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Dynamic Analyses of the Short‐Term Effects of Different Bisphosphonates Using Intravital Two‐Photon Microscopy

Bisphosphonates are commonly used for the treatment of bone disorders such as osteoporosis; however, the mechanism by which they affect the dynamics of living mature osteoclasts in vivo remains unknown. Here, we describe the short‐term effects of different bisphosphonates on controlling the bone res...

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Autores principales: Kikuta, Junichi, Shirazaki, Mai, Sudo, Takao, Mizuno, Hiroki, Morimoto, Akito, Suehara, Riko, Minoshima, Masafumi, Kikuchi, Kazuya, Ishii, Masaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237210/
https://www.ncbi.nlm.nih.gov/pubmed/30460339
http://dx.doi.org/10.1002/jbm4.10057
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author Kikuta, Junichi
Shirazaki, Mai
Sudo, Takao
Mizuno, Hiroki
Morimoto, Akito
Suehara, Riko
Minoshima, Masafumi
Kikuchi, Kazuya
Ishii, Masaru
author_facet Kikuta, Junichi
Shirazaki, Mai
Sudo, Takao
Mizuno, Hiroki
Morimoto, Akito
Suehara, Riko
Minoshima, Masafumi
Kikuchi, Kazuya
Ishii, Masaru
author_sort Kikuta, Junichi
collection PubMed
description Bisphosphonates are commonly used for the treatment of bone disorders such as osteoporosis; however, the mechanism by which they affect the dynamics of living mature osteoclasts in vivo remains unknown. Here, we describe the short‐term effects of different bisphosphonates on controlling the bone resorptive activity of mature osteoclasts in living bone tissues of mice using intravital two‐photon microscopy with a pH‐sensing chemical fluorescent probe. Three types of nitrogen‐containing bisphosphonates, risedronate, alendronate, and minodronate, inhibited osteoclastic acidification during osteoporotic conditions just 12 hours after i.v. injection. Among the three types of drugs, risedronate was the most effective at increasing osteoclast motility and changing the localization of proton pumps, which led to an inhibition of bone resorption. Together, these results demonstrate that the intravital imaging system is a useful tool for evaluating the similarities and differences in currently used antibone resorptive drugs. © 2018 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.
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spelling pubmed-62372102018-11-20 Dynamic Analyses of the Short‐Term Effects of Different Bisphosphonates Using Intravital Two‐Photon Microscopy Kikuta, Junichi Shirazaki, Mai Sudo, Takao Mizuno, Hiroki Morimoto, Akito Suehara, Riko Minoshima, Masafumi Kikuchi, Kazuya Ishii, Masaru JBMR Plus Short Report Bisphosphonates are commonly used for the treatment of bone disorders such as osteoporosis; however, the mechanism by which they affect the dynamics of living mature osteoclasts in vivo remains unknown. Here, we describe the short‐term effects of different bisphosphonates on controlling the bone resorptive activity of mature osteoclasts in living bone tissues of mice using intravital two‐photon microscopy with a pH‐sensing chemical fluorescent probe. Three types of nitrogen‐containing bisphosphonates, risedronate, alendronate, and minodronate, inhibited osteoclastic acidification during osteoporotic conditions just 12 hours after i.v. injection. Among the three types of drugs, risedronate was the most effective at increasing osteoclast motility and changing the localization of proton pumps, which led to an inhibition of bone resorption. Together, these results demonstrate that the intravital imaging system is a useful tool for evaluating the similarities and differences in currently used antibone resorptive drugs. © 2018 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research. John Wiley and Sons Inc. 2018-06-22 /pmc/articles/PMC6237210/ /pubmed/30460339 http://dx.doi.org/10.1002/jbm4.10057 Text en © 2018 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Kikuta, Junichi
Shirazaki, Mai
Sudo, Takao
Mizuno, Hiroki
Morimoto, Akito
Suehara, Riko
Minoshima, Masafumi
Kikuchi, Kazuya
Ishii, Masaru
Dynamic Analyses of the Short‐Term Effects of Different Bisphosphonates Using Intravital Two‐Photon Microscopy
title Dynamic Analyses of the Short‐Term Effects of Different Bisphosphonates Using Intravital Two‐Photon Microscopy
title_full Dynamic Analyses of the Short‐Term Effects of Different Bisphosphonates Using Intravital Two‐Photon Microscopy
title_fullStr Dynamic Analyses of the Short‐Term Effects of Different Bisphosphonates Using Intravital Two‐Photon Microscopy
title_full_unstemmed Dynamic Analyses of the Short‐Term Effects of Different Bisphosphonates Using Intravital Two‐Photon Microscopy
title_short Dynamic Analyses of the Short‐Term Effects of Different Bisphosphonates Using Intravital Two‐Photon Microscopy
title_sort dynamic analyses of the short‐term effects of different bisphosphonates using intravital two‐photon microscopy
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237210/
https://www.ncbi.nlm.nih.gov/pubmed/30460339
http://dx.doi.org/10.1002/jbm4.10057
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