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Microbiomes and chemical components of feed water and membrane-attached biofilm in reverse osmosis system to treat membrane bioreactor effluents

Reverse osmosis (RO) system at a stage after membrane bioreactor (MBR) is used for the wastewater treatment and reclamation. One of the most serious problems in this system is membrane fouling caused by biofilm formation. Here, microbiomes and chemical components of the feed water and membrane-attac...

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Autores principales: Inaba, Tomohiro, Hori, Tomoyuki, Aizawa, Hidenobu, Sato, Yuya, Ogata, Atsushi, Habe, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6235981/
https://www.ncbi.nlm.nih.gov/pubmed/30429505
http://dx.doi.org/10.1038/s41598-018-35156-2
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author Inaba, Tomohiro
Hori, Tomoyuki
Aizawa, Hidenobu
Sato, Yuya
Ogata, Atsushi
Habe, Hiroshi
author_facet Inaba, Tomohiro
Hori, Tomoyuki
Aizawa, Hidenobu
Sato, Yuya
Ogata, Atsushi
Habe, Hiroshi
author_sort Inaba, Tomohiro
collection PubMed
description Reverse osmosis (RO) system at a stage after membrane bioreactor (MBR) is used for the wastewater treatment and reclamation. One of the most serious problems in this system is membrane fouling caused by biofilm formation. Here, microbiomes and chemical components of the feed water and membrane-attached biofilm of RO system to treat MBR effluents were investigated by non-destructive confocal reflection microscopy, excitation-emission fluorescence spectroscopy and high-throughput sequencing of 16S rRNA genes. The microscopic visualization indicated that the biofilm contained large amounts of microbial cells (0.5 ± 0.3~3.9 ± 2.3 µm(3)/µm(2)) and the extracellular polysaccharides (3.3 ± 1.7~9.4 ± 5.1 µm(3)/µm(2)) and proteins (1.0 ± 0.2~1.3 ± 0.1 µm(3)/µm(2)). The spectroscopic analysis identified the humic and/or fulvic acid-like substances and protein-like substances as the main membrane foulants. High-throughput sequencing showed that Pseudomonas spp. and other heterotrophic bacteria dominated the feed water microbiomes. Meanwhile, the biofilm microbiomes were composed of diverse bacteria, among which operational taxonomic units related to the autotrophic Hydrogenophaga pseudoflava and Blastochloris viridis were abundant, accounting for up to 22.9 ± 4.1% and 3.1 ± 0.4% of the total, respectively. These results demonstrated that the minor autotrophic bacteria in the feed water played pivotal roles in the formation of polysaccharide- and protein-rich biofilm on RO membrane, thereby causing membrane fouling of RO system.
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spelling pubmed-62359812018-11-20 Microbiomes and chemical components of feed water and membrane-attached biofilm in reverse osmosis system to treat membrane bioreactor effluents Inaba, Tomohiro Hori, Tomoyuki Aizawa, Hidenobu Sato, Yuya Ogata, Atsushi Habe, Hiroshi Sci Rep Article Reverse osmosis (RO) system at a stage after membrane bioreactor (MBR) is used for the wastewater treatment and reclamation. One of the most serious problems in this system is membrane fouling caused by biofilm formation. Here, microbiomes and chemical components of the feed water and membrane-attached biofilm of RO system to treat MBR effluents were investigated by non-destructive confocal reflection microscopy, excitation-emission fluorescence spectroscopy and high-throughput sequencing of 16S rRNA genes. The microscopic visualization indicated that the biofilm contained large amounts of microbial cells (0.5 ± 0.3~3.9 ± 2.3 µm(3)/µm(2)) and the extracellular polysaccharides (3.3 ± 1.7~9.4 ± 5.1 µm(3)/µm(2)) and proteins (1.0 ± 0.2~1.3 ± 0.1 µm(3)/µm(2)). The spectroscopic analysis identified the humic and/or fulvic acid-like substances and protein-like substances as the main membrane foulants. High-throughput sequencing showed that Pseudomonas spp. and other heterotrophic bacteria dominated the feed water microbiomes. Meanwhile, the biofilm microbiomes were composed of diverse bacteria, among which operational taxonomic units related to the autotrophic Hydrogenophaga pseudoflava and Blastochloris viridis were abundant, accounting for up to 22.9 ± 4.1% and 3.1 ± 0.4% of the total, respectively. These results demonstrated that the minor autotrophic bacteria in the feed water played pivotal roles in the formation of polysaccharide- and protein-rich biofilm on RO membrane, thereby causing membrane fouling of RO system. Nature Publishing Group UK 2018-11-14 /pmc/articles/PMC6235981/ /pubmed/30429505 http://dx.doi.org/10.1038/s41598-018-35156-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Inaba, Tomohiro
Hori, Tomoyuki
Aizawa, Hidenobu
Sato, Yuya
Ogata, Atsushi
Habe, Hiroshi
Microbiomes and chemical components of feed water and membrane-attached biofilm in reverse osmosis system to treat membrane bioreactor effluents
title Microbiomes and chemical components of feed water and membrane-attached biofilm in reverse osmosis system to treat membrane bioreactor effluents
title_full Microbiomes and chemical components of feed water and membrane-attached biofilm in reverse osmosis system to treat membrane bioreactor effluents
title_fullStr Microbiomes and chemical components of feed water and membrane-attached biofilm in reverse osmosis system to treat membrane bioreactor effluents
title_full_unstemmed Microbiomes and chemical components of feed water and membrane-attached biofilm in reverse osmosis system to treat membrane bioreactor effluents
title_short Microbiomes and chemical components of feed water and membrane-attached biofilm in reverse osmosis system to treat membrane bioreactor effluents
title_sort microbiomes and chemical components of feed water and membrane-attached biofilm in reverse osmosis system to treat membrane bioreactor effluents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6235981/
https://www.ncbi.nlm.nih.gov/pubmed/30429505
http://dx.doi.org/10.1038/s41598-018-35156-2
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