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Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential
Hepatocellular carcinoma (HCC) is one of the most common causes for cancer‐related death worldwide with rapidly increasing incidence and mortality rates. As for other types of cancers, also in HCC cancer stem cells (CSCs) are thought to be responsible for tumour initiation, progression and therapy f...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237557/ https://www.ncbi.nlm.nih.gov/pubmed/30280520 http://dx.doi.org/10.1111/jcmm.13911 |
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author | Muenzner, Julienne K. Kunze, Philipp Lindner, Pablo Polaschek, Sandra Menke, Kira Eckstein, Markus Geppert, Carol I. Chanvorachote, Pithi Baeuerle, Tobias Hartmann, Arndt Schneider‐Stock, Regine |
author_facet | Muenzner, Julienne K. Kunze, Philipp Lindner, Pablo Polaschek, Sandra Menke, Kira Eckstein, Markus Geppert, Carol I. Chanvorachote, Pithi Baeuerle, Tobias Hartmann, Arndt Schneider‐Stock, Regine |
author_sort | Muenzner, Julienne K. |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is one of the most common causes for cancer‐related death worldwide with rapidly increasing incidence and mortality rates. As for other types of cancers, also in HCC cancer stem cells (CSCs) are thought to be responsible for tumour initiation, progression and therapy failure. However, as rare subpopulations of tumour tissue, CSCs are difficult to isolate, thus making the development of suitable and reliable model systems necessary. In our study, we generated HepG2 subclones with enriched CSC potential by application of the spheroid formation method and subsequent single‐cell cloning. Analyses in several 2D and 3D cell culture systems as well as a panel of functional assays both in vitro and in vivo revealed that the generated subclones displayed characteristic and sustained features of tumour initiating cells as well as highly aggressive properties related to tumour progression and metastasis. These characteristics could clearly be correlated with the expression of CSC markers that might have prognostic value in the clinical HCC setting. Therefore, we conclude that our CSC enriched HepG2 clones certainly represent suitable model systems to study the role of CSCs during HCC initiation, progression and drug resistance. |
format | Online Article Text |
id | pubmed-6237557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62375572018-12-01 Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential Muenzner, Julienne K. Kunze, Philipp Lindner, Pablo Polaschek, Sandra Menke, Kira Eckstein, Markus Geppert, Carol I. Chanvorachote, Pithi Baeuerle, Tobias Hartmann, Arndt Schneider‐Stock, Regine J Cell Mol Med Original Articles Hepatocellular carcinoma (HCC) is one of the most common causes for cancer‐related death worldwide with rapidly increasing incidence and mortality rates. As for other types of cancers, also in HCC cancer stem cells (CSCs) are thought to be responsible for tumour initiation, progression and therapy failure. However, as rare subpopulations of tumour tissue, CSCs are difficult to isolate, thus making the development of suitable and reliable model systems necessary. In our study, we generated HepG2 subclones with enriched CSC potential by application of the spheroid formation method and subsequent single‐cell cloning. Analyses in several 2D and 3D cell culture systems as well as a panel of functional assays both in vitro and in vivo revealed that the generated subclones displayed characteristic and sustained features of tumour initiating cells as well as highly aggressive properties related to tumour progression and metastasis. These characteristics could clearly be correlated with the expression of CSC markers that might have prognostic value in the clinical HCC setting. Therefore, we conclude that our CSC enriched HepG2 clones certainly represent suitable model systems to study the role of CSCs during HCC initiation, progression and drug resistance. John Wiley and Sons Inc. 2018-10-02 2018-12 /pmc/articles/PMC6237557/ /pubmed/30280520 http://dx.doi.org/10.1111/jcmm.13911 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Muenzner, Julienne K. Kunze, Philipp Lindner, Pablo Polaschek, Sandra Menke, Kira Eckstein, Markus Geppert, Carol I. Chanvorachote, Pithi Baeuerle, Tobias Hartmann, Arndt Schneider‐Stock, Regine Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential |
title | Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential |
title_full | Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential |
title_fullStr | Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential |
title_full_unstemmed | Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential |
title_short | Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential |
title_sort | generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237557/ https://www.ncbi.nlm.nih.gov/pubmed/30280520 http://dx.doi.org/10.1111/jcmm.13911 |
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