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Proteins of generalist and specialist pathogens differ in their amino acid composition

Pathogens differ in their host specificities, with species infecting a unique host (specialist pathogens) and others having a wide host range (generalists). Molecular determinants of pathogen’s host range remain poorly understood. Secreted proteins of generalist pathogens are expected to have a broa...

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Autores principales: Blanco, Luz P, Payne, Bryan L, Feyertag, Felix, Alvarez-Ponce, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238412/
https://www.ncbi.nlm.nih.gov/pubmed/30456362
http://dx.doi.org/10.26508/lsa.201800017
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author Blanco, Luz P
Payne, Bryan L
Feyertag, Felix
Alvarez-Ponce, David
author_facet Blanco, Luz P
Payne, Bryan L
Feyertag, Felix
Alvarez-Ponce, David
author_sort Blanco, Luz P
collection PubMed
description Pathogens differ in their host specificities, with species infecting a unique host (specialist pathogens) and others having a wide host range (generalists). Molecular determinants of pathogen’s host range remain poorly understood. Secreted proteins of generalist pathogens are expected to have a broader range of intermolecular interactions (i.e., higher promiscuity) compared with their specialist counterparts. We hypothesize that this increased promiscuity of generalist secretomes may be based on an elevated content of primitive amino acids and intrinsically disordered regions, as these features are known to increase protein flexibility and interactivity. Here, we measure the proportion of primitive amino acids and percentage of intrinsically disordered residues in secreted, membrane, and cytoplasmic proteins from pathogens with different host specificity. Supporting our prediction, there is a significant general enrichment for primitive amino acids and intrinsically disordered regions in proteins from generalists compared to specialists, particularly among secreted proteins in prokaryotes. Our findings support our hypothesis that secreted proteins' amino acid composition and disordered content influence the pathogens' host range.
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spelling pubmed-62384122018-11-19 Proteins of generalist and specialist pathogens differ in their amino acid composition Blanco, Luz P Payne, Bryan L Feyertag, Felix Alvarez-Ponce, David Life Sci Alliance Research Articles Pathogens differ in their host specificities, with species infecting a unique host (specialist pathogens) and others having a wide host range (generalists). Molecular determinants of pathogen’s host range remain poorly understood. Secreted proteins of generalist pathogens are expected to have a broader range of intermolecular interactions (i.e., higher promiscuity) compared with their specialist counterparts. We hypothesize that this increased promiscuity of generalist secretomes may be based on an elevated content of primitive amino acids and intrinsically disordered regions, as these features are known to increase protein flexibility and interactivity. Here, we measure the proportion of primitive amino acids and percentage of intrinsically disordered residues in secreted, membrane, and cytoplasmic proteins from pathogens with different host specificity. Supporting our prediction, there is a significant general enrichment for primitive amino acids and intrinsically disordered regions in proteins from generalists compared to specialists, particularly among secreted proteins in prokaryotes. Our findings support our hypothesis that secreted proteins' amino acid composition and disordered content influence the pathogens' host range. Life Science Alliance LLC 2018-07-17 /pmc/articles/PMC6238412/ /pubmed/30456362 http://dx.doi.org/10.26508/lsa.201800017 Text en © 2018 Blanco et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Blanco, Luz P
Payne, Bryan L
Feyertag, Felix
Alvarez-Ponce, David
Proteins of generalist and specialist pathogens differ in their amino acid composition
title Proteins of generalist and specialist pathogens differ in their amino acid composition
title_full Proteins of generalist and specialist pathogens differ in their amino acid composition
title_fullStr Proteins of generalist and specialist pathogens differ in their amino acid composition
title_full_unstemmed Proteins of generalist and specialist pathogens differ in their amino acid composition
title_short Proteins of generalist and specialist pathogens differ in their amino acid composition
title_sort proteins of generalist and specialist pathogens differ in their amino acid composition
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238412/
https://www.ncbi.nlm.nih.gov/pubmed/30456362
http://dx.doi.org/10.26508/lsa.201800017
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