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Follistatin‐like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase‐dependent apoptosis and regulating Bcl‐2 family proteins
Hepatocellular carcinoma (HCC) is one of the most common and deadly malignant tumors in the world, especially in China. Follistatin‐like protein 5 (FSTL5) is a member of the FSTL family, which is involved in cell proliferation, migration, differentiation, and embryo development. We aimed to investig...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237577/ https://www.ncbi.nlm.nih.gov/pubmed/30255547 http://dx.doi.org/10.1111/jcmm.13906 |
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author | Li, Chunlei Dai, Lei Zhang, Junfeng Zhang, Yujing Lin, Yi Cheng, Lin Tian, Hongwei Zhang, Xin Wang, Qingnan Yang, Qianmei Wang, Yuan Shi, Gang Cheng, Fuyi Su, Xiaolan Yang, Yang Zhang, Shuang Yu, Dechao Wei, Yuquan Deng, Hongxin |
author_facet | Li, Chunlei Dai, Lei Zhang, Junfeng Zhang, Yujing Lin, Yi Cheng, Lin Tian, Hongwei Zhang, Xin Wang, Qingnan Yang, Qianmei Wang, Yuan Shi, Gang Cheng, Fuyi Su, Xiaolan Yang, Yang Zhang, Shuang Yu, Dechao Wei, Yuquan Deng, Hongxin |
author_sort | Li, Chunlei |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is one of the most common and deadly malignant tumors in the world, especially in China. Follistatin‐like protein 5 (FSTL5) is a member of the FSTL family, which is involved in cell proliferation, migration, differentiation, and embryo development. We aimed to investigate the function and underlying mechanism of FSTL5 in HCC. FSTL5 expression was determined by immunohistochemistry staining in a liver cancer tissue microarray (TMA) and the correlation between FSTL5 and the prognosis of HCC patients was analysed. Further proliferation assay, colony formation assay, flow cytometry, and xenograft tumor model were performed to investigate the bioeffects of FSTL5 in HCC in vitro and in vivo. We found that FSTL5 expression was downregulated in HCC tissues and positively correlated with the prognosis of patients with HCC at tumor node metastasis stage I/II. Overexpression of FSTL5 efficiently impaired HCC growth both in vivo and in vitro with an exogenous manner. Mechanistic investigation demonstrated that FSTL5 promoted HCC cell apoptosis in a caspase‐dependent manner and regulated Bcl‐2 family proteins. These results indicate that FSTL5 may be a potential novel target for HCC treatment, and a biomarker for tumor prognosis. |
format | Online Article Text |
id | pubmed-6237577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62375772018-12-01 Follistatin‐like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase‐dependent apoptosis and regulating Bcl‐2 family proteins Li, Chunlei Dai, Lei Zhang, Junfeng Zhang, Yujing Lin, Yi Cheng, Lin Tian, Hongwei Zhang, Xin Wang, Qingnan Yang, Qianmei Wang, Yuan Shi, Gang Cheng, Fuyi Su, Xiaolan Yang, Yang Zhang, Shuang Yu, Dechao Wei, Yuquan Deng, Hongxin J Cell Mol Med Original Articles Hepatocellular carcinoma (HCC) is one of the most common and deadly malignant tumors in the world, especially in China. Follistatin‐like protein 5 (FSTL5) is a member of the FSTL family, which is involved in cell proliferation, migration, differentiation, and embryo development. We aimed to investigate the function and underlying mechanism of FSTL5 in HCC. FSTL5 expression was determined by immunohistochemistry staining in a liver cancer tissue microarray (TMA) and the correlation between FSTL5 and the prognosis of HCC patients was analysed. Further proliferation assay, colony formation assay, flow cytometry, and xenograft tumor model were performed to investigate the bioeffects of FSTL5 in HCC in vitro and in vivo. We found that FSTL5 expression was downregulated in HCC tissues and positively correlated with the prognosis of patients with HCC at tumor node metastasis stage I/II. Overexpression of FSTL5 efficiently impaired HCC growth both in vivo and in vitro with an exogenous manner. Mechanistic investigation demonstrated that FSTL5 promoted HCC cell apoptosis in a caspase‐dependent manner and regulated Bcl‐2 family proteins. These results indicate that FSTL5 may be a potential novel target for HCC treatment, and a biomarker for tumor prognosis. John Wiley and Sons Inc. 2018-09-25 2018-12 /pmc/articles/PMC6237577/ /pubmed/30255547 http://dx.doi.org/10.1111/jcmm.13906 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Li, Chunlei Dai, Lei Zhang, Junfeng Zhang, Yujing Lin, Yi Cheng, Lin Tian, Hongwei Zhang, Xin Wang, Qingnan Yang, Qianmei Wang, Yuan Shi, Gang Cheng, Fuyi Su, Xiaolan Yang, Yang Zhang, Shuang Yu, Dechao Wei, Yuquan Deng, Hongxin Follistatin‐like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase‐dependent apoptosis and regulating Bcl‐2 family proteins |
title | Follistatin‐like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase‐dependent apoptosis and regulating Bcl‐2 family proteins |
title_full | Follistatin‐like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase‐dependent apoptosis and regulating Bcl‐2 family proteins |
title_fullStr | Follistatin‐like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase‐dependent apoptosis and regulating Bcl‐2 family proteins |
title_full_unstemmed | Follistatin‐like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase‐dependent apoptosis and regulating Bcl‐2 family proteins |
title_short | Follistatin‐like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase‐dependent apoptosis and regulating Bcl‐2 family proteins |
title_sort | follistatin‐like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase‐dependent apoptosis and regulating bcl‐2 family proteins |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237577/ https://www.ncbi.nlm.nih.gov/pubmed/30255547 http://dx.doi.org/10.1111/jcmm.13906 |
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