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Computational imaging of aortic vasa vasorum and neovascularization in rabbits using contrast-enhanced intravascular ultrasound: Association with histological analysis

OBJECTIVE: Neoangiogenesis is pathophysiologically related to atherosclerotic plaque growth and vulnerability. We examined the in vivo performance of a computational method using contrast-enhanced intravascular ultrasound (CE-IVUS) to detect and quantify aortic wall neovascularization in rabbits. We...

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Autores principales: Vavuranakis, Manolis, Papaioannou, Theodore G., Vrachatis, Dimitrios, Katsimboulas, Michael, Sanidas, Elias A., Vaina, Sophia, Agrogiannis, George, Patsouris, Efstratios, Kakadiaris, Ioannis, Stefanadis, Christodoulos, Tousoulis, Dimitrios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kare Publishing 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237958/
https://www.ncbi.nlm.nih.gov/pubmed/30088486
http://dx.doi.org/10.14744/AnatolJCardiol.2018.35761
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author Vavuranakis, Manolis
Papaioannou, Theodore G.
Vrachatis, Dimitrios
Katsimboulas, Michael
Sanidas, Elias A.
Vaina, Sophia
Agrogiannis, George
Patsouris, Efstratios
Kakadiaris, Ioannis
Stefanadis, Christodoulos
Tousoulis, Dimitrios
author_facet Vavuranakis, Manolis
Papaioannou, Theodore G.
Vrachatis, Dimitrios
Katsimboulas, Michael
Sanidas, Elias A.
Vaina, Sophia
Agrogiannis, George
Patsouris, Efstratios
Kakadiaris, Ioannis
Stefanadis, Christodoulos
Tousoulis, Dimitrios
author_sort Vavuranakis, Manolis
collection PubMed
description OBJECTIVE: Neoangiogenesis is pathophysiologically related to atherosclerotic plaque growth and vulnerability. We examined the in vivo performance of a computational method using contrast-enhanced intravascular ultrasound (CE-IVUS) to detect and quantify aortic wall neovascularization in rabbits. We also compared these findings with histological data. METHODS: Nine rabbits were fed with a hyperlipidemic diet. IVUS image sequences were continuously recorded before and after the injection of a contrast agent. Mean enhancement of intensity of a region of interest (MEIR) was calculated using differential imaging algorithm. The percent difference of MEIR before and after the injection of microbubbles (d_MEIR) was used as an index of the density of plaque or/and adventitial neovascularization. Aortic segments were excised for histological analysis. RESULTS: CE-IVUS and histological analysis were performed in 11 arterial segments. MEIR was significantly increased (~20%) after microbubble injection (from 8.1±0.9 to 9.7±1.8, p=0.016). Segments with increased VV/neovessels in the tunica adventitia (histological scores 2 and 3) had significantly higher d_MEIR compared with segments with low presence of VV/neovessels (score 1); 40.5±22.9 vs. 8±14.6, p=0.024, respectively. CONCLUSION: It is possible to detect VV or neovessels in vivo using computational analysis of CE-IVUS images, which is in agreement with histological data. These findings may have critical implications on vulnerable plaque assessment and risk stratification.
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spelling pubmed-62379582018-11-19 Computational imaging of aortic vasa vasorum and neovascularization in rabbits using contrast-enhanced intravascular ultrasound: Association with histological analysis Vavuranakis, Manolis Papaioannou, Theodore G. Vrachatis, Dimitrios Katsimboulas, Michael Sanidas, Elias A. Vaina, Sophia Agrogiannis, George Patsouris, Efstratios Kakadiaris, Ioannis Stefanadis, Christodoulos Tousoulis, Dimitrios Anatol J Cardiol Original Investigation OBJECTIVE: Neoangiogenesis is pathophysiologically related to atherosclerotic plaque growth and vulnerability. We examined the in vivo performance of a computational method using contrast-enhanced intravascular ultrasound (CE-IVUS) to detect and quantify aortic wall neovascularization in rabbits. We also compared these findings with histological data. METHODS: Nine rabbits were fed with a hyperlipidemic diet. IVUS image sequences were continuously recorded before and after the injection of a contrast agent. Mean enhancement of intensity of a region of interest (MEIR) was calculated using differential imaging algorithm. The percent difference of MEIR before and after the injection of microbubbles (d_MEIR) was used as an index of the density of plaque or/and adventitial neovascularization. Aortic segments were excised for histological analysis. RESULTS: CE-IVUS and histological analysis were performed in 11 arterial segments. MEIR was significantly increased (~20%) after microbubble injection (from 8.1±0.9 to 9.7±1.8, p=0.016). Segments with increased VV/neovessels in the tunica adventitia (histological scores 2 and 3) had significantly higher d_MEIR compared with segments with low presence of VV/neovessels (score 1); 40.5±22.9 vs. 8±14.6, p=0.024, respectively. CONCLUSION: It is possible to detect VV or neovessels in vivo using computational analysis of CE-IVUS images, which is in agreement with histological data. These findings may have critical implications on vulnerable plaque assessment and risk stratification. Kare Publishing 2018-08 2018-07-27 /pmc/articles/PMC6237958/ /pubmed/30088486 http://dx.doi.org/10.14744/AnatolJCardiol.2018.35761 Text en Copyright: © 2018 Turkish Society of Cardiology http://creativecommons.org/licenses/by-nc-sa/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Original Investigation
Vavuranakis, Manolis
Papaioannou, Theodore G.
Vrachatis, Dimitrios
Katsimboulas, Michael
Sanidas, Elias A.
Vaina, Sophia
Agrogiannis, George
Patsouris, Efstratios
Kakadiaris, Ioannis
Stefanadis, Christodoulos
Tousoulis, Dimitrios
Computational imaging of aortic vasa vasorum and neovascularization in rabbits using contrast-enhanced intravascular ultrasound: Association with histological analysis
title Computational imaging of aortic vasa vasorum and neovascularization in rabbits using contrast-enhanced intravascular ultrasound: Association with histological analysis
title_full Computational imaging of aortic vasa vasorum and neovascularization in rabbits using contrast-enhanced intravascular ultrasound: Association with histological analysis
title_fullStr Computational imaging of aortic vasa vasorum and neovascularization in rabbits using contrast-enhanced intravascular ultrasound: Association with histological analysis
title_full_unstemmed Computational imaging of aortic vasa vasorum and neovascularization in rabbits using contrast-enhanced intravascular ultrasound: Association with histological analysis
title_short Computational imaging of aortic vasa vasorum and neovascularization in rabbits using contrast-enhanced intravascular ultrasound: Association with histological analysis
title_sort computational imaging of aortic vasa vasorum and neovascularization in rabbits using contrast-enhanced intravascular ultrasound: association with histological analysis
topic Original Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237958/
https://www.ncbi.nlm.nih.gov/pubmed/30088486
http://dx.doi.org/10.14744/AnatolJCardiol.2018.35761
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