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HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure
High-mountain sickness is characterized by brain and pulmonary edema and cognitive deficits. The definition can be fulfilled by a rat model of high-altitude exposure (HAE) used in the present study. This study aimed to investigate the protective effect of hyperbaric oxygen therapy (HBO(2)T) and to d...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236768/ https://www.ncbi.nlm.nih.gov/pubmed/30515398 http://dx.doi.org/10.1155/2018/4608150 |
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author | Wu, Hsing-Hsien Niu, Ko-Chi Lin, Cheng-Hsien Lin, Hung-Jung Chang, Ching-Ping Wang, Chia-Ti |
author_facet | Wu, Hsing-Hsien Niu, Ko-Chi Lin, Cheng-Hsien Lin, Hung-Jung Chang, Ching-Ping Wang, Chia-Ti |
author_sort | Wu, Hsing-Hsien |
collection | PubMed |
description | High-mountain sickness is characterized by brain and pulmonary edema and cognitive deficits. The definition can be fulfilled by a rat model of high-altitude exposure (HAE) used in the present study. This study aimed to investigate the protective effect of hyperbaric oxygen therapy (HBO(2)T) and to determine the underlying mechanisms. Rats were subjected to an HAE (9.7% O(2) at 0.47 absolute atmosphere of 6,000 m for 3 days). Immediately after termination of HAE, rats were treated with HBO(2)T (100% O(2) at 2.0 absolute atmosphere for 1 hour per day for 5 consecutive days) or non-HBO(2)T (21% O(2) at 1.0 absolute atmosphere for 1 hour per day for 5 consecutive days). As compared to non-HAE+non-HBO(2)T controls, the HAE+non-HBO(2)T rats exhibited brain edema and resulted in cognitive deficits, reduced food and water consumption, body weight loss, increased cerebral inflammation and oxidative stress, and pulmonary edema. HBO(2)T increased expression of both hippocampus and lung heat shock protein (HSP-70) and also reversed the HAE-induced brain and pulmonary edema, cognitive deficits, reduced food and water consumption, body weight loss, and brain inflammation and oxidative stress. Decreasing the overexpression of HSP-70 in both hippocampus and lung tissues with HSP-70 antibodies significantly attenuated the beneficial effects exerted by HBO(2)T in HAE rats. Our data provide in vivo evidence that HBO(2)T works on a remodeling of brain/lung to exert a protective effect against simulated high-mountain sickness via enhancing HSP-70 expression in HAE rats. |
format | Online Article Text |
id | pubmed-6236768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-62367682018-12-04 HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure Wu, Hsing-Hsien Niu, Ko-Chi Lin, Cheng-Hsien Lin, Hung-Jung Chang, Ching-Ping Wang, Chia-Ti Biomed Res Int Research Article High-mountain sickness is characterized by brain and pulmonary edema and cognitive deficits. The definition can be fulfilled by a rat model of high-altitude exposure (HAE) used in the present study. This study aimed to investigate the protective effect of hyperbaric oxygen therapy (HBO(2)T) and to determine the underlying mechanisms. Rats were subjected to an HAE (9.7% O(2) at 0.47 absolute atmosphere of 6,000 m for 3 days). Immediately after termination of HAE, rats were treated with HBO(2)T (100% O(2) at 2.0 absolute atmosphere for 1 hour per day for 5 consecutive days) or non-HBO(2)T (21% O(2) at 1.0 absolute atmosphere for 1 hour per day for 5 consecutive days). As compared to non-HAE+non-HBO(2)T controls, the HAE+non-HBO(2)T rats exhibited brain edema and resulted in cognitive deficits, reduced food and water consumption, body weight loss, increased cerebral inflammation and oxidative stress, and pulmonary edema. HBO(2)T increased expression of both hippocampus and lung heat shock protein (HSP-70) and also reversed the HAE-induced brain and pulmonary edema, cognitive deficits, reduced food and water consumption, body weight loss, and brain inflammation and oxidative stress. Decreasing the overexpression of HSP-70 in both hippocampus and lung tissues with HSP-70 antibodies significantly attenuated the beneficial effects exerted by HBO(2)T in HAE rats. Our data provide in vivo evidence that HBO(2)T works on a remodeling of brain/lung to exert a protective effect against simulated high-mountain sickness via enhancing HSP-70 expression in HAE rats. Hindawi 2018-11-01 /pmc/articles/PMC6236768/ /pubmed/30515398 http://dx.doi.org/10.1155/2018/4608150 Text en Copyright © 2018 Hsing-Hsien Wu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wu, Hsing-Hsien Niu, Ko-Chi Lin, Cheng-Hsien Lin, Hung-Jung Chang, Ching-Ping Wang, Chia-Ti HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure |
title | HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure |
title_full | HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure |
title_fullStr | HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure |
title_full_unstemmed | HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure |
title_short | HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure |
title_sort | hsp-70-mediated hyperbaric oxygen reduces brain and pulmonary edema and cognitive deficits in rats in a simulated high-altitude exposure |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236768/ https://www.ncbi.nlm.nih.gov/pubmed/30515398 http://dx.doi.org/10.1155/2018/4608150 |
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