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The severity of LPS induced inflammatory injury is negatively associated with the functional liver mass after LPS injection in rat model

BACKGROUND: High levels of serum lipopolysaccharide (LPS) were observed in sepsis patients with liver injury and high mortality. However, the role of liver in modulation LPS induced inflammatory injury was ill investigated. In the present study, the severity of LPS induced inflammatory response was...

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Autores principales: Fang, Haoshu, Liu, Anding, Chen, Xulin, Cheng, Wenhui, Dirsch, Olaf, Dahmen, Uta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238277/
https://www.ncbi.nlm.nih.gov/pubmed/30473633
http://dx.doi.org/10.1186/s12950-018-0197-4
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author Fang, Haoshu
Liu, Anding
Chen, Xulin
Cheng, Wenhui
Dirsch, Olaf
Dahmen, Uta
author_facet Fang, Haoshu
Liu, Anding
Chen, Xulin
Cheng, Wenhui
Dirsch, Olaf
Dahmen, Uta
author_sort Fang, Haoshu
collection PubMed
description BACKGROUND: High levels of serum lipopolysaccharide (LPS) were observed in sepsis patients with liver injury and high mortality. However, the role of liver in modulation LPS induced inflammatory injury was ill investigated. In the present study, the severity of LPS induced inflammatory response was observed after liver resection or portal branch occlusion to decreasing functional liver mass. The local and systemic damage was observed to investigate the role of liver in modulation inflammatory injury. METHODS: First, 30%, 70%, and 90% partial hepatectomy (PH) were performed, and serum TNF-α, survival rate, and hepatic LPS uptake was observed. Second, LPS-exposure of the functional liver mass was decreased by selectively blocking the RL prior to LPS-injection, which was given 30 min before a 70% PH, and the inflammatory response was compared in the occluded and the non-occluded liver. The control group was subjected to LPS injection 30 min prior to liver resection without blocking the RL transiently. The serum TNF-α, ALT, AST, creatinine levels, and urea levels, survival rate, hepatic LPS uptake, and hepatic inflammatory cytokines was observed. RESULTS: The decreasing of functional liver mass after 90%, 70%, and 30% PH was associated with decreased serum TNF-α, survival rate, and increased hepatic LPS uptake after LPS injection. Occluding the right lobes (RL) prior to LPS administration reversed the liver injury caused by 70% PH, indicated by 100% survival rate and decreased liver and kidney injury, and systemic inflammatory response. The induction of inflammatory response in occluding liver lobes were lower than un-occluding liver lobes. CONCLUSIONS: The severity of the LPS-induced systemic inflammatory injury is determined by functional liver volume. This observation suggests that the liver is the central organ for the initiation of the inflammatory response, and is involved in causing a severe SIRS with systemic damage and death. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12950-018-0197-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-62382772018-11-23 The severity of LPS induced inflammatory injury is negatively associated with the functional liver mass after LPS injection in rat model Fang, Haoshu Liu, Anding Chen, Xulin Cheng, Wenhui Dirsch, Olaf Dahmen, Uta J Inflamm (Lond) Research BACKGROUND: High levels of serum lipopolysaccharide (LPS) were observed in sepsis patients with liver injury and high mortality. However, the role of liver in modulation LPS induced inflammatory injury was ill investigated. In the present study, the severity of LPS induced inflammatory response was observed after liver resection or portal branch occlusion to decreasing functional liver mass. The local and systemic damage was observed to investigate the role of liver in modulation inflammatory injury. METHODS: First, 30%, 70%, and 90% partial hepatectomy (PH) were performed, and serum TNF-α, survival rate, and hepatic LPS uptake was observed. Second, LPS-exposure of the functional liver mass was decreased by selectively blocking the RL prior to LPS-injection, which was given 30 min before a 70% PH, and the inflammatory response was compared in the occluded and the non-occluded liver. The control group was subjected to LPS injection 30 min prior to liver resection without blocking the RL transiently. The serum TNF-α, ALT, AST, creatinine levels, and urea levels, survival rate, hepatic LPS uptake, and hepatic inflammatory cytokines was observed. RESULTS: The decreasing of functional liver mass after 90%, 70%, and 30% PH was associated with decreased serum TNF-α, survival rate, and increased hepatic LPS uptake after LPS injection. Occluding the right lobes (RL) prior to LPS administration reversed the liver injury caused by 70% PH, indicated by 100% survival rate and decreased liver and kidney injury, and systemic inflammatory response. The induction of inflammatory response in occluding liver lobes were lower than un-occluding liver lobes. CONCLUSIONS: The severity of the LPS-induced systemic inflammatory injury is determined by functional liver volume. This observation suggests that the liver is the central organ for the initiation of the inflammatory response, and is involved in causing a severe SIRS with systemic damage and death. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12950-018-0197-4) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-15 /pmc/articles/PMC6238277/ /pubmed/30473633 http://dx.doi.org/10.1186/s12950-018-0197-4 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Fang, Haoshu
Liu, Anding
Chen, Xulin
Cheng, Wenhui
Dirsch, Olaf
Dahmen, Uta
The severity of LPS induced inflammatory injury is negatively associated with the functional liver mass after LPS injection in rat model
title The severity of LPS induced inflammatory injury is negatively associated with the functional liver mass after LPS injection in rat model
title_full The severity of LPS induced inflammatory injury is negatively associated with the functional liver mass after LPS injection in rat model
title_fullStr The severity of LPS induced inflammatory injury is negatively associated with the functional liver mass after LPS injection in rat model
title_full_unstemmed The severity of LPS induced inflammatory injury is negatively associated with the functional liver mass after LPS injection in rat model
title_short The severity of LPS induced inflammatory injury is negatively associated with the functional liver mass after LPS injection in rat model
title_sort severity of lps induced inflammatory injury is negatively associated with the functional liver mass after lps injection in rat model
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238277/
https://www.ncbi.nlm.nih.gov/pubmed/30473633
http://dx.doi.org/10.1186/s12950-018-0197-4
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