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Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy
Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence str...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240104/ https://www.ncbi.nlm.nih.gov/pubmed/30446673 http://dx.doi.org/10.1038/s41467-018-07244-4 |
| _version_ | 1783371573921579008 |
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| author | Chen, Youhua Liu, Wenjie Zhang, Zhimin Zheng, Cheng Huang, Yujia Cao, Ruizhi Zhu, Dazhao Xu, Liang Zhang, Meng Zhang, Yu-Hui Fan, Jiannan Jin, Luhong Xu, Yingke Kuang, Cuifang Liu, Xu |
| author_facet | Chen, Youhua Liu, Wenjie Zhang, Zhimin Zheng, Cheng Huang, Yujia Cao, Ruizhi Zhu, Dazhao Xu, Liang Zhang, Meng Zhang, Yu-Hui Fan, Jiannan Jin, Luhong Xu, Yingke Kuang, Cuifang Liu, Xu |
| author_sort | Chen, Youhua |
| collection | PubMed |
| description | Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dynamics of mitochondria and microtubule architectures during cell migration. |
| format | Online Article Text |
| id | pubmed-6240104 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-62401042018-11-19 Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy Chen, Youhua Liu, Wenjie Zhang, Zhimin Zheng, Cheng Huang, Yujia Cao, Ruizhi Zhu, Dazhao Xu, Liang Zhang, Meng Zhang, Yu-Hui Fan, Jiannan Jin, Luhong Xu, Yingke Kuang, Cuifang Liu, Xu Nat Commun Article Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dynamics of mitochondria and microtubule architectures during cell migration. Nature Publishing Group UK 2018-11-16 /pmc/articles/PMC6240104/ /pubmed/30446673 http://dx.doi.org/10.1038/s41467-018-07244-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Article Chen, Youhua Liu, Wenjie Zhang, Zhimin Zheng, Cheng Huang, Yujia Cao, Ruizhi Zhu, Dazhao Xu, Liang Zhang, Meng Zhang, Yu-Hui Fan, Jiannan Jin, Luhong Xu, Yingke Kuang, Cuifang Liu, Xu Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy |
| title | Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy |
| title_full | Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy |
| title_fullStr | Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy |
| title_full_unstemmed | Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy |
| title_short | Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy |
| title_sort | multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240104/ https://www.ncbi.nlm.nih.gov/pubmed/30446673 http://dx.doi.org/10.1038/s41467-018-07244-4 |
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