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RPS15a Silencing Suppresses Cell Proliferation and Migration of Gastric Cancer
PURPOSE: Information on the possible role of the ribosomal protein S15a (RPS15a) in gastric cancer is scarce. The aim of this study was to evaluate the impact of RPS15a gene expression on the growth and cell cycle of gastric cancer cells in vitro and in vivo. MATERIALS AND METHODS: RPS15a mRNA expre...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Yonsei University College of Medicine
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240561/ https://www.ncbi.nlm.nih.gov/pubmed/30450850 http://dx.doi.org/10.3349/ymj.2018.59.10.1166 |
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author | Shi, Ding Liu, Jinjin |
author_facet | Shi, Ding Liu, Jinjin |
author_sort | Shi, Ding |
collection | PubMed |
description | PURPOSE: Information on the possible role of the ribosomal protein S15a (RPS15a) in gastric cancer is scarce. The aim of this study was to evaluate the impact of RPS15a gene expression on the growth and cell cycle of gastric cancer cells in vitro and in vivo. MATERIALS AND METHODS: RPS15a mRNA expression was examined in cancer tissues and their corresponding adjacent normal tissues of 40 gastric adenocarcinoma patients. Next, RPS15a was knocked down using a lentivirus-mediated RNA interference (short hairpin RNA) system in the gastric cancer cell line BGC823. The effect of RPS15a knockdown was examined using CCK-8 assay, cell scratch test, colony formation assay, and flow cytometry. Finally, in nude mice, a tumorigenicity test was performed, and the tumor volume and weight were measured. RESULTS: RPS15a expression in tumor tissue was significantly greater than that in the adjacent normal tissue of gastric cancer patients. After RPS15a silencing, the BGC823 cell proliferation rate decreased significantly; most cells were arrested in the G0/G1 phase, cell growth was inhibited, and the migration rate was decreased. Colony formation assay showed that the number and size of clones in the RPS15a-silenced cells were fewer and smaller, compared to control cells. The nude mouse tumorigenicity test showed that RPS15a silencing had an inhibitory effect on tumor volume and mice weight. CONCLUSION: The present study found RPS15a expression to be higher in gastric tumors and its silencing in gastric cancer cells to inhibit the proliferation, growth, and migration thereof. Accordingly, RPS15a may be considered as a potential therapeutic target in gastric cancer. |
format | Online Article Text |
id | pubmed-6240561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Yonsei University College of Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-62405612018-12-01 RPS15a Silencing Suppresses Cell Proliferation and Migration of Gastric Cancer Shi, Ding Liu, Jinjin Yonsei Med J Original Article PURPOSE: Information on the possible role of the ribosomal protein S15a (RPS15a) in gastric cancer is scarce. The aim of this study was to evaluate the impact of RPS15a gene expression on the growth and cell cycle of gastric cancer cells in vitro and in vivo. MATERIALS AND METHODS: RPS15a mRNA expression was examined in cancer tissues and their corresponding adjacent normal tissues of 40 gastric adenocarcinoma patients. Next, RPS15a was knocked down using a lentivirus-mediated RNA interference (short hairpin RNA) system in the gastric cancer cell line BGC823. The effect of RPS15a knockdown was examined using CCK-8 assay, cell scratch test, colony formation assay, and flow cytometry. Finally, in nude mice, a tumorigenicity test was performed, and the tumor volume and weight were measured. RESULTS: RPS15a expression in tumor tissue was significantly greater than that in the adjacent normal tissue of gastric cancer patients. After RPS15a silencing, the BGC823 cell proliferation rate decreased significantly; most cells were arrested in the G0/G1 phase, cell growth was inhibited, and the migration rate was decreased. Colony formation assay showed that the number and size of clones in the RPS15a-silenced cells were fewer and smaller, compared to control cells. The nude mouse tumorigenicity test showed that RPS15a silencing had an inhibitory effect on tumor volume and mice weight. CONCLUSION: The present study found RPS15a expression to be higher in gastric tumors and its silencing in gastric cancer cells to inhibit the proliferation, growth, and migration thereof. Accordingly, RPS15a may be considered as a potential therapeutic target in gastric cancer. Yonsei University College of Medicine 2018-12-01 2018-11-15 /pmc/articles/PMC6240561/ /pubmed/30450850 http://dx.doi.org/10.3349/ymj.2018.59.10.1166 Text en © Copyright: Yonsei University College of Medicine 2018 https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Shi, Ding Liu, Jinjin RPS15a Silencing Suppresses Cell Proliferation and Migration of Gastric Cancer |
title | RPS15a Silencing Suppresses Cell Proliferation and Migration of Gastric Cancer |
title_full | RPS15a Silencing Suppresses Cell Proliferation and Migration of Gastric Cancer |
title_fullStr | RPS15a Silencing Suppresses Cell Proliferation and Migration of Gastric Cancer |
title_full_unstemmed | RPS15a Silencing Suppresses Cell Proliferation and Migration of Gastric Cancer |
title_short | RPS15a Silencing Suppresses Cell Proliferation and Migration of Gastric Cancer |
title_sort | rps15a silencing suppresses cell proliferation and migration of gastric cancer |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240561/ https://www.ncbi.nlm.nih.gov/pubmed/30450850 http://dx.doi.org/10.3349/ymj.2018.59.10.1166 |
work_keys_str_mv | AT shiding rps15asilencingsuppressescellproliferationandmigrationofgastriccancer AT liujinjin rps15asilencingsuppressescellproliferationandmigrationofgastriccancer |