Cargando…

Mode of delivery by an ulcerative colitis mother in a case of twins: Immunological differences in cord blood and placenta

AIM: To understand the effects of delivery mode on the immune cells frequency and function in cord blood and placenta. METHODS: We evaluated immunological differences in cord blood and placental tissues for a case of twins one of which delivered vaginally while the other delivered by caesarian secti...

Descripción completa

Detalles Bibliográficos
Autores principales: Dunsmore, Garett, Koleva, Petya, Sutton, Reed Taylor, Ambrosio, Lindsy, Huang, Vivian, Elahi, Shokrollah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6235805/
https://www.ncbi.nlm.nih.gov/pubmed/30479465
http://dx.doi.org/10.3748/wjg.v24.i42.4787
_version_ 1783370920810774528
author Dunsmore, Garett
Koleva, Petya
Sutton, Reed Taylor
Ambrosio, Lindsy
Huang, Vivian
Elahi, Shokrollah
author_facet Dunsmore, Garett
Koleva, Petya
Sutton, Reed Taylor
Ambrosio, Lindsy
Huang, Vivian
Elahi, Shokrollah
author_sort Dunsmore, Garett
collection PubMed
description AIM: To understand the effects of delivery mode on the immune cells frequency and function in cord blood and placenta. METHODS: We evaluated immunological differences in cord blood and placental tissues for a case of twins one of which delivered vaginally while the other delivered by caesarian section (C-section). Cord blood mononuclear cells were isolated and placenta tissues were processed for cell isolation. Immune phenotyping was performed by flow cytometry methods following staining for T cells, natural killer (NK) cells, monocytes, neutrophils and CD71(+) erythroid cells in both cord blood and placenta tissues. In addition, fetal calprotectin of twins was measured 12 wk after birth. RESULTS: We found lower percentages of immune cells (e.g. T cells, monocytes and neutrophils) in the cord blood of C-section delivered compared to vaginally delivered newborn. In contrast, percentages of monocytes and neutrophils were > 2 folds higher in the placental tissues of C-section delivered newborn. More importantly, we observed lower percentages of CD71(+) erythroid cells in both cord blood and placental tissues of C-section delivered case. Lower CD71(+) erythroid cells were associated with a more pro-inflammatory milieu at the fetomaternal interface reflected by higher expression of inhibitory receptors on CD4(+) T cells, higher frequency of monocytes and neutrophils. Furthermore, type of delivery impacted the gene expression profile in CD71(+) erythroid cells. Finally, we found that C-section delivered child had > 20-fold higher FCP in his fecal sample at 12 wk of age. CONCLUSION: Mode of delivery impacted immune cells profile in cord blood/placenta. In particular frequency of immunosuppressive CD71(+) erythroid cells was reduced in C-section delivered newborn.
format Online
Article
Text
id pubmed-6235805
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Baishideng Publishing Group Inc
record_format MEDLINE/PubMed
spelling pubmed-62358052018-11-26 Mode of delivery by an ulcerative colitis mother in a case of twins: Immunological differences in cord blood and placenta Dunsmore, Garett Koleva, Petya Sutton, Reed Taylor Ambrosio, Lindsy Huang, Vivian Elahi, Shokrollah World J Gastroenterol Case Control Study AIM: To understand the effects of delivery mode on the immune cells frequency and function in cord blood and placenta. METHODS: We evaluated immunological differences in cord blood and placental tissues for a case of twins one of which delivered vaginally while the other delivered by caesarian section (C-section). Cord blood mononuclear cells were isolated and placenta tissues were processed for cell isolation. Immune phenotyping was performed by flow cytometry methods following staining for T cells, natural killer (NK) cells, monocytes, neutrophils and CD71(+) erythroid cells in both cord blood and placenta tissues. In addition, fetal calprotectin of twins was measured 12 wk after birth. RESULTS: We found lower percentages of immune cells (e.g. T cells, monocytes and neutrophils) in the cord blood of C-section delivered compared to vaginally delivered newborn. In contrast, percentages of monocytes and neutrophils were > 2 folds higher in the placental tissues of C-section delivered newborn. More importantly, we observed lower percentages of CD71(+) erythroid cells in both cord blood and placental tissues of C-section delivered case. Lower CD71(+) erythroid cells were associated with a more pro-inflammatory milieu at the fetomaternal interface reflected by higher expression of inhibitory receptors on CD4(+) T cells, higher frequency of monocytes and neutrophils. Furthermore, type of delivery impacted the gene expression profile in CD71(+) erythroid cells. Finally, we found that C-section delivered child had > 20-fold higher FCP in his fecal sample at 12 wk of age. CONCLUSION: Mode of delivery impacted immune cells profile in cord blood/placenta. In particular frequency of immunosuppressive CD71(+) erythroid cells was reduced in C-section delivered newborn. Baishideng Publishing Group Inc 2018-11-14 2018-11-14 /pmc/articles/PMC6235805/ /pubmed/30479465 http://dx.doi.org/10.3748/wjg.v24.i42.4787 Text en ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Case Control Study
Dunsmore, Garett
Koleva, Petya
Sutton, Reed Taylor
Ambrosio, Lindsy
Huang, Vivian
Elahi, Shokrollah
Mode of delivery by an ulcerative colitis mother in a case of twins: Immunological differences in cord blood and placenta
title Mode of delivery by an ulcerative colitis mother in a case of twins: Immunological differences in cord blood and placenta
title_full Mode of delivery by an ulcerative colitis mother in a case of twins: Immunological differences in cord blood and placenta
title_fullStr Mode of delivery by an ulcerative colitis mother in a case of twins: Immunological differences in cord blood and placenta
title_full_unstemmed Mode of delivery by an ulcerative colitis mother in a case of twins: Immunological differences in cord blood and placenta
title_short Mode of delivery by an ulcerative colitis mother in a case of twins: Immunological differences in cord blood and placenta
title_sort mode of delivery by an ulcerative colitis mother in a case of twins: immunological differences in cord blood and placenta
topic Case Control Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6235805/
https://www.ncbi.nlm.nih.gov/pubmed/30479465
http://dx.doi.org/10.3748/wjg.v24.i42.4787
work_keys_str_mv AT dunsmoregarett modeofdeliverybyanulcerativecolitismotherinacaseoftwinsimmunologicaldifferencesincordbloodandplacenta
AT kolevapetya modeofdeliverybyanulcerativecolitismotherinacaseoftwinsimmunologicaldifferencesincordbloodandplacenta
AT suttonreedtaylor modeofdeliverybyanulcerativecolitismotherinacaseoftwinsimmunologicaldifferencesincordbloodandplacenta
AT ambrosiolindsy modeofdeliverybyanulcerativecolitismotherinacaseoftwinsimmunologicaldifferencesincordbloodandplacenta
AT huangvivian modeofdeliverybyanulcerativecolitismotherinacaseoftwinsimmunologicaldifferencesincordbloodandplacenta
AT elahishokrollah modeofdeliverybyanulcerativecolitismotherinacaseoftwinsimmunologicaldifferencesincordbloodandplacenta