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Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord

Different sources of mesenchymal stem cells (MSCs) may differ in their biological characteristics, which are important for their clinical application. In the present study, MSCs were isolated from human exfoliated deciduous teeth (SHED), bone marrow, gingival tissue and umbilical cord tissue, and th...

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Autores principales: Li, Jing, Xu, Shi-Qing, Zhao, Yu-Ming, Yu, Shi, Ge, Li-Hong, Xu, Bao-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236220/
https://www.ncbi.nlm.nih.gov/pubmed/30272340
http://dx.doi.org/10.3892/mmr.2018.9501
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author Li, Jing
Xu, Shi-Qing
Zhao, Yu-Ming
Yu, Shi
Ge, Li-Hong
Xu, Bao-Hua
author_facet Li, Jing
Xu, Shi-Qing
Zhao, Yu-Ming
Yu, Shi
Ge, Li-Hong
Xu, Bao-Hua
author_sort Li, Jing
collection PubMed
description Different sources of mesenchymal stem cells (MSCs) may differ in their biological characteristics, which are important for their clinical application. In the present study, MSCs were isolated from human exfoliated deciduous teeth (SHED), bone marrow, gingival tissue and umbilical cord tissue, and their biological characteristics including surface markers, proliferation capacity, tumorigenicity and immunogenicity were analyzed by flow cytometric analysis, ELISA and co-culture with human lymphocytes, respectively. The results indicated that all four types of stem cells obtained from different sources expressed MSC surface markers, and they did not show tumorigenicity either in vivo or in vitro. Stem cells from SHED exhibited the strongest proliferation capacity. Umbilical cord-derived MSCs displayed the strongest immunomodulatory ability, while bone marrow MSCs exhibited the best antigen-presenting potential in response to interferon-γ stimulation. These results provide information on MSCs derived from different tissues, which may be helpful in their clinical application.
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spelling pubmed-62362202018-11-19 Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord Li, Jing Xu, Shi-Qing Zhao, Yu-Ming Yu, Shi Ge, Li-Hong Xu, Bao-Hua Mol Med Rep Articles Different sources of mesenchymal stem cells (MSCs) may differ in their biological characteristics, which are important for their clinical application. In the present study, MSCs were isolated from human exfoliated deciduous teeth (SHED), bone marrow, gingival tissue and umbilical cord tissue, and their biological characteristics including surface markers, proliferation capacity, tumorigenicity and immunogenicity were analyzed by flow cytometric analysis, ELISA and co-culture with human lymphocytes, respectively. The results indicated that all four types of stem cells obtained from different sources expressed MSC surface markers, and they did not show tumorigenicity either in vivo or in vitro. Stem cells from SHED exhibited the strongest proliferation capacity. Umbilical cord-derived MSCs displayed the strongest immunomodulatory ability, while bone marrow MSCs exhibited the best antigen-presenting potential in response to interferon-γ stimulation. These results provide information on MSCs derived from different tissues, which may be helpful in their clinical application. D.A. Spandidos 2018-12 2018-09-20 /pmc/articles/PMC6236220/ /pubmed/30272340 http://dx.doi.org/10.3892/mmr.2018.9501 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Jing
Xu, Shi-Qing
Zhao, Yu-Ming
Yu, Shi
Ge, Li-Hong
Xu, Bao-Hua
Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord
title Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord
title_full Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord
title_fullStr Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord
title_full_unstemmed Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord
title_short Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord
title_sort comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236220/
https://www.ncbi.nlm.nih.gov/pubmed/30272340
http://dx.doi.org/10.3892/mmr.2018.9501
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