Microscope calibration using laser written fluorescence

There is currently no widely adopted standard for the optical characterization of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written...

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Detalles Bibliográficos
Autores principales: Corbett, Alexander D., Shaw, Michael, Yacoot, Andrew, Jefferson, Andrew, Schermelleh, Lothar, Wilson, Tony, Booth, Martin, Salter, Patrick S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238825/
https://www.ncbi.nlm.nih.gov/pubmed/30130891
http://dx.doi.org/10.1364/OE.26.021887
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author Corbett, Alexander D.
Shaw, Michael
Yacoot, Andrew
Jefferson, Andrew
Schermelleh, Lothar
Wilson, Tony
Booth, Martin
Salter, Patrick S.
author_facet Corbett, Alexander D.
Shaw, Michael
Yacoot, Andrew
Jefferson, Andrew
Schermelleh, Lothar
Wilson, Tony
Booth, Martin
Salter, Patrick S.
author_sort Corbett, Alexander D.
collection PubMed
description There is currently no widely adopted standard for the optical characterization of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and color plane alignment using confocal and structured illumination fluorescence microscopes.
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spelling pubmed-62388252019-08-08 Microscope calibration using laser written fluorescence Corbett, Alexander D. Shaw, Michael Yacoot, Andrew Jefferson, Andrew Schermelleh, Lothar Wilson, Tony Booth, Martin Salter, Patrick S. Opt Express Article There is currently no widely adopted standard for the optical characterization of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and color plane alignment using confocal and structured illumination fluorescence microscopes. Optical Society of America 2018-08-08 /pmc/articles/PMC6238825/ /pubmed/30130891 http://dx.doi.org/10.1364/OE.26.021887 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) . Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.
spellingShingle Article
Corbett, Alexander D.
Shaw, Michael
Yacoot, Andrew
Jefferson, Andrew
Schermelleh, Lothar
Wilson, Tony
Booth, Martin
Salter, Patrick S.
Microscope calibration using laser written fluorescence
title Microscope calibration using laser written fluorescence
title_full Microscope calibration using laser written fluorescence
title_fullStr Microscope calibration using laser written fluorescence
title_full_unstemmed Microscope calibration using laser written fluorescence
title_short Microscope calibration using laser written fluorescence
title_sort microscope calibration using laser written fluorescence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238825/
https://www.ncbi.nlm.nih.gov/pubmed/30130891
http://dx.doi.org/10.1364/OE.26.021887
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