaphylococcusaureuscc398inpigsandhumansindenmark
AT aarestrupfrankm driversanddynamicsofmethicillinresistantlivestockassociatedstaphylococcusaureuscc398inpigsandhumansindenmark
AT larsenandersr driversanddynamicsofmethicillinresistantlivestockassociatedstaphylococcusaureuscc398inpigsandhumansindenmark
AT steggermarc driversanddynamicsofmethicillinresistantlivestockassociatedstaphylococcusaureuscc398inpigsandhumansindenmark
AT larsenjesper driversanddynamicsofmethicillinresistantlivestockassociatedstaphylococcusaureuscc398inpigsandhumansindenmark