Purification and Characterization of Glutathione Binding Protein GsiB from Escherichia coli

OBJECTIVES: To purify and characterize the glutathione binding protein GsiB of glutathione importer (GSI) in Escherichia coli (E. coli). RESULTS: The coding sequence of GsiB was cloned from E. coli MG1655 and expressed in BL21(DE3). GsiB protein was expressed and purified to homogeneity using Ni-aff...

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Detalles Bibliográficos
Autores principales: Wang, Zhongshan, Xia, Xiaokun, Zhang, Meixian, Fang, Jiawei, Li, Yanqiang, Zhang, Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236770/
https://www.ncbi.nlm.nih.gov/pubmed/30515393
http://dx.doi.org/10.1155/2018/3429569

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