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Young bone marrow Sca‐1 cells protect aged retina from ischaemia‐reperfusion injury through activation of FGF2

Retinal ganglion cell apoptosis and optic nerve degeneration are prevalent in aged patients, which may be related to the decrease in bone marrow (BM) stem cell number/function because of the possible cross‐talk between the two organs. This pathological process is accelerated by retinal ischaemia‐rep...

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Autores principales: Shao, Zhengbo, Wu, Jie, Du, Guoqing, Song, Huifang, Li, Shu‐Hong, He, Sheng, Li, Jiao, Wu, Jun, Weisel, Richard D., Yuan, Huiping, Li, Ren‐Ke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237572/
https://www.ncbi.nlm.nih.gov/pubmed/30255622
http://dx.doi.org/10.1111/jcmm.13905
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author Shao, Zhengbo
Wu, Jie
Du, Guoqing
Song, Huifang
Li, Shu‐Hong
He, Sheng
Li, Jiao
Wu, Jun
Weisel, Richard D.
Yuan, Huiping
Li, Ren‐Ke
author_facet Shao, Zhengbo
Wu, Jie
Du, Guoqing
Song, Huifang
Li, Shu‐Hong
He, Sheng
Li, Jiao
Wu, Jun
Weisel, Richard D.
Yuan, Huiping
Li, Ren‐Ke
author_sort Shao, Zhengbo
collection PubMed
description Retinal ganglion cell apoptosis and optic nerve degeneration are prevalent in aged patients, which may be related to the decrease in bone marrow (BM) stem cell number/function because of the possible cross‐talk between the two organs. This pathological process is accelerated by retinal ischaemia‐reperfusion (I/R) injury. This study investigated whether young BM stem cells can regenerate and repair the aged retina after acute I/R injury. Young BM stem cell antigen 1 positive (Sca‐1(+)) or Sca‐1(−) cells were transplanted into lethally irradiated aged recipient mice to generate Sca‐1(+) and Sca‐1(−) chimaeras, respectively. The animals were housed for 3 months to allow the young Sca‐1 cells to repopulate in the BM of aged mice. Retinal I/R was then induced by elevation of intraocular pressure. Better preservation of visual function was found in Sca‐1(+) than Sca‐1(−) chimaeras 7 days after injury. More Sca‐1(+) cells homed to the retina than Sca‐1(−) cells and more cells differentiated into glial and microglial cells in the Sca‐1(+) chimaeras. After injury, Sca‐1(+) cells in the retina reduced host cellular apoptosis, which was associated with higher expression of fibroblast growth factor 2 (FGF2) in the Sca‐1(+) chimaeras. Young Sca‐1(+) cells repopulated the stem cells in the aged retina and diminished cellular apoptosis after acute I/R injury through FGF2 and Akt signalling pathways.
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spelling pubmed-62375722018-12-01 Young bone marrow Sca‐1 cells protect aged retina from ischaemia‐reperfusion injury through activation of FGF2 Shao, Zhengbo Wu, Jie Du, Guoqing Song, Huifang Li, Shu‐Hong He, Sheng Li, Jiao Wu, Jun Weisel, Richard D. Yuan, Huiping Li, Ren‐Ke J Cell Mol Med Original Articles Retinal ganglion cell apoptosis and optic nerve degeneration are prevalent in aged patients, which may be related to the decrease in bone marrow (BM) stem cell number/function because of the possible cross‐talk between the two organs. This pathological process is accelerated by retinal ischaemia‐reperfusion (I/R) injury. This study investigated whether young BM stem cells can regenerate and repair the aged retina after acute I/R injury. Young BM stem cell antigen 1 positive (Sca‐1(+)) or Sca‐1(−) cells were transplanted into lethally irradiated aged recipient mice to generate Sca‐1(+) and Sca‐1(−) chimaeras, respectively. The animals were housed for 3 months to allow the young Sca‐1 cells to repopulate in the BM of aged mice. Retinal I/R was then induced by elevation of intraocular pressure. Better preservation of visual function was found in Sca‐1(+) than Sca‐1(−) chimaeras 7 days after injury. More Sca‐1(+) cells homed to the retina than Sca‐1(−) cells and more cells differentiated into glial and microglial cells in the Sca‐1(+) chimaeras. After injury, Sca‐1(+) cells in the retina reduced host cellular apoptosis, which was associated with higher expression of fibroblast growth factor 2 (FGF2) in the Sca‐1(+) chimaeras. Young Sca‐1(+) cells repopulated the stem cells in the aged retina and diminished cellular apoptosis after acute I/R injury through FGF2 and Akt signalling pathways. John Wiley and Sons Inc. 2018-09-25 2018-12 /pmc/articles/PMC6237572/ /pubmed/30255622 http://dx.doi.org/10.1111/jcmm.13905 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Shao, Zhengbo
Wu, Jie
Du, Guoqing
Song, Huifang
Li, Shu‐Hong
He, Sheng
Li, Jiao
Wu, Jun
Weisel, Richard D.
Yuan, Huiping
Li, Ren‐Ke
Young bone marrow Sca‐1 cells protect aged retina from ischaemia‐reperfusion injury through activation of FGF2
title Young bone marrow Sca‐1 cells protect aged retina from ischaemia‐reperfusion injury through activation of FGF2
title_full Young bone marrow Sca‐1 cells protect aged retina from ischaemia‐reperfusion injury through activation of FGF2
title_fullStr Young bone marrow Sca‐1 cells protect aged retina from ischaemia‐reperfusion injury through activation of FGF2
title_full_unstemmed Young bone marrow Sca‐1 cells protect aged retina from ischaemia‐reperfusion injury through activation of FGF2
title_short Young bone marrow Sca‐1 cells protect aged retina from ischaemia‐reperfusion injury through activation of FGF2
title_sort young bone marrow sca‐1 cells protect aged retina from ischaemia‐reperfusion injury through activation of fgf2
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237572/
https://www.ncbi.nlm.nih.gov/pubmed/30255622
http://dx.doi.org/10.1111/jcmm.13905
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