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HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure

High-mountain sickness is characterized by brain and pulmonary edema and cognitive deficits. The definition can be fulfilled by a rat model of high-altitude exposure (HAE) used in the present study. This study aimed to investigate the protective effect of hyperbaric oxygen therapy (HBO(2)T) and to d...

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Autores principales: Wu, Hsing-Hsien, Niu, Ko-Chi, Lin, Cheng-Hsien, Lin, Hung-Jung, Chang, Ching-Ping, Wang, Chia-Ti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236768/
https://www.ncbi.nlm.nih.gov/pubmed/30515398
http://dx.doi.org/10.1155/2018/4608150
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author Wu, Hsing-Hsien
Niu, Ko-Chi
Lin, Cheng-Hsien
Lin, Hung-Jung
Chang, Ching-Ping
Wang, Chia-Ti
author_facet Wu, Hsing-Hsien
Niu, Ko-Chi
Lin, Cheng-Hsien
Lin, Hung-Jung
Chang, Ching-Ping
Wang, Chia-Ti
author_sort Wu, Hsing-Hsien
collection PubMed
description High-mountain sickness is characterized by brain and pulmonary edema and cognitive deficits. The definition can be fulfilled by a rat model of high-altitude exposure (HAE) used in the present study. This study aimed to investigate the protective effect of hyperbaric oxygen therapy (HBO(2)T) and to determine the underlying mechanisms. Rats were subjected to an HAE (9.7% O(2) at 0.47 absolute atmosphere of 6,000 m for 3 days). Immediately after termination of HAE, rats were treated with HBO(2)T (100% O(2) at 2.0 absolute atmosphere for 1 hour per day for 5 consecutive days) or non-HBO(2)T (21% O(2) at 1.0 absolute atmosphere for 1 hour per day for 5 consecutive days). As compared to non-HAE+non-HBO(2)T controls, the HAE+non-HBO(2)T rats exhibited brain edema and resulted in cognitive deficits, reduced food and water consumption, body weight loss, increased cerebral inflammation and oxidative stress, and pulmonary edema. HBO(2)T increased expression of both hippocampus and lung heat shock protein (HSP-70) and also reversed the HAE-induced brain and pulmonary edema, cognitive deficits, reduced food and water consumption, body weight loss, and brain inflammation and oxidative stress. Decreasing the overexpression of HSP-70 in both hippocampus and lung tissues with HSP-70 antibodies significantly attenuated the beneficial effects exerted by HBO(2)T in HAE rats. Our data provide in vivo evidence that HBO(2)T works on a remodeling of brain/lung to exert a protective effect against simulated high-mountain sickness via enhancing HSP-70 expression in HAE rats.
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spelling pubmed-62367682018-12-04 HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure Wu, Hsing-Hsien Niu, Ko-Chi Lin, Cheng-Hsien Lin, Hung-Jung Chang, Ching-Ping Wang, Chia-Ti Biomed Res Int Research Article High-mountain sickness is characterized by brain and pulmonary edema and cognitive deficits. The definition can be fulfilled by a rat model of high-altitude exposure (HAE) used in the present study. This study aimed to investigate the protective effect of hyperbaric oxygen therapy (HBO(2)T) and to determine the underlying mechanisms. Rats were subjected to an HAE (9.7% O(2) at 0.47 absolute atmosphere of 6,000 m for 3 days). Immediately after termination of HAE, rats were treated with HBO(2)T (100% O(2) at 2.0 absolute atmosphere for 1 hour per day for 5 consecutive days) or non-HBO(2)T (21% O(2) at 1.0 absolute atmosphere for 1 hour per day for 5 consecutive days). As compared to non-HAE+non-HBO(2)T controls, the HAE+non-HBO(2)T rats exhibited brain edema and resulted in cognitive deficits, reduced food and water consumption, body weight loss, increased cerebral inflammation and oxidative stress, and pulmonary edema. HBO(2)T increased expression of both hippocampus and lung heat shock protein (HSP-70) and also reversed the HAE-induced brain and pulmonary edema, cognitive deficits, reduced food and water consumption, body weight loss, and brain inflammation and oxidative stress. Decreasing the overexpression of HSP-70 in both hippocampus and lung tissues with HSP-70 antibodies significantly attenuated the beneficial effects exerted by HBO(2)T in HAE rats. Our data provide in vivo evidence that HBO(2)T works on a remodeling of brain/lung to exert a protective effect against simulated high-mountain sickness via enhancing HSP-70 expression in HAE rats. Hindawi 2018-11-01 /pmc/articles/PMC6236768/ /pubmed/30515398 http://dx.doi.org/10.1155/2018/4608150 Text en Copyright © 2018 Hsing-Hsien Wu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wu, Hsing-Hsien
Niu, Ko-Chi
Lin, Cheng-Hsien
Lin, Hung-Jung
Chang, Ching-Ping
Wang, Chia-Ti
HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure
title HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure
title_full HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure
title_fullStr HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure
title_full_unstemmed HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure
title_short HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure
title_sort hsp-70-mediated hyperbaric oxygen reduces brain and pulmonary edema and cognitive deficits in rats in a simulated high-altitude exposure
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236768/
https://www.ncbi.nlm.nih.gov/pubmed/30515398
http://dx.doi.org/10.1155/2018/4608150
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