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Spectroscopic Characterization of an Extensive Set of c-Type Peptide Fragment Ions Formed by Electron Transfer Dissociation Suggests Exclusive Formation of Amide Isomers
[Image: see text] Electron attachment dissociation (electron capture dissociation (ECD) and electron transfer dissociation (ETD)) applied to gaseous multiply protonated peptides leads predominantly to backbone N–C(α) bond cleavages and the formation of c- and z-type fragment ions. The mechanisms inv...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240889/ https://www.ncbi.nlm.nih.gov/pubmed/30343579 http://dx.doi.org/10.1021/acs.jpclett.8b02850 |
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author | Kempkes, Lisanne J. M. Martens, Jonathan Berden, Giel Oomens, Jos |
author_facet | Kempkes, Lisanne J. M. Martens, Jonathan Berden, Giel Oomens, Jos |
author_sort | Kempkes, Lisanne J. M. |
collection | PubMed |
description | [Image: see text] Electron attachment dissociation (electron capture dissociation (ECD) and electron transfer dissociation (ETD)) applied to gaseous multiply protonated peptides leads predominantly to backbone N–C(α) bond cleavages and the formation of c- and z-type fragment ions. The mechanisms involved in the formation of these ions have been the subject of much discussion. Here, we determine the molecular structures of an extensive set of c-type ions produced by ETD using infrared ion spectroscopy. Nine c(3)- and c(4)-ions are investigated to establish their C-terminal structure as either enol-imine or amide isomers by comparison of the experimental infrared spectra with quantum-chemically predicted spectra for both structural variants. The spectra suggest that all c-ions investigated possess an amide structure; the absence of the NH bending mode at approximately 1000–1200 cm(–1) serves as an important diagnostic feature. |
format | Online Article Text |
id | pubmed-6240889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-62408892018-11-20 Spectroscopic Characterization of an Extensive Set of c-Type Peptide Fragment Ions Formed by Electron Transfer Dissociation Suggests Exclusive Formation of Amide Isomers Kempkes, Lisanne J. M. Martens, Jonathan Berden, Giel Oomens, Jos J Phys Chem Lett [Image: see text] Electron attachment dissociation (electron capture dissociation (ECD) and electron transfer dissociation (ETD)) applied to gaseous multiply protonated peptides leads predominantly to backbone N–C(α) bond cleavages and the formation of c- and z-type fragment ions. The mechanisms involved in the formation of these ions have been the subject of much discussion. Here, we determine the molecular structures of an extensive set of c-type ions produced by ETD using infrared ion spectroscopy. Nine c(3)- and c(4)-ions are investigated to establish their C-terminal structure as either enol-imine or amide isomers by comparison of the experimental infrared spectra with quantum-chemically predicted spectra for both structural variants. The spectra suggest that all c-ions investigated possess an amide structure; the absence of the NH bending mode at approximately 1000–1200 cm(–1) serves as an important diagnostic feature. American Chemical Society 2018-10-22 2018-11-15 /pmc/articles/PMC6240889/ /pubmed/30343579 http://dx.doi.org/10.1021/acs.jpclett.8b02850 Text en Copyright © 2018 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Kempkes, Lisanne J. M. Martens, Jonathan Berden, Giel Oomens, Jos Spectroscopic Characterization of an Extensive Set of c-Type Peptide Fragment Ions Formed by Electron Transfer Dissociation Suggests Exclusive Formation of Amide Isomers |
title | Spectroscopic Characterization of an Extensive Set
of c-Type Peptide Fragment Ions Formed by
Electron Transfer Dissociation Suggests Exclusive Formation of Amide
Isomers |
title_full | Spectroscopic Characterization of an Extensive Set
of c-Type Peptide Fragment Ions Formed by
Electron Transfer Dissociation Suggests Exclusive Formation of Amide
Isomers |
title_fullStr | Spectroscopic Characterization of an Extensive Set
of c-Type Peptide Fragment Ions Formed by
Electron Transfer Dissociation Suggests Exclusive Formation of Amide
Isomers |
title_full_unstemmed | Spectroscopic Characterization of an Extensive Set
of c-Type Peptide Fragment Ions Formed by
Electron Transfer Dissociation Suggests Exclusive Formation of Amide
Isomers |
title_short | Spectroscopic Characterization of an Extensive Set
of c-Type Peptide Fragment Ions Formed by
Electron Transfer Dissociation Suggests Exclusive Formation of Amide
Isomers |
title_sort | spectroscopic characterization of an extensive set
of c-type peptide fragment ions formed by
electron transfer dissociation suggests exclusive formation of amide
isomers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240889/ https://www.ncbi.nlm.nih.gov/pubmed/30343579 http://dx.doi.org/10.1021/acs.jpclett.8b02850 |
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