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Selenium‐sensitive miRNA‐181a‐5p targeting SBP2 regulates selenoproteins expression in cartilage

Selenium (Se) deficiency brings about defects in the biosynthesis of several selenoproteins and has been associated with aberrant chondrogenesis. Selenocysteine (Sec) Insertion Sequence (SECIS) and SECIS binding protein 2 (SBP2) interaction is a very critical node for the metabolic balance between S...

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Autores principales: Min, Zixin, Guo, Yuanxu, Sun, Mengyao, Hussain, Safdar, Zhao, Yitong, Guo, Dongxian, Huang, Huang, Heng, Lisong, Zhang, Fujun, Ning, Qilan, Han, Yan, Xu, Peng, Zhong, Nannan, Sun, Jian, Lu, Shemin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237606/
https://www.ncbi.nlm.nih.gov/pubmed/30247797
http://dx.doi.org/10.1111/jcmm.13858
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author Min, Zixin
Guo, Yuanxu
Sun, Mengyao
Hussain, Safdar
Zhao, Yitong
Guo, Dongxian
Huang, Huang
Heng, Lisong
Zhang, Fujun
Ning, Qilan
Han, Yan
Xu, Peng
Zhong, Nannan
Sun, Jian
Lu, Shemin
author_facet Min, Zixin
Guo, Yuanxu
Sun, Mengyao
Hussain, Safdar
Zhao, Yitong
Guo, Dongxian
Huang, Huang
Heng, Lisong
Zhang, Fujun
Ning, Qilan
Han, Yan
Xu, Peng
Zhong, Nannan
Sun, Jian
Lu, Shemin
author_sort Min, Zixin
collection PubMed
description Selenium (Se) deficiency brings about defects in the biosynthesis of several selenoproteins and has been associated with aberrant chondrogenesis. Selenocysteine (Sec) Insertion Sequence (SECIS) and SECIS binding protein 2 (SBP2) interaction is a very critical node for the metabolic balance between Se and selenoproteins. The Gpx1, Gpx4 and SelS have different binding affinities with SBP2 in cells. According to our results, both miR‐181a‐5p and SBP2 appeared to be selenium‐sensitive and regulated the expression of selenoproteins in C28/I2 cells under Se sufficient environment. However, they showed significantly opposite expression trend in Se deficiency rats cartilage and SeD C28/I2 cells. The SBP2 is a direct target gene of miR‐181a‐5p in C28/I2 cells as determined by reporter gene and off‐target experiments. And the miR‐181a‐5p could regulate SBP2 and the selenoproteins in C28/I2 cells. Depending upon the Se supply levels, C28/I2 cells were divided into three groups, that is normal Se, SeD and SeS, which underwent through a 7‐day Se deprivation process, then SBP2 was knocked‐down and overexpressed in all the groups. Moreover, the selected selenoproteins were down‐regulated in second‐generation low Se diet rat cartilage. The selenoproteins expression was decreased by Se deficiency which depended on the Selenium‐sensitive miR‐181a‐5p to participate and regulate SBP2 at post‐transcriptional level. It involves a series of antioxidant and ECM (extracellular matrix) genes, to overcome the ROS‐related stress for the protection of essential physiological functions and to maintain the balance between anabolism and catabolism of the cartilage.
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spelling pubmed-62376062018-12-01 Selenium‐sensitive miRNA‐181a‐5p targeting SBP2 regulates selenoproteins expression in cartilage Min, Zixin Guo, Yuanxu Sun, Mengyao Hussain, Safdar Zhao, Yitong Guo, Dongxian Huang, Huang Heng, Lisong Zhang, Fujun Ning, Qilan Han, Yan Xu, Peng Zhong, Nannan Sun, Jian Lu, Shemin J Cell Mol Med Original Articles Selenium (Se) deficiency brings about defects in the biosynthesis of several selenoproteins and has been associated with aberrant chondrogenesis. Selenocysteine (Sec) Insertion Sequence (SECIS) and SECIS binding protein 2 (SBP2) interaction is a very critical node for the metabolic balance between Se and selenoproteins. The Gpx1, Gpx4 and SelS have different binding affinities with SBP2 in cells. According to our results, both miR‐181a‐5p and SBP2 appeared to be selenium‐sensitive and regulated the expression of selenoproteins in C28/I2 cells under Se sufficient environment. However, they showed significantly opposite expression trend in Se deficiency rats cartilage and SeD C28/I2 cells. The SBP2 is a direct target gene of miR‐181a‐5p in C28/I2 cells as determined by reporter gene and off‐target experiments. And the miR‐181a‐5p could regulate SBP2 and the selenoproteins in C28/I2 cells. Depending upon the Se supply levels, C28/I2 cells were divided into three groups, that is normal Se, SeD and SeS, which underwent through a 7‐day Se deprivation process, then SBP2 was knocked‐down and overexpressed in all the groups. Moreover, the selected selenoproteins were down‐regulated in second‐generation low Se diet rat cartilage. The selenoproteins expression was decreased by Se deficiency which depended on the Selenium‐sensitive miR‐181a‐5p to participate and regulate SBP2 at post‐transcriptional level. It involves a series of antioxidant and ECM (extracellular matrix) genes, to overcome the ROS‐related stress for the protection of essential physiological functions and to maintain the balance between anabolism and catabolism of the cartilage. John Wiley and Sons Inc. 2018-09-24 2018-12 /pmc/articles/PMC6237606/ /pubmed/30247797 http://dx.doi.org/10.1111/jcmm.13858 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Min, Zixin
Guo, Yuanxu
Sun, Mengyao
Hussain, Safdar
Zhao, Yitong
Guo, Dongxian
Huang, Huang
Heng, Lisong
Zhang, Fujun
Ning, Qilan
Han, Yan
Xu, Peng
Zhong, Nannan
Sun, Jian
Lu, Shemin
Selenium‐sensitive miRNA‐181a‐5p targeting SBP2 regulates selenoproteins expression in cartilage
title Selenium‐sensitive miRNA‐181a‐5p targeting SBP2 regulates selenoproteins expression in cartilage
title_full Selenium‐sensitive miRNA‐181a‐5p targeting SBP2 regulates selenoproteins expression in cartilage
title_fullStr Selenium‐sensitive miRNA‐181a‐5p targeting SBP2 regulates selenoproteins expression in cartilage
title_full_unstemmed Selenium‐sensitive miRNA‐181a‐5p targeting SBP2 regulates selenoproteins expression in cartilage
title_short Selenium‐sensitive miRNA‐181a‐5p targeting SBP2 regulates selenoproteins expression in cartilage
title_sort selenium‐sensitive mirna‐181a‐5p targeting sbp2 regulates selenoproteins expression in cartilage
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237606/
https://www.ncbi.nlm.nih.gov/pubmed/30247797
http://dx.doi.org/10.1111/jcmm.13858
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