Cargando…

MicroRNA-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting Bcl2L10

BACKGROUND: Hepatocellular carcinoma (HCC) is known to feature several microRNA dysregulations. This study aimed to determine and investigate the prognostic value of microRNA (miRNA/miR)-18a and its role in regulating the progression of HCC. METHODS: miR-18a expressions in human HCC tissues, pair-ma...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xiaodong, Lu, Jian, Cao, Jisen, Ma, Bozhao, Gao, Chao, Qi, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6235330/
https://www.ncbi.nlm.nih.gov/pubmed/30519035
http://dx.doi.org/10.2147/OTT.S180971
_version_ 1783370858447765504
author Wang, Xiaodong
Lu, Jian
Cao, Jisen
Ma, Bozhao
Gao, Chao
Qi, Feng
author_facet Wang, Xiaodong
Lu, Jian
Cao, Jisen
Ma, Bozhao
Gao, Chao
Qi, Feng
author_sort Wang, Xiaodong
collection PubMed
description BACKGROUND: Hepatocellular carcinoma (HCC) is known to feature several microRNA dysregulations. This study aimed to determine and investigate the prognostic value of microRNA (miRNA/miR)-18a and its role in regulating the progression of HCC. METHODS: miR-18a expressions in human HCC tissues, pair-matched adjacent normal liver tissues as well as in HCC cell lines were determined by quantitative real-time PCR. The prognostic value of miR-18a was determined using Kaplan–Meier survival analysis and multivariable Cox regression assay. The ability of miR-18a in promoting HCC progression was verified in vitro. RESULTS: miR-18a expressions in HCC tissues and cells were more than twice those of the normal control group (P<0.05). miR-18a expression was associated with the alpha-fetoprotein (AFP) level, TNM stage, tumor size, and intrahepatic vascular invasion (P<0.05). Kaplan– Meier survival analysis revealed that HCC patients with high expression of miR-18a possessed a more unfavorable prognosis (log-rank P<0.001). Overexpression of miR-18a promoted cell apoptosis and proliferation, induced S phase transition, as well as enhanced the migration and invasion ability of HCC cells. miR-18a was found to directly target the downstream molecule Bcl2L10. Furthermore, overexpressing Bcl2L10 was able to partly reverse the promoting effects of miR-18a on HCC cell progression. CONCLUSION: miR-18a may serve as a prognostic biomarker of HCC as it is demonstrated to carry out a decisive role in HCC progression by promoting HCC cell invasion, migration, and proliferation through targeting Bcl2L10.
format Online
Article
Text
id pubmed-6235330
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-62353302018-12-05 MicroRNA-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting Bcl2L10 Wang, Xiaodong Lu, Jian Cao, Jisen Ma, Bozhao Gao, Chao Qi, Feng Onco Targets Ther Original Research BACKGROUND: Hepatocellular carcinoma (HCC) is known to feature several microRNA dysregulations. This study aimed to determine and investigate the prognostic value of microRNA (miRNA/miR)-18a and its role in regulating the progression of HCC. METHODS: miR-18a expressions in human HCC tissues, pair-matched adjacent normal liver tissues as well as in HCC cell lines were determined by quantitative real-time PCR. The prognostic value of miR-18a was determined using Kaplan–Meier survival analysis and multivariable Cox regression assay. The ability of miR-18a in promoting HCC progression was verified in vitro. RESULTS: miR-18a expressions in HCC tissues and cells were more than twice those of the normal control group (P<0.05). miR-18a expression was associated with the alpha-fetoprotein (AFP) level, TNM stage, tumor size, and intrahepatic vascular invasion (P<0.05). Kaplan– Meier survival analysis revealed that HCC patients with high expression of miR-18a possessed a more unfavorable prognosis (log-rank P<0.001). Overexpression of miR-18a promoted cell apoptosis and proliferation, induced S phase transition, as well as enhanced the migration and invasion ability of HCC cells. miR-18a was found to directly target the downstream molecule Bcl2L10. Furthermore, overexpressing Bcl2L10 was able to partly reverse the promoting effects of miR-18a on HCC cell progression. CONCLUSION: miR-18a may serve as a prognostic biomarker of HCC as it is demonstrated to carry out a decisive role in HCC progression by promoting HCC cell invasion, migration, and proliferation through targeting Bcl2L10. Dove Medical Press 2018-11-09 /pmc/articles/PMC6235330/ /pubmed/30519035 http://dx.doi.org/10.2147/OTT.S180971 Text en © 2018 Wang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Wang, Xiaodong
Lu, Jian
Cao, Jisen
Ma, Bozhao
Gao, Chao
Qi, Feng
MicroRNA-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting Bcl2L10
title MicroRNA-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting Bcl2L10
title_full MicroRNA-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting Bcl2L10
title_fullStr MicroRNA-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting Bcl2L10
title_full_unstemmed MicroRNA-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting Bcl2L10
title_short MicroRNA-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting Bcl2L10
title_sort microrna-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting bcl2l10
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6235330/
https://www.ncbi.nlm.nih.gov/pubmed/30519035
http://dx.doi.org/10.2147/OTT.S180971
work_keys_str_mv AT wangxiaodong microrna18apromoteshepatocellularcarcinomaproliferationmigrationandinvasionbytargetingbcl2l10
AT lujian microrna18apromoteshepatocellularcarcinomaproliferationmigrationandinvasionbytargetingbcl2l10
AT caojisen microrna18apromoteshepatocellularcarcinomaproliferationmigrationandinvasionbytargetingbcl2l10
AT mabozhao microrna18apromoteshepatocellularcarcinomaproliferationmigrationandinvasionbytargetingbcl2l10
AT gaochao microrna18apromoteshepatocellularcarcinomaproliferationmigrationandinvasionbytargetingbcl2l10
AT qifeng microrna18apromoteshepatocellularcarcinomaproliferationmigrationandinvasionbytargetingbcl2l10