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Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle
Several nucleic acid-based assays have been developed for detecting Anaplasma marginale and Anaplasma centrale in vectors and hosts, making the choice of method to use in endemic areas difficult. We evaluated the ability of the reverse line blot (RLB) hybridisation assay, two nested polymerase chain...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AOSIS
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238773/ https://www.ncbi.nlm.nih.gov/pubmed/28155283 http://dx.doi.org/10.4102/ojvr.v84i1.1262 |
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author | Chaisi, Mamohale E. Baxter, Janine R. Hove, Paidashe Choopa, Chimvwele N. Oosthuizen, Marinda C. Brayton, Kelly A. Khumalo, Zamantungwa T.H. Mutshembele, Awelani M. Mtshali, Moses S. Collins, Nicola E. |
author_facet | Chaisi, Mamohale E. Baxter, Janine R. Hove, Paidashe Choopa, Chimvwele N. Oosthuizen, Marinda C. Brayton, Kelly A. Khumalo, Zamantungwa T.H. Mutshembele, Awelani M. Mtshali, Moses S. Collins, Nicola E. |
author_sort | Chaisi, Mamohale E. |
collection | PubMed |
description | Several nucleic acid-based assays have been developed for detecting Anaplasma marginale and Anaplasma centrale in vectors and hosts, making the choice of method to use in endemic areas difficult. We evaluated the ability of the reverse line blot (RLB) hybridisation assay, two nested polymerase chain reaction (nPCR) assays and a duplex real-time quantitative polymerase chain reaction (qPCR) assay to detect A. marginale and A. centrale infections in cattle (n = 66) in South Africa. The lowest detection limits for A. marginale plasmid DNA were 2500 copies by the RLB assay, 250 copies by the nPCR and qPCR assays and 2500, 250 and 25 copies of A. centrale plasmid DNA by the RLB, nPCR and qPCR assays respectively. The qPCR assay detected more A. marginale- and A. centrale-positive samples than the other assays, either as single or mixed infections. Although the results of the qPCR and nPCR tests were in agreement for the majority (38) of A. marginale-positive samples, 13 samples tested negative for A. marginale using nPCR but positive using qPCR. To explain this discrepancy, the target sequence region of the nPCR assay was evaluated by cloning and sequencing the msp1β gene from selected field samples. The results indicated sequence variation in the internal forward primer (AM100) area amongst the South African A. marginale msp1β sequences, resulting in false negatives. We propose the use of the duplex qPCR assay in future studies as it is more sensitive and offers the benefits of quantification and multiplex detection of both Anaplasma spp. |
format | Online Article Text |
id | pubmed-6238773 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | AOSIS |
record_format | MEDLINE/PubMed |
spelling | pubmed-62387732018-11-26 Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle Chaisi, Mamohale E. Baxter, Janine R. Hove, Paidashe Choopa, Chimvwele N. Oosthuizen, Marinda C. Brayton, Kelly A. Khumalo, Zamantungwa T.H. Mutshembele, Awelani M. Mtshali, Moses S. Collins, Nicola E. Onderstepoort J Vet Res Original Research Several nucleic acid-based assays have been developed for detecting Anaplasma marginale and Anaplasma centrale in vectors and hosts, making the choice of method to use in endemic areas difficult. We evaluated the ability of the reverse line blot (RLB) hybridisation assay, two nested polymerase chain reaction (nPCR) assays and a duplex real-time quantitative polymerase chain reaction (qPCR) assay to detect A. marginale and A. centrale infections in cattle (n = 66) in South Africa. The lowest detection limits for A. marginale plasmid DNA were 2500 copies by the RLB assay, 250 copies by the nPCR and qPCR assays and 2500, 250 and 25 copies of A. centrale plasmid DNA by the RLB, nPCR and qPCR assays respectively. The qPCR assay detected more A. marginale- and A. centrale-positive samples than the other assays, either as single or mixed infections. Although the results of the qPCR and nPCR tests were in agreement for the majority (38) of A. marginale-positive samples, 13 samples tested negative for A. marginale using nPCR but positive using qPCR. To explain this discrepancy, the target sequence region of the nPCR assay was evaluated by cloning and sequencing the msp1β gene from selected field samples. The results indicated sequence variation in the internal forward primer (AM100) area amongst the South African A. marginale msp1β sequences, resulting in false negatives. We propose the use of the duplex qPCR assay in future studies as it is more sensitive and offers the benefits of quantification and multiplex detection of both Anaplasma spp. AOSIS 2017-01-23 /pmc/articles/PMC6238773/ /pubmed/28155283 http://dx.doi.org/10.4102/ojvr.v84i1.1262 Text en © 2017. The Authors http://creativecommons.org/licenses/by/2.0/ Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License. |
spellingShingle | Original Research Chaisi, Mamohale E. Baxter, Janine R. Hove, Paidashe Choopa, Chimvwele N. Oosthuizen, Marinda C. Brayton, Kelly A. Khumalo, Zamantungwa T.H. Mutshembele, Awelani M. Mtshali, Moses S. Collins, Nicola E. Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle |
title | Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle |
title_full | Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle |
title_fullStr | Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle |
title_full_unstemmed | Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle |
title_short | Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle |
title_sort | comparison of three nucleic acid-based tests for detecting anaplasma marginale and anaplasma centrale in cattle |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238773/ https://www.ncbi.nlm.nih.gov/pubmed/28155283 http://dx.doi.org/10.4102/ojvr.v84i1.1262 |
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