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Fifteen microsatellite markers for the Appalachian rockcap fern, Polypodium appalachianum (Polypodiaceae), and its relatives
PREMISE OF THE STUDY: Microsatellite markers were developed for Polypodium appalachianum (Polypodiaceae) to facilitate investigation of species boundaries between P. appalachianum and its putative hybrid, P. virginianum, and potentially among other members of the Miocene‐age P. vulgare species compl...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240454/ https://www.ncbi.nlm.nih.gov/pubmed/30473941 http://dx.doi.org/10.1002/aps3.1195 |
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author | Kasireddy, Vishnupriya Mitchell, Erika Murrell, Zack E. Gillespie, Emily L. |
author_facet | Kasireddy, Vishnupriya Mitchell, Erika Murrell, Zack E. Gillespie, Emily L. |
author_sort | Kasireddy, Vishnupriya |
collection | PubMed |
description | PREMISE OF THE STUDY: Microsatellite markers were developed for Polypodium appalachianum (Polypodiaceae) to facilitate investigation of species boundaries between P. appalachianum and its putative hybrid, P. virginianum, and potentially among other members of the Miocene‐age P. vulgare species complex. METHODS AND RESULTS: Forty‐eight primer pairs were designed from Illumina data and screened for successful amplification. Sixteen pairs were genotyped and evaluated for variability within and among three populations in North Carolina, Vermont, and New Hampshire. Twelve of these primer pairs were reliable and polymorphic, exhibiting one to 10 alleles per locus. Cross‐species amplification experiments were conducted for P. virginianum and four additional close relatives from the P. vulgare complex in order to maximize information about likely utility within a phylogenetic context. CONCLUSIONS: These microsatellite markers will be useful in population genetics and species boundaries studies of P. appalachianum and P. virginianum, and likely in other species within the P. vulgare complex. |
format | Online Article Text |
id | pubmed-6240454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62404542018-11-23 Fifteen microsatellite markers for the Appalachian rockcap fern, Polypodium appalachianum (Polypodiaceae), and its relatives Kasireddy, Vishnupriya Mitchell, Erika Murrell, Zack E. Gillespie, Emily L. Appl Plant Sci Primer Notes PREMISE OF THE STUDY: Microsatellite markers were developed for Polypodium appalachianum (Polypodiaceae) to facilitate investigation of species boundaries between P. appalachianum and its putative hybrid, P. virginianum, and potentially among other members of the Miocene‐age P. vulgare species complex. METHODS AND RESULTS: Forty‐eight primer pairs were designed from Illumina data and screened for successful amplification. Sixteen pairs were genotyped and evaluated for variability within and among three populations in North Carolina, Vermont, and New Hampshire. Twelve of these primer pairs were reliable and polymorphic, exhibiting one to 10 alleles per locus. Cross‐species amplification experiments were conducted for P. virginianum and four additional close relatives from the P. vulgare complex in order to maximize information about likely utility within a phylogenetic context. CONCLUSIONS: These microsatellite markers will be useful in population genetics and species boundaries studies of P. appalachianum and P. virginianum, and likely in other species within the P. vulgare complex. John Wiley and Sons Inc. 2018-11-08 /pmc/articles/PMC6240454/ /pubmed/30473941 http://dx.doi.org/10.1002/aps3.1195 Text en © 2018 Kasireddy et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Primer Notes Kasireddy, Vishnupriya Mitchell, Erika Murrell, Zack E. Gillespie, Emily L. Fifteen microsatellite markers for the Appalachian rockcap fern, Polypodium appalachianum (Polypodiaceae), and its relatives |
title | Fifteen microsatellite markers for the Appalachian rockcap fern, Polypodium appalachianum (Polypodiaceae), and its relatives |
title_full | Fifteen microsatellite markers for the Appalachian rockcap fern, Polypodium appalachianum (Polypodiaceae), and its relatives |
title_fullStr | Fifteen microsatellite markers for the Appalachian rockcap fern, Polypodium appalachianum (Polypodiaceae), and its relatives |
title_full_unstemmed | Fifteen microsatellite markers for the Appalachian rockcap fern, Polypodium appalachianum (Polypodiaceae), and its relatives |
title_short | Fifteen microsatellite markers for the Appalachian rockcap fern, Polypodium appalachianum (Polypodiaceae), and its relatives |
title_sort | fifteen microsatellite markers for the appalachian rockcap fern, polypodium appalachianum (polypodiaceae), and its relatives |
topic | Primer Notes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240454/ https://www.ncbi.nlm.nih.gov/pubmed/30473941 http://dx.doi.org/10.1002/aps3.1195 |
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