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Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis

Emerging evidence have illustrated the vital roles of long noncoding RNAs (lncRNAs) in glioma. Nevertheless, the majority of their roles and mechanisms in gliomagenesis are still largely unclear. In this study, we investigate the roles of lncRNA CASC9 on glioma tumourigenesis and authenticate its po...

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Autores principales: Liu, Hongjiang, Li, Chen, Yang, Jiankai, Sun, Yuchen, Zhang, Shunyao, Yang, Jipeng, Yang, Liang, Wang, Yuanyu, Jiao, Baohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237610/
https://www.ncbi.nlm.nih.gov/pubmed/30270508
http://dx.doi.org/10.1111/jcmm.13932
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author Liu, Hongjiang
Li, Chen
Yang, Jiankai
Sun, Yuchen
Zhang, Shunyao
Yang, Jipeng
Yang, Liang
Wang, Yuanyu
Jiao, Baohua
author_facet Liu, Hongjiang
Li, Chen
Yang, Jiankai
Sun, Yuchen
Zhang, Shunyao
Yang, Jipeng
Yang, Liang
Wang, Yuanyu
Jiao, Baohua
author_sort Liu, Hongjiang
collection PubMed
description Emerging evidence have illustrated the vital roles of long noncoding RNAs (lncRNAs) in glioma. Nevertheless, the majority of their roles and mechanisms in gliomagenesis are still largely unclear. In this study, we investigate the roles of lncRNA CASC9 on glioma tumourigenesis and authenticate its potential mechanisms. Results manifested that CASC9 was highly expressed in glioma specimens and cells, moreover, the ectopic overexpression was correlated with glioma patients’ clinic. Functional studies found that siRNA‐mediated CASC9 silencing inhibited the proliferative ability, invasion in vitro, and impaired the tumour growth in vivo. Mechanical studies revealed that miR‐519d both targeted the 3′‐UTR of CASC9 and STAT3 mRNA, which was identified by luciferase reporter assay and RNA immunoprecipitation (RIP). Moreover, chromatin immunoprecipitation (ChIP) and luciferase reporter assay revealed that STAT3, an oncogenic transcription factor, could bind with the promoter of CASC9 and activate its transcriptional level. In conclusion, our results concluded that CASC9 promotes STAT3 expression via sponging miR‐519d, in return, STAT3 activate CASC9 transcription, forming a positive feedback loop of CASC9/miR‐519d/STAT3. The novel finding provides a potential therapeutic target for glioma.
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spelling pubmed-62376102018-12-01 Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis Liu, Hongjiang Li, Chen Yang, Jiankai Sun, Yuchen Zhang, Shunyao Yang, Jipeng Yang, Liang Wang, Yuanyu Jiao, Baohua J Cell Mol Med Original Articles Emerging evidence have illustrated the vital roles of long noncoding RNAs (lncRNAs) in glioma. Nevertheless, the majority of their roles and mechanisms in gliomagenesis are still largely unclear. In this study, we investigate the roles of lncRNA CASC9 on glioma tumourigenesis and authenticate its potential mechanisms. Results manifested that CASC9 was highly expressed in glioma specimens and cells, moreover, the ectopic overexpression was correlated with glioma patients’ clinic. Functional studies found that siRNA‐mediated CASC9 silencing inhibited the proliferative ability, invasion in vitro, and impaired the tumour growth in vivo. Mechanical studies revealed that miR‐519d both targeted the 3′‐UTR of CASC9 and STAT3 mRNA, which was identified by luciferase reporter assay and RNA immunoprecipitation (RIP). Moreover, chromatin immunoprecipitation (ChIP) and luciferase reporter assay revealed that STAT3, an oncogenic transcription factor, could bind with the promoter of CASC9 and activate its transcriptional level. In conclusion, our results concluded that CASC9 promotes STAT3 expression via sponging miR‐519d, in return, STAT3 activate CASC9 transcription, forming a positive feedback loop of CASC9/miR‐519d/STAT3. The novel finding provides a potential therapeutic target for glioma. John Wiley and Sons Inc. 2018-09-30 2018-12 /pmc/articles/PMC6237610/ /pubmed/30270508 http://dx.doi.org/10.1111/jcmm.13932 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Liu, Hongjiang
Li, Chen
Yang, Jiankai
Sun, Yuchen
Zhang, Shunyao
Yang, Jipeng
Yang, Liang
Wang, Yuanyu
Jiao, Baohua
Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis
title Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis
title_full Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis
title_fullStr Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis
title_full_unstemmed Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis
title_short Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis
title_sort long noncoding rna casc9/mir‐519d/stat3 positive feedback loop facilitate the glioma tumourigenesis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237610/
https://www.ncbi.nlm.nih.gov/pubmed/30270508
http://dx.doi.org/10.1111/jcmm.13932
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