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Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer
Modulation or prevention of protein changes during the cholangiocarcinoma (CCA) process induced by Opisthorchis viverrini (Ov) infection may become a key strategy for prevention and treatment of CCA. Monitoring of such changes could lead to discovery of protein targets for CCA treatment. Curcumin ex...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237386/ https://www.ncbi.nlm.nih.gov/pubmed/30440021 http://dx.doi.org/10.1371/journal.pone.0207405 |
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author | Khoontawad, Jarinya Intuyod, Kitti Rucksaken, Rucksak Hongsrichan, Nuttanan Pairojkul, Chawalit Pinlaor, Porntip Boonmars, Thidarut Wongkham, Chaisiri Jones, Alun Plieskatt, Jordan Potriquet, Jeremy Bethony, Jeffrey M. Mulvenna, Jason Pinlaor, Somchai |
author_facet | Khoontawad, Jarinya Intuyod, Kitti Rucksaken, Rucksak Hongsrichan, Nuttanan Pairojkul, Chawalit Pinlaor, Porntip Boonmars, Thidarut Wongkham, Chaisiri Jones, Alun Plieskatt, Jordan Potriquet, Jeremy Bethony, Jeffrey M. Mulvenna, Jason Pinlaor, Somchai |
author_sort | Khoontawad, Jarinya |
collection | PubMed |
description | Modulation or prevention of protein changes during the cholangiocarcinoma (CCA) process induced by Opisthorchis viverrini (Ov) infection may become a key strategy for prevention and treatment of CCA. Monitoring of such changes could lead to discovery of protein targets for CCA treatment. Curcumin exerts anti-inflammatory and anti-CCA activities partly through its protein-modulatory ability. To support the potential use of curcumin and to discover novel target molecules for CCA treatment, we used a quantitative proteomic approach to investigate the effects of curcumin on protein changes in an Ov-induced CCA-harboring hamster model. Isobaric labelling and tandem mass spectrometry were used to compare the protein expression profiles of liver tissues from CCA hamsters with or without curcumin dietary supplementation. Among the dysregulated proteins, five were upregulated in liver tissues of CCA hamsters but markedly downregulated in the CCA hamsters supplemented with curcumin: S100A6, lumican, plastin-2, 14-3-3 zeta/delta and vimentin. Western blot and immunohistochemical analyses also showed similar expression patterns of these proteins in liver tissues of hamsters in the CCA and CCA + curcumin groups. Proteins such as clusterin and S100A10, involved in the NF-κB signaling pathway, an important signaling cascade involved in CCA genesis, were also upregulated in CCA hamsters and were then suppressed by curcumin treatment. Taken together, our results demonstrate the important changes in the proteome during the genesis of O. viverrini-induced CCA and provide an insight into the possible protein targets for prevention and treatment of this cancer. |
format | Online Article Text |
id | pubmed-6237386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62373862018-12-01 Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer Khoontawad, Jarinya Intuyod, Kitti Rucksaken, Rucksak Hongsrichan, Nuttanan Pairojkul, Chawalit Pinlaor, Porntip Boonmars, Thidarut Wongkham, Chaisiri Jones, Alun Plieskatt, Jordan Potriquet, Jeremy Bethony, Jeffrey M. Mulvenna, Jason Pinlaor, Somchai PLoS One Research Article Modulation or prevention of protein changes during the cholangiocarcinoma (CCA) process induced by Opisthorchis viverrini (Ov) infection may become a key strategy for prevention and treatment of CCA. Monitoring of such changes could lead to discovery of protein targets for CCA treatment. Curcumin exerts anti-inflammatory and anti-CCA activities partly through its protein-modulatory ability. To support the potential use of curcumin and to discover novel target molecules for CCA treatment, we used a quantitative proteomic approach to investigate the effects of curcumin on protein changes in an Ov-induced CCA-harboring hamster model. Isobaric labelling and tandem mass spectrometry were used to compare the protein expression profiles of liver tissues from CCA hamsters with or without curcumin dietary supplementation. Among the dysregulated proteins, five were upregulated in liver tissues of CCA hamsters but markedly downregulated in the CCA hamsters supplemented with curcumin: S100A6, lumican, plastin-2, 14-3-3 zeta/delta and vimentin. Western blot and immunohistochemical analyses also showed similar expression patterns of these proteins in liver tissues of hamsters in the CCA and CCA + curcumin groups. Proteins such as clusterin and S100A10, involved in the NF-κB signaling pathway, an important signaling cascade involved in CCA genesis, were also upregulated in CCA hamsters and were then suppressed by curcumin treatment. Taken together, our results demonstrate the important changes in the proteome during the genesis of O. viverrini-induced CCA and provide an insight into the possible protein targets for prevention and treatment of this cancer. Public Library of Science 2018-11-15 /pmc/articles/PMC6237386/ /pubmed/30440021 http://dx.doi.org/10.1371/journal.pone.0207405 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Khoontawad, Jarinya Intuyod, Kitti Rucksaken, Rucksak Hongsrichan, Nuttanan Pairojkul, Chawalit Pinlaor, Porntip Boonmars, Thidarut Wongkham, Chaisiri Jones, Alun Plieskatt, Jordan Potriquet, Jeremy Bethony, Jeffrey M. Mulvenna, Jason Pinlaor, Somchai Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer |
title | Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer |
title_full | Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer |
title_fullStr | Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer |
title_full_unstemmed | Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer |
title_short | Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer |
title_sort | discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237386/ https://www.ncbi.nlm.nih.gov/pubmed/30440021 http://dx.doi.org/10.1371/journal.pone.0207405 |
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