Cargando…

Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility

BACKGROUND: To investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete sterility owing to a dozen of hybrid sterility loci, termed HS loci, but this...

Descripción completa

Detalles Bibliográficos
Autores principales: Kanaoka, Yoshitaka, Kuniyoshi, Daichi, Inada, Eri, Koide, Yohei, Okamoto, Yoshihiro, Yasui, Hideshi, Kishima, Yuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240274/
https://www.ncbi.nlm.nih.gov/pubmed/30473723
http://dx.doi.org/10.1186/s13007-018-0370-z
_version_ 1783371611543437312
author Kanaoka, Yoshitaka
Kuniyoshi, Daichi
Inada, Eri
Koide, Yohei
Okamoto, Yoshihiro
Yasui, Hideshi
Kishima, Yuji
author_facet Kanaoka, Yoshitaka
Kuniyoshi, Daichi
Inada, Eri
Koide, Yohei
Okamoto, Yoshihiro
Yasui, Hideshi
Kishima, Yuji
author_sort Kanaoka, Yoshitaka
collection PubMed
description BACKGROUND: To investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete sterility owing to a dozen of hybrid sterility loci, termed HS loci, but this complicated genetic system remains poorly understood. RESULTS: Microspores from these interspecific hybrids form sterile pollen but are viable at the immature stage. Application of the anther culture (AC) method caused these immature microspores to induce callus. The segregation distortion of 11 among 13 known HS loci was assessed in the callus population. Using many individual calli, fine mapping of the HS loci was attempted based on heterozygotes produced from chromosome segment substitution lines (CSSLs). Transmission ratio distortion (TRD) from microspores was detected at 6 of 11 HS loci in the callus population. The fine mapping of S(1) and S(19) loci using CSSLs revealed precise distances of markers from the positions of HS loci exhibiting excessive TRD. CONCLUSIONS: We demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple HS loci and dramatically shortened the process for mapping hybrid sterility genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13007-018-0370-z) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-6240274
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-62402742018-11-23 Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility Kanaoka, Yoshitaka Kuniyoshi, Daichi Inada, Eri Koide, Yohei Okamoto, Yoshihiro Yasui, Hideshi Kishima, Yuji Plant Methods Research BACKGROUND: To investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete sterility owing to a dozen of hybrid sterility loci, termed HS loci, but this complicated genetic system remains poorly understood. RESULTS: Microspores from these interspecific hybrids form sterile pollen but are viable at the immature stage. Application of the anther culture (AC) method caused these immature microspores to induce callus. The segregation distortion of 11 among 13 known HS loci was assessed in the callus population. Using many individual calli, fine mapping of the HS loci was attempted based on heterozygotes produced from chromosome segment substitution lines (CSSLs). Transmission ratio distortion (TRD) from microspores was detected at 6 of 11 HS loci in the callus population. The fine mapping of S(1) and S(19) loci using CSSLs revealed precise distances of markers from the positions of HS loci exhibiting excessive TRD. CONCLUSIONS: We demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple HS loci and dramatically shortened the process for mapping hybrid sterility genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13007-018-0370-z) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-17 /pmc/articles/PMC6240274/ /pubmed/30473723 http://dx.doi.org/10.1186/s13007-018-0370-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Kanaoka, Yoshitaka
Kuniyoshi, Daichi
Inada, Eri
Koide, Yohei
Okamoto, Yoshihiro
Yasui, Hideshi
Kishima, Yuji
Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
title Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
title_full Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
title_fullStr Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
title_full_unstemmed Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
title_short Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
title_sort anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240274/
https://www.ncbi.nlm.nih.gov/pubmed/30473723
http://dx.doi.org/10.1186/s13007-018-0370-z
work_keys_str_mv AT kanaokayoshitaka anthercultureinriceproportionallyrescuesmicrosporesaccordingtogametophyticgeneeffectandenhancesgeneticstudyofhybridsterility
AT kuniyoshidaichi anthercultureinriceproportionallyrescuesmicrosporesaccordingtogametophyticgeneeffectandenhancesgeneticstudyofhybridsterility
AT inadaeri anthercultureinriceproportionallyrescuesmicrosporesaccordingtogametophyticgeneeffectandenhancesgeneticstudyofhybridsterility
AT koideyohei anthercultureinriceproportionallyrescuesmicrosporesaccordingtogametophyticgeneeffectandenhancesgeneticstudyofhybridsterility
AT okamotoyoshihiro anthercultureinriceproportionallyrescuesmicrosporesaccordingtogametophyticgeneeffectandenhancesgeneticstudyofhybridsterility
AT yasuihideshi anthercultureinriceproportionallyrescuesmicrosporesaccordingtogametophyticgeneeffectandenhancesgeneticstudyofhybridsterility
AT kishimayuji anthercultureinriceproportionallyrescuesmicrosporesaccordingtogametophyticgeneeffectandenhancesgeneticstudyofhybridsterility