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Multiplex microRNA imaging in living cells using DNA-capped-Au assembled hydrogels

Non-invasively imaging multiplex microRNAs (miRNAs) in living cells is pivotal to understanding their physiological functions and pathological development due to the key regulatory roles of miRNAs in gene expression. However, developing smart delivery systems with large gene loading capacity, biocom...

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Autores principales: Meng, Xiangdan, Zhang, Kai, Dai, Wenhao, Cao, Yu, Yang, Fan, Dong, Haifeng, Zhang, Xueji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237120/
https://www.ncbi.nlm.nih.gov/pubmed/30542546
http://dx.doi.org/10.1039/c8sc02858c
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author Meng, Xiangdan
Zhang, Kai
Dai, Wenhao
Cao, Yu
Yang, Fan
Dong, Haifeng
Zhang, Xueji
author_facet Meng, Xiangdan
Zhang, Kai
Dai, Wenhao
Cao, Yu
Yang, Fan
Dong, Haifeng
Zhang, Xueji
author_sort Meng, Xiangdan
collection PubMed
description Non-invasively imaging multiplex microRNAs (miRNAs) in living cells is pivotal to understanding their physiological functions and pathological development due to the key regulatory roles of miRNAs in gene expression. However, developing smart delivery systems with large gene loading capacity, biocompatibility and responsiveness remains a significant challenge. Herein, we successfully incorporated DNA-capped Au nanoparticles (NPs) and their complementary fluorescent DNA sequences into a porous 3D hydrogel network (AuDH), in which hairpin-locked DNAzyme strands and active metal ions were loaded (AuDH/M(n+)/H) for simultaneously imaging multiplex miRNAs in living cells. After transfection into cells, the specific miRNAs trigger the strand-displacement reaction and sequentially activate the DNAzyme-assisted target recycling, leading to a strong increase in the corresponding fluorescence intensity for imaging. This enables simultaneous assessment of the abundance of multiplex cancer-related miRNAs, even if at a very low expression level, in different cells through the different fluorescence intensities due to the dual signal amplification, and the change in abundance of miRNAs induced by siRNA or miRNA mimics in living cells can also be efficiently monitored. The versatile and responsive DNA hydrogel system holds great potential for miRNA biomedical applications.
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spelling pubmed-62371202018-12-12 Multiplex microRNA imaging in living cells using DNA-capped-Au assembled hydrogels Meng, Xiangdan Zhang, Kai Dai, Wenhao Cao, Yu Yang, Fan Dong, Haifeng Zhang, Xueji Chem Sci Chemistry Non-invasively imaging multiplex microRNAs (miRNAs) in living cells is pivotal to understanding their physiological functions and pathological development due to the key regulatory roles of miRNAs in gene expression. However, developing smart delivery systems with large gene loading capacity, biocompatibility and responsiveness remains a significant challenge. Herein, we successfully incorporated DNA-capped Au nanoparticles (NPs) and their complementary fluorescent DNA sequences into a porous 3D hydrogel network (AuDH), in which hairpin-locked DNAzyme strands and active metal ions were loaded (AuDH/M(n+)/H) for simultaneously imaging multiplex miRNAs in living cells. After transfection into cells, the specific miRNAs trigger the strand-displacement reaction and sequentially activate the DNAzyme-assisted target recycling, leading to a strong increase in the corresponding fluorescence intensity for imaging. This enables simultaneous assessment of the abundance of multiplex cancer-related miRNAs, even if at a very low expression level, in different cells through the different fluorescence intensities due to the dual signal amplification, and the change in abundance of miRNAs induced by siRNA or miRNA mimics in living cells can also be efficiently monitored. The versatile and responsive DNA hydrogel system holds great potential for miRNA biomedical applications. Royal Society of Chemistry 2018-08-07 /pmc/articles/PMC6237120/ /pubmed/30542546 http://dx.doi.org/10.1039/c8sc02858c Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0)
spellingShingle Chemistry
Meng, Xiangdan
Zhang, Kai
Dai, Wenhao
Cao, Yu
Yang, Fan
Dong, Haifeng
Zhang, Xueji
Multiplex microRNA imaging in living cells using DNA-capped-Au assembled hydrogels
title Multiplex microRNA imaging in living cells using DNA-capped-Au assembled hydrogels
title_full Multiplex microRNA imaging in living cells using DNA-capped-Au assembled hydrogels
title_fullStr Multiplex microRNA imaging in living cells using DNA-capped-Au assembled hydrogels
title_full_unstemmed Multiplex microRNA imaging in living cells using DNA-capped-Au assembled hydrogels
title_short Multiplex microRNA imaging in living cells using DNA-capped-Au assembled hydrogels
title_sort multiplex microrna imaging in living cells using dna-capped-au assembled hydrogels
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237120/
https://www.ncbi.nlm.nih.gov/pubmed/30542546
http://dx.doi.org/10.1039/c8sc02858c
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