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The inflammasome in leprosy skin lesions: an immunohistochemical evaluation

OBJECTIVE: Leprosy is a chronic infectious disease presenting with a spectrum of clinical manifestations that correspond to the type of immune response that develops in the host. Factors that may be involved in this process include inflammasomes, cytosolic proteins responsible for the activation of...

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Autores principales: Silva, Luciana Mota, de Sousa, Jorge Rodrigues, Hirai, Kelly Emi, Dias, Leônidas Braga, Furlaneto, Ismari Perini, Carneiro, Francisca Regina Oliveira, de Souza Aarão, Tinara Leila, Sotto, Mirian Nacagami, Quaresma, Juarez Antonio Simões
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237140/
https://www.ncbi.nlm.nih.gov/pubmed/30519061
http://dx.doi.org/10.2147/IDR.S172806
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author Silva, Luciana Mota
de Sousa, Jorge Rodrigues
Hirai, Kelly Emi
Dias, Leônidas Braga
Furlaneto, Ismari Perini
Carneiro, Francisca Regina Oliveira
de Souza Aarão, Tinara Leila
Sotto, Mirian Nacagami
Quaresma, Juarez Antonio Simões
author_facet Silva, Luciana Mota
de Sousa, Jorge Rodrigues
Hirai, Kelly Emi
Dias, Leônidas Braga
Furlaneto, Ismari Perini
Carneiro, Francisca Regina Oliveira
de Souza Aarão, Tinara Leila
Sotto, Mirian Nacagami
Quaresma, Juarez Antonio Simões
author_sort Silva, Luciana Mota
collection PubMed
description OBJECTIVE: Leprosy is a chronic infectious disease presenting with a spectrum of clinical manifestations that correspond to the type of immune response that develops in the host. Factors that may be involved in this process include inflammasomes, cytosolic proteins responsible for the activation of caspase 1, IL-1β and IL-18 secretion, and induction of a type of death called pyroptosis. PATIENTS AND METHODS: We evaluated the expression of inflammasome markers (nucleotide-binding oligomerization domain-like receptor containing pyrin domain 1 [NLRP1], nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 [NLRP3], caspase 1, IL-1β, and IL-18) by immunohistochemistry in 43 samples of skin lesions of leprosy patients from the groups indeterminate (I) leprosy (13 patients), tuberculoid (TT) leprosy (15 patients), and lepromatous leprosy (LL; 15 patients). RESULTS: The evaluated markers were most upregulated in LL lesions, followed by lesions of TT leprosy and I leprosy. Differences were statistically significant between the I leprosy and LL leprosy forms and between the I leprosy and TT leprosy forms. Positive and significant correlations were found between IL-18 and caspase 1 in LL (r=0.7516, P=0.0012) and TT leprosy (r=0.7366, P=0.0017). In I leprosy, correlations were detected between caspase 1 and IL-1β (r=0.6412, P=0.0182), NLRP1 and IL-18 (r=0.5585, P=0.473), NLRP3 and IL-18 (r=0.6873, P=0.0094), and NLRP1 and NLRP3 (r=0.8040, P=0.0009). CONCLUSION: The expression of inflammasome markers in LL lesions indicates the ineffectiveness of this protein complex in controlling the infection. Caspase 1 may be involved in the pyroptotic cell death in the lepromatous form of the disease. Inflammasomes may act together in the initial phase of I leprosy; this phenomenon may influence the clinical outcome of the disease.
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spelling pubmed-62371402018-12-05 The inflammasome in leprosy skin lesions: an immunohistochemical evaluation Silva, Luciana Mota de Sousa, Jorge Rodrigues Hirai, Kelly Emi Dias, Leônidas Braga Furlaneto, Ismari Perini Carneiro, Francisca Regina Oliveira de Souza Aarão, Tinara Leila Sotto, Mirian Nacagami Quaresma, Juarez Antonio Simões Infect Drug Resist Original Research OBJECTIVE: Leprosy is a chronic infectious disease presenting with a spectrum of clinical manifestations that correspond to the type of immune response that develops in the host. Factors that may be involved in this process include inflammasomes, cytosolic proteins responsible for the activation of caspase 1, IL-1β and IL-18 secretion, and induction of a type of death called pyroptosis. PATIENTS AND METHODS: We evaluated the expression of inflammasome markers (nucleotide-binding oligomerization domain-like receptor containing pyrin domain 1 [NLRP1], nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 [NLRP3], caspase 1, IL-1β, and IL-18) by immunohistochemistry in 43 samples of skin lesions of leprosy patients from the groups indeterminate (I) leprosy (13 patients), tuberculoid (TT) leprosy (15 patients), and lepromatous leprosy (LL; 15 patients). RESULTS: The evaluated markers were most upregulated in LL lesions, followed by lesions of TT leprosy and I leprosy. Differences were statistically significant between the I leprosy and LL leprosy forms and between the I leprosy and TT leprosy forms. Positive and significant correlations were found between IL-18 and caspase 1 in LL (r=0.7516, P=0.0012) and TT leprosy (r=0.7366, P=0.0017). In I leprosy, correlations were detected between caspase 1 and IL-1β (r=0.6412, P=0.0182), NLRP1 and IL-18 (r=0.5585, P=0.473), NLRP3 and IL-18 (r=0.6873, P=0.0094), and NLRP1 and NLRP3 (r=0.8040, P=0.0009). CONCLUSION: The expression of inflammasome markers in LL lesions indicates the ineffectiveness of this protein complex in controlling the infection. Caspase 1 may be involved in the pyroptotic cell death in the lepromatous form of the disease. Inflammasomes may act together in the initial phase of I leprosy; this phenomenon may influence the clinical outcome of the disease. SAGE Publications 2018-11-12 /pmc/articles/PMC6237140/ /pubmed/30519061 http://dx.doi.org/10.2147/IDR.S172806 Text en © 2018 Silva et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Silva, Luciana Mota
de Sousa, Jorge Rodrigues
Hirai, Kelly Emi
Dias, Leônidas Braga
Furlaneto, Ismari Perini
Carneiro, Francisca Regina Oliveira
de Souza Aarão, Tinara Leila
Sotto, Mirian Nacagami
Quaresma, Juarez Antonio Simões
The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title_full The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title_fullStr The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title_full_unstemmed The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title_short The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title_sort inflammasome in leprosy skin lesions: an immunohistochemical evaluation
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237140/
https://www.ncbi.nlm.nih.gov/pubmed/30519061
http://dx.doi.org/10.2147/IDR.S172806
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