Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon

OBJECTIVE: Dried blood spots (DBS) offer multiple benefits for collecting, storing and shipping whole blood samples. Our objective was to compare, for the first time in Africa, the performance of DBS with respect to plasma in the detection of Hepatitis B surface antigen (HBsAg) and antibodies to Hep...

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Autores principales: Kenmoe, Sebastien, Tagnouokam, Paul Alain Ngoupo, Nde, Cyprien Kengne, Mella-Tamko, Ghislaine Flore, Njouom, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240176/
https://www.ncbi.nlm.nih.gov/pubmed/30446000
http://dx.doi.org/10.1186/s13104-018-3931-3
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author Kenmoe, Sebastien
Tagnouokam, Paul Alain Ngoupo
Nde, Cyprien Kengne
Mella-Tamko, Ghislaine Flore
Njouom, Richard
author_facet Kenmoe, Sebastien
Tagnouokam, Paul Alain Ngoupo
Nde, Cyprien Kengne
Mella-Tamko, Ghislaine Flore
Njouom, Richard
author_sort Kenmoe, Sebastien
collection PubMed
description OBJECTIVE: Dried blood spots (DBS) offer multiple benefits for collecting, storing and shipping whole blood samples. Our objective was to compare, for the first time in Africa, the performance of DBS with respect to plasma in the detection of Hepatitis B surface antigen (HBsAg) and antibodies to Hepatitis C Virus (anti-HCV) using Architect, Abbott Diagnostics. RESULTS: DBS had a sensitivity of 99%, a specificity of 100%, a positive predictive value of 99%, a negative predictive value of 100% and a kappa index of 0.99 for the detection of HBsAg. For anti-HCV detection, the sensitivity, specificity, positive predictive value, negative predictive value and kappa index were 99%, 98%, 98%, 99%, and 0.97, respectively. This study confirms that DBS may be a reliable alternative specimen type for HBV and HCV diagnosis.
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spelling pubmed-62401762018-11-26 Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon Kenmoe, Sebastien Tagnouokam, Paul Alain Ngoupo Nde, Cyprien Kengne Mella-Tamko, Ghislaine Flore Njouom, Richard BMC Res Notes Research Note OBJECTIVE: Dried blood spots (DBS) offer multiple benefits for collecting, storing and shipping whole blood samples. Our objective was to compare, for the first time in Africa, the performance of DBS with respect to plasma in the detection of Hepatitis B surface antigen (HBsAg) and antibodies to Hepatitis C Virus (anti-HCV) using Architect, Abbott Diagnostics. RESULTS: DBS had a sensitivity of 99%, a specificity of 100%, a positive predictive value of 99%, a negative predictive value of 100% and a kappa index of 0.99 for the detection of HBsAg. For anti-HCV detection, the sensitivity, specificity, positive predictive value, negative predictive value and kappa index were 99%, 98%, 98%, 99%, and 0.97, respectively. This study confirms that DBS may be a reliable alternative specimen type for HBV and HCV diagnosis. BioMed Central 2018-11-16 /pmc/articles/PMC6240176/ /pubmed/30446000 http://dx.doi.org/10.1186/s13104-018-3931-3 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Kenmoe, Sebastien
Tagnouokam, Paul Alain Ngoupo
Nde, Cyprien Kengne
Mella-Tamko, Ghislaine Flore
Njouom, Richard
Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon
title Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon
title_full Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon
title_fullStr Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon
title_full_unstemmed Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon
title_short Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon
title_sort using dried blood spot for the detection of hbsag and anti-hcv antibodies in cameroon
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240176/
https://www.ncbi.nlm.nih.gov/pubmed/30446000
http://dx.doi.org/10.1186/s13104-018-3931-3
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