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Contrôle de qualité virologique du sang transfusé dans la ville de Bukavu, Sud Kivu, République Démocratique du Congo

INTRODUCTION: In Bukavu, transfused blood is selected using rapid diagnostic tests (RDTs). These tests are easily performed without specialized equipments. This study aims to evaluate the virological quality of transfused blood assessed using rapid diagnostic tests. METHODS: A blood sample was drawn...

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Autores principales: Kashosi, Théophile Mitima, Mutendela, John Kivukuto, Mwenebitu, David Lupande, Maotela, Jeff Kabinda, Mubagwa, Kanigula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The African Field Epidemiology Network 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6235500/
https://www.ncbi.nlm.nih.gov/pubmed/30455822
http://dx.doi.org/10.11604/pamj.2018.30.193.13457
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author Kashosi, Théophile Mitima
Mutendela, John Kivukuto
Mwenebitu, David Lupande
Maotela, Jeff Kabinda
Mubagwa, Kanigula
author_facet Kashosi, Théophile Mitima
Mutendela, John Kivukuto
Mwenebitu, David Lupande
Maotela, Jeff Kabinda
Mubagwa, Kanigula
author_sort Kashosi, Théophile Mitima
collection PubMed
description INTRODUCTION: In Bukavu, transfused blood is selected using rapid diagnostic tests (RDTs). These tests are easily performed without specialized equipments. This study aims to evaluate the virological quality of transfused blood assessed using rapid diagnostic tests. METHODS: A blood sample was drawn from a blood bag and collected in a 4ml dry tube in 5 Health Care Facilities over a month. Counter analysis was performed on each sample using rapid tests and ELISA. Intrinsic and extrinsic values were calculated. Cohen’s kappa coefficient was used to evaluate the reliability of RDT compared with ELISA. RESULTS: Three hundred and twelve samples were collected; 5 samples were positive for one or the other virologic marker while 307 samples were negative in all the tests. However Elisa showed, out of the 307 samples which were RDT test negative, 15 other positive samples including 3 samples positive for HIV, 3 for HCV and 9 for HBV. In addition, ELISA validated some RDT-positive samples and contradicted other results. Sensitivity and positive predictive value from rapid diagnostic tests were very low. The reliability of these tests was satisfactory, medium or low. CONCLUSION: Blood assessed using RDTs poses a non negligible risk of viral infections. This study highlights the need for more reliable and efficient tests in our Health Care Facilities.
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spelling pubmed-62355002018-11-19 Contrôle de qualité virologique du sang transfusé dans la ville de Bukavu, Sud Kivu, République Démocratique du Congo Kashosi, Théophile Mitima Mutendela, John Kivukuto Mwenebitu, David Lupande Maotela, Jeff Kabinda Mubagwa, Kanigula Pan Afr Med J Research INTRODUCTION: In Bukavu, transfused blood is selected using rapid diagnostic tests (RDTs). These tests are easily performed without specialized equipments. This study aims to evaluate the virological quality of transfused blood assessed using rapid diagnostic tests. METHODS: A blood sample was drawn from a blood bag and collected in a 4ml dry tube in 5 Health Care Facilities over a month. Counter analysis was performed on each sample using rapid tests and ELISA. Intrinsic and extrinsic values were calculated. Cohen’s kappa coefficient was used to evaluate the reliability of RDT compared with ELISA. RESULTS: Three hundred and twelve samples were collected; 5 samples were positive for one or the other virologic marker while 307 samples were negative in all the tests. However Elisa showed, out of the 307 samples which were RDT test negative, 15 other positive samples including 3 samples positive for HIV, 3 for HCV and 9 for HBV. In addition, ELISA validated some RDT-positive samples and contradicted other results. Sensitivity and positive predictive value from rapid diagnostic tests were very low. The reliability of these tests was satisfactory, medium or low. CONCLUSION: Blood assessed using RDTs poses a non negligible risk of viral infections. This study highlights the need for more reliable and efficient tests in our Health Care Facilities. The African Field Epidemiology Network 2018-07-04 /pmc/articles/PMC6235500/ /pubmed/30455822 http://dx.doi.org/10.11604/pamj.2018.30.193.13457 Text en © Théophile Mitima Kashosi et al. http://creativecommons.org/licenses/by/2.0/ The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kashosi, Théophile Mitima
Mutendela, John Kivukuto
Mwenebitu, David Lupande
Maotela, Jeff Kabinda
Mubagwa, Kanigula
Contrôle de qualité virologique du sang transfusé dans la ville de Bukavu, Sud Kivu, République Démocratique du Congo
title Contrôle de qualité virologique du sang transfusé dans la ville de Bukavu, Sud Kivu, République Démocratique du Congo
title_full Contrôle de qualité virologique du sang transfusé dans la ville de Bukavu, Sud Kivu, République Démocratique du Congo
title_fullStr Contrôle de qualité virologique du sang transfusé dans la ville de Bukavu, Sud Kivu, République Démocratique du Congo
title_full_unstemmed Contrôle de qualité virologique du sang transfusé dans la ville de Bukavu, Sud Kivu, République Démocratique du Congo
title_short Contrôle de qualité virologique du sang transfusé dans la ville de Bukavu, Sud Kivu, République Démocratique du Congo
title_sort contrôle de qualité virologique du sang transfusé dans la ville de bukavu, sud kivu, république démocratique du congo
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6235500/
https://www.ncbi.nlm.nih.gov/pubmed/30455822
http://dx.doi.org/10.11604/pamj.2018.30.193.13457
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